The key role of sulfation and branching on fucoidan antitumor activity
Autor(a) principal: | |
---|---|
Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/44382 |
Resumo: | There is an urgent need for antitumor bioactive agents with minimal or no side effects over normal adjacent cells. Fucoidan is a marine-origin polymer with known antitumor activity. However, there are still some concerns about its application due to the inconsistent experimental results, specifically its toxicity over normal cells and the mechanism behind its action. Herein, three fucoidan extracts (FEs) have been tested over normal and breast cancer cell lines. From cytotoxicity results, only one of the extracts shows selective antitumor behavior (at 0.2 mg mLâ 1), despite similarities in sulfation degree and carbohydrates composition. Although the three FEs present different molecular weights, depolymerization of selected samples discarded Mw as the key factor in the antitumor activity. Significant differences in sulfates position and branching are observed, presenting FE 2 the higher branching degree. Based on all these experimental data, it is believed that these last two properties are the ones that influence the cytotoxic effects of fucoidan extracts. |
id |
RCAP_37ddd96311f3cf9eba675a20ef3ee85f |
---|---|
oai_identifier_str |
oai:repositorium.sdum.uminho.pt:1822/44382 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
The key role of sulfation and branching on fucoidan antitumor activityAntitumor activityFucoidanPolymer branchingStructure–activity relationshipSulfationScience & TechnologyThere is an urgent need for antitumor bioactive agents with minimal or no side effects over normal adjacent cells. Fucoidan is a marine-origin polymer with known antitumor activity. However, there are still some concerns about its application due to the inconsistent experimental results, specifically its toxicity over normal cells and the mechanism behind its action. Herein, three fucoidan extracts (FEs) have been tested over normal and breast cancer cell lines. From cytotoxicity results, only one of the extracts shows selective antitumor behavior (at 0.2 mg mLâ 1), despite similarities in sulfation degree and carbohydrates composition. Although the three FEs present different molecular weights, depolymerization of selected samples discarded Mw as the key factor in the antitumor activity. Significant differences in sulfates position and branching are observed, presenting FE 2 the higher branching degree. Based on all these experimental data, it is believed that these last two properties are the ones that influence the cytotoxic effects of fucoidan extracts.The authors would like to thank the funding from projects 0687_NOVOMAR_1_P, cofunded by INTERREG 2007-2013/POCTEP, CarbPol_u_Algae (EXPL/MAR-BIO/0165/2013), and IF/00376/2014/CP1212/CT0015, funded by the Portuguese Foundation for Science and Technology, FCT, and ComplexiTE (ERC-2012-ADG 20120216-321266), funded by the European Research Council under the European Union's Seventh Framework Programme for Research and Development. The authors would also like to thank FCT, Portugal, for the scholarship of A.S.F. (SFRH/BD/102471/2014), fellowship of C.N. (SFRH/BPD/100627/2014), Investigator grants of A.M. (IF/00376/2014), R.N.-C. (IF/00373/2014), and I.P. (IF/00032/2013) and the financial support to CICECO-Aveiro Institute of Materials (POCI-01-0145-FEDER-007679, FCT UID/CTM/50011/2013) and OOPNA (UID/OUI/00062/2013), through national founds and cofinanced by the FEDER, within the PT2020 Partnership Agreement.John Wiley and SonsUniversidade do MinhoOliveira, CatarinaFerreira, Andreia S.Novoa-Carballal, R.Nunes, CláudiaPashkuleva, I.Neves, N. M.Coimbra, Manuel A.Reis, R. L.Martins, AlbinoSilva, Tiago José Quinteiros Lopes Henriques20172017-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/44382engOliveira C., Ferreira A. S., Novoa-Carballal R., Nunes C., Pashkuleva I., Neves N. M., Coimbra M. A., Reis R. L., Martins A., Silva T. H. The Key Role of Sulfation and Branching on Fucoidan Antitumor Activity, Macromolecular Bioscience, doi:10.1002/mabi.201600340, 20161616-519510.1002/mabi.20160034027996198http://dx.doi.org/10.1002/mabi.201600340info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:17:44Zoai:repositorium.sdum.uminho.pt:1822/44382Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:10:24.704752Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
The key role of sulfation and branching on fucoidan antitumor activity |
title |
The key role of sulfation and branching on fucoidan antitumor activity |
spellingShingle |
The key role of sulfation and branching on fucoidan antitumor activity Oliveira, Catarina Antitumor activity Fucoidan Polymer branching Structure–activity relationship Sulfation Science & Technology |
title_short |
The key role of sulfation and branching on fucoidan antitumor activity |
title_full |
The key role of sulfation and branching on fucoidan antitumor activity |
title_fullStr |
The key role of sulfation and branching on fucoidan antitumor activity |
title_full_unstemmed |
The key role of sulfation and branching on fucoidan antitumor activity |
title_sort |
The key role of sulfation and branching on fucoidan antitumor activity |
author |
Oliveira, Catarina |
author_facet |
Oliveira, Catarina Ferreira, Andreia S. Novoa-Carballal, R. Nunes, Cláudia Pashkuleva, I. Neves, N. M. Coimbra, Manuel A. Reis, R. L. Martins, Albino Silva, Tiago José Quinteiros Lopes Henriques |
author_role |
author |
author2 |
Ferreira, Andreia S. Novoa-Carballal, R. Nunes, Cláudia Pashkuleva, I. Neves, N. M. Coimbra, Manuel A. Reis, R. L. Martins, Albino Silva, Tiago José Quinteiros Lopes Henriques |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Oliveira, Catarina Ferreira, Andreia S. Novoa-Carballal, R. Nunes, Cláudia Pashkuleva, I. Neves, N. M. Coimbra, Manuel A. Reis, R. L. Martins, Albino Silva, Tiago José Quinteiros Lopes Henriques |
dc.subject.por.fl_str_mv |
Antitumor activity Fucoidan Polymer branching Structure–activity relationship Sulfation Science & Technology |
topic |
Antitumor activity Fucoidan Polymer branching Structure–activity relationship Sulfation Science & Technology |
description |
There is an urgent need for antitumor bioactive agents with minimal or no side effects over normal adjacent cells. Fucoidan is a marine-origin polymer with known antitumor activity. However, there are still some concerns about its application due to the inconsistent experimental results, specifically its toxicity over normal cells and the mechanism behind its action. Herein, three fucoidan extracts (FEs) have been tested over normal and breast cancer cell lines. From cytotoxicity results, only one of the extracts shows selective antitumor behavior (at 0.2 mg mLâ 1), despite similarities in sulfation degree and carbohydrates composition. Although the three FEs present different molecular weights, depolymerization of selected samples discarded Mw as the key factor in the antitumor activity. Significant differences in sulfates position and branching are observed, presenting FE 2 the higher branching degree. Based on all these experimental data, it is believed that these last two properties are the ones that influence the cytotoxic effects of fucoidan extracts. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017 2017-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/44382 |
url |
http://hdl.handle.net/1822/44382 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Oliveira C., Ferreira A. S., Novoa-Carballal R., Nunes C., Pashkuleva I., Neves N. M., Coimbra M. A., Reis R. L., Martins A., Silva T. H. The Key Role of Sulfation and Branching on Fucoidan Antitumor Activity, Macromolecular Bioscience, doi:10.1002/mabi.201600340, 2016 1616-5195 10.1002/mabi.201600340 27996198 http://dx.doi.org/10.1002/mabi.201600340 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
John Wiley and Sons |
publisher.none.fl_str_mv |
John Wiley and Sons |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799132533402632192 |