HER2 expression in circulating tumour cells isolated from metastatic breast cancer patients using a size-based microfluidic device

Detalhes bibliográficos
Autor(a) principal: Lopes, Cláudia
Data de Publicação: 2021
Outros Autores: Piairo, Paulina, Chícharo, Alexandre, Abalde-Cela, Sara, Pires, Liliana R., Corredeira, Patrícia, Alves, Patrícia, Muinelo-Romay, Laura, Costa, Luis, Diéguez, Lorena
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/49548
Resumo: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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spelling HER2 expression in circulating tumour cells isolated from metastatic breast cancer patients using a size-based microfluidic deviceHER2Breast cancerCirculating tumour cellsLiquid biopsyMicrofluidicsOverall survival© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).HER2 is a prognostic and predictive biomarker in breast cancer, normally assessed in tumour biopsy and used to guide treatment choices. Circulating tumour cells (CTCs) escape the primary tumour and enter the bloodstream, exhibiting great metastatic potential and representing a real-time snapshot of the tumour burden. Liquid biopsy offers the unique opportunity for low invasive sampling in cancer patients and holds the potential to provide valuable information for the clinical management of cancer patients. This study assesses the performance of the RUBYchip™, a microfluidic system for CTC capture based on cell size and deformability, and compares it with the only FDA-approved technology for CTC enumeration, CellSearch®. After optimising device performance, 30 whole blood samples from metastatic breast cancer patients were processed with both technologies. The expression of HER2 was assessed in isolated CTCs and compared to tissue biopsy. Results show that the RUBYchipTM was able to isolate CTCs with higher efficiency than CellSearch®, up to 10 times more, averaging all samples. An accurate evaluation of different CTC subpopulations, including HER2+ CTCs, was provided. Liquid biopsy through the use of the RUBYchipTM in the clinic can overcome the limitations of histological testing and evaluate HER2 status in patients in real-time, helping to tailor treatment during disease evolution.This work was supported by the CANCER project (NORTE-01-0145-FEDER-000029) co-funded through the NORTE-45-2015-02 program, the RESOLVE project (NORTE-01-0246-FEDER-000018) funded through the Norte2020, and the Safe-N-Medtech project (H2020-NMBP-02-2018) funded through Horizon 2020. The project leading to these results has also received funding and support from the “la Caixa” Foundation under CaixaImpulse Grant LCF/TR/CC20/52480003 (CTC-OD).MDPIRepositório da Universidade de LisboaLopes, CláudiaPiairo, PaulinaChícharo, AlexandreAbalde-Cela, SaraPires, Liliana R.Corredeira, PatríciaAlves, PatríciaMuinelo-Romay, LauraCosta, LuisDiéguez, Lorena2021-09-20T14:54:43Z20212021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/49548engCancers (Basel). 2021 Sep 3;13(17):444610.3390/cancers131744462072-6694info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:53:25Zoai:repositorio.ul.pt:10451/49548Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:01:10.621991Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv HER2 expression in circulating tumour cells isolated from metastatic breast cancer patients using a size-based microfluidic device
title HER2 expression in circulating tumour cells isolated from metastatic breast cancer patients using a size-based microfluidic device
spellingShingle HER2 expression in circulating tumour cells isolated from metastatic breast cancer patients using a size-based microfluidic device
Lopes, Cláudia
HER2
Breast cancer
Circulating tumour cells
Liquid biopsy
Microfluidics
Overall survival
title_short HER2 expression in circulating tumour cells isolated from metastatic breast cancer patients using a size-based microfluidic device
title_full HER2 expression in circulating tumour cells isolated from metastatic breast cancer patients using a size-based microfluidic device
title_fullStr HER2 expression in circulating tumour cells isolated from metastatic breast cancer patients using a size-based microfluidic device
title_full_unstemmed HER2 expression in circulating tumour cells isolated from metastatic breast cancer patients using a size-based microfluidic device
title_sort HER2 expression in circulating tumour cells isolated from metastatic breast cancer patients using a size-based microfluidic device
author Lopes, Cláudia
author_facet Lopes, Cláudia
Piairo, Paulina
Chícharo, Alexandre
Abalde-Cela, Sara
Pires, Liliana R.
Corredeira, Patrícia
Alves, Patrícia
Muinelo-Romay, Laura
Costa, Luis
Diéguez, Lorena
author_role author
author2 Piairo, Paulina
Chícharo, Alexandre
Abalde-Cela, Sara
Pires, Liliana R.
Corredeira, Patrícia
Alves, Patrícia
Muinelo-Romay, Laura
Costa, Luis
Diéguez, Lorena
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Lopes, Cláudia
Piairo, Paulina
Chícharo, Alexandre
Abalde-Cela, Sara
Pires, Liliana R.
Corredeira, Patrícia
Alves, Patrícia
Muinelo-Romay, Laura
Costa, Luis
Diéguez, Lorena
dc.subject.por.fl_str_mv HER2
Breast cancer
Circulating tumour cells
Liquid biopsy
Microfluidics
Overall survival
topic HER2
Breast cancer
Circulating tumour cells
Liquid biopsy
Microfluidics
Overall survival
description © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
publishDate 2021
dc.date.none.fl_str_mv 2021-09-20T14:54:43Z
2021
2021-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/49548
url http://hdl.handle.net/10451/49548
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Cancers (Basel). 2021 Sep 3;13(17):4446
10.3390/cancers13174446
2072-6694
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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