m6A RNA Methylation in marine plants: first insights and relevance for biological rhythms
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.1/14845 |
Resumo: | Circadian regulations are essential for enabling organisms to synchronize physiology with environmental light-dark cycles. Post-transcriptional RNA modifications still represent an understudied level of gene expression regulation in plants, although they could play crucial roles in environmental adaptation. N6-methyl-adenosine (m6A) is the most prevalent mRNA modification, established by "writer" and "eraser" proteins. It influences the clockwork in several taxa, but only few studies have been conducted in plants and none in marine plants. Here, we provided a first inventory of m6A-related genes in seagrasses and investigated daily changes in the global RNA methylation and transcript levels of writers and erasers in Cymodocea nodosa and Zostera marina. Both species showed methylation peaks during the dark period under the same photoperiod, despite exhibiting asynchronous changes in the m6A profile and related gene expression during a 24-h cycle. At contrasting latitudes, Z. marina populations displayed overlapping daily patterns of the m6A level and related gene expression. The observed rhythms are characteristic for each species and similar in populations of the same species with different photoperiods, suggesting the existence of an endogenous circadian control. Globally, our results indicate that m6A RNA methylation could widely contribute to circadian regulation in seagrasses, potentially affecting the photo-biological behaviour of these plants. |
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m6A RNA Methylation in marine plants: first insights and relevance for biological rhythmsMarine plantsm6A-methylationEpitranscriptomeRNA methyltransferasesCircadian clockCircadian regulations are essential for enabling organisms to synchronize physiology with environmental light-dark cycles. Post-transcriptional RNA modifications still represent an understudied level of gene expression regulation in plants, although they could play crucial roles in environmental adaptation. N6-methyl-adenosine (m6A) is the most prevalent mRNA modification, established by "writer" and "eraser" proteins. It influences the clockwork in several taxa, but only few studies have been conducted in plants and none in marine plants. Here, we provided a first inventory of m6A-related genes in seagrasses and investigated daily changes in the global RNA methylation and transcript levels of writers and erasers in Cymodocea nodosa and Zostera marina. Both species showed methylation peaks during the dark period under the same photoperiod, despite exhibiting asynchronous changes in the m6A profile and related gene expression during a 24-h cycle. At contrasting latitudes, Z. marina populations displayed overlapping daily patterns of the m6A level and related gene expression. The observed rhythms are characteristic for each species and similar in populations of the same species with different photoperiods, suggesting the existence of an endogenous circadian control. Globally, our results indicate that m6A RNA methylation could widely contribute to circadian regulation in seagrasses, potentially affecting the photo-biological behaviour of these plants.FCT: UIDB/04326/2020MDPISapientiaRuocco, MiriamAmbrosino, LucaJahnke, MarleneChiusano, MariaBarrote, IsabelProcaccini, GabrieleSilva, JoãoDattolo, Emanuela2020-11-25T13:42:25Z20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/14845eng1661-659610.3390/ijms21207508info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:27:11Zoai:sapientia.ualg.pt:10400.1/14845Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:05:48.357056Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
m6A RNA Methylation in marine plants: first insights and relevance for biological rhythms |
title |
m6A RNA Methylation in marine plants: first insights and relevance for biological rhythms |
spellingShingle |
m6A RNA Methylation in marine plants: first insights and relevance for biological rhythms Ruocco, Miriam Marine plants m6A-methylation Epitranscriptome RNA methyltransferases Circadian clock |
title_short |
m6A RNA Methylation in marine plants: first insights and relevance for biological rhythms |
title_full |
m6A RNA Methylation in marine plants: first insights and relevance for biological rhythms |
title_fullStr |
m6A RNA Methylation in marine plants: first insights and relevance for biological rhythms |
title_full_unstemmed |
m6A RNA Methylation in marine plants: first insights and relevance for biological rhythms |
title_sort |
m6A RNA Methylation in marine plants: first insights and relevance for biological rhythms |
author |
Ruocco, Miriam |
author_facet |
Ruocco, Miriam Ambrosino, Luca Jahnke, Marlene Chiusano, Maria Barrote, Isabel Procaccini, Gabriele Silva, João Dattolo, Emanuela |
author_role |
author |
author2 |
Ambrosino, Luca Jahnke, Marlene Chiusano, Maria Barrote, Isabel Procaccini, Gabriele Silva, João Dattolo, Emanuela |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Sapientia |
dc.contributor.author.fl_str_mv |
Ruocco, Miriam Ambrosino, Luca Jahnke, Marlene Chiusano, Maria Barrote, Isabel Procaccini, Gabriele Silva, João Dattolo, Emanuela |
dc.subject.por.fl_str_mv |
Marine plants m6A-methylation Epitranscriptome RNA methyltransferases Circadian clock |
topic |
Marine plants m6A-methylation Epitranscriptome RNA methyltransferases Circadian clock |
description |
Circadian regulations are essential for enabling organisms to synchronize physiology with environmental light-dark cycles. Post-transcriptional RNA modifications still represent an understudied level of gene expression regulation in plants, although they could play crucial roles in environmental adaptation. N6-methyl-adenosine (m6A) is the most prevalent mRNA modification, established by "writer" and "eraser" proteins. It influences the clockwork in several taxa, but only few studies have been conducted in plants and none in marine plants. Here, we provided a first inventory of m6A-related genes in seagrasses and investigated daily changes in the global RNA methylation and transcript levels of writers and erasers in Cymodocea nodosa and Zostera marina. Both species showed methylation peaks during the dark period under the same photoperiod, despite exhibiting asynchronous changes in the m6A profile and related gene expression during a 24-h cycle. At contrasting latitudes, Z. marina populations displayed overlapping daily patterns of the m6A level and related gene expression. The observed rhythms are characteristic for each species and similar in populations of the same species with different photoperiods, suggesting the existence of an endogenous circadian control. Globally, our results indicate that m6A RNA methylation could widely contribute to circadian regulation in seagrasses, potentially affecting the photo-biological behaviour of these plants. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-11-25T13:42:25Z 2020 2020-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.1/14845 |
url |
http://hdl.handle.net/10400.1/14845 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1661-6596 10.3390/ijms21207508 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
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application/pdf |
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MDPI |
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MDPI |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799133297991745536 |