Diabetic foot infections : application of a nisin-biogel to complement the activity of conventional antibiotics and antiseptics against Staphylococcus aureus biofilms

Detalhes bibliográficos
Autor(a) principal: Santos, Raquel
Data de Publicação: 2019
Outros Autores: Ruza, Diana, Cunha, Eva, Tavares, Luis, Oliveira, Manuela
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/22439
Resumo: Research Areas: Science & Technology - Other Topics
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spelling Diabetic foot infections : application of a nisin-biogel to complement the activity of conventional antibiotics and antiseptics against Staphylococcus aureus biofilmsHost-defense peptidesin-vitro activitiesChlorhexidineResistanteInterococcusVancomycinMechanismsDiagnosisResearch Areas: Science & Technology - Other TopicsABSTRACT - Background Diabetic foot infections (DFIs) are a frequent complication of Diabetes mellitus and a major cause of nontraumatic limb amputations. The Gram-positive bacterium Staphylococcus aureus, known for its resilient biofilms and antibiotic resistant profile, is the most frequent DFI pathogen. It is urgent to develop innovative treatments for these infections, being the antimicrobial peptide (AMP) nisin a potential candidate. We have previously proposed the use of a guar gum biogel as a delivery system for nisin. Here, we evaluated the potential of the nisin-biogel to enhance the efficacy of conventional antibiotics and antiseptics against DFIs S. aureus clinical isolates. Methods A collection of 23 S. aureus strains isolated from DFI patients, including multidrug- and methicillin-resistant strains, was used. The antimicrobial activity of the nisin-biogel was tested alone and in different combinations with the antiseptic chlorhexidine and the antibiotics clindamycin, gentamicin and vancomycin. Isolates' in vitro susceptibility to the different protocols was assessed using broth microdilution methods in order to determine their ability to inhibit and/or eradicate established S. aureus biofilms. Antimicrobials were added to the 96-well plates every 8 h to simulate a typical DFI treatment protocol. Statistical analysis was conducted using RCBD ANOVA in SPSS. Results The nisin-biogel showed a high antibacterial activity against biofilms formed by DFI S. aureus. The combined protocol using nisin-biogel and chlorhexidine presented the highest efficacy in biofilm formation inhibition, significantly higher (p<0.05) than the ones presented by the antibiotics-based protocols tested. Regarding biofilm eradication, there were no significant differences (p>0.05) between the activity of the combination nisin-biogel plus chlorhexidine and the conventional antibiotic-based protocols. Conclusions Results provide a valuable contribution for the development of complementary strategies to conventional antibiotics protocols. A combined protocol including chlorhexidine and nisinbiogel could be potentially applied in medical centres, contributing for the reduction of antibiotic administration, selection pressure on DFI pathogens and resistance strains dissemination.Meghan Scobee Blackledge, High Point University, UNITED STATESRepositório da Universidade de LisboaSantos, RaquelRuza, DianaCunha, EvaTavares, LuisOliveira, Manuela2021-10-28T14:56:35Z2019-07-242019-07-24T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/22439engSantos R, Ruza D, Cunha E, Tavares L, Oliveira M. 2019. Diabetic foot infections: application of a nisin-biogel to complement the activity of conventional antibiotics and antiseptics against Staphylococcus aureus biofilms. PLoS ONE 14(7):e02200001932-620310.1371/journal. pone.0220000info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:51:57Zoai:www.repository.utl.pt:10400.5/22439Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:06:51.611180Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Diabetic foot infections : application of a nisin-biogel to complement the activity of conventional antibiotics and antiseptics against Staphylococcus aureus biofilms
title Diabetic foot infections : application of a nisin-biogel to complement the activity of conventional antibiotics and antiseptics against Staphylococcus aureus biofilms
spellingShingle Diabetic foot infections : application of a nisin-biogel to complement the activity of conventional antibiotics and antiseptics against Staphylococcus aureus biofilms
Santos, Raquel
Host-defense peptides
in-vitro activities
Chlorhexidine
Resistante
Interococcus
Vancomycin
Mechanisms
Diagnosis
title_short Diabetic foot infections : application of a nisin-biogel to complement the activity of conventional antibiotics and antiseptics against Staphylococcus aureus biofilms
title_full Diabetic foot infections : application of a nisin-biogel to complement the activity of conventional antibiotics and antiseptics against Staphylococcus aureus biofilms
title_fullStr Diabetic foot infections : application of a nisin-biogel to complement the activity of conventional antibiotics and antiseptics against Staphylococcus aureus biofilms
title_full_unstemmed Diabetic foot infections : application of a nisin-biogel to complement the activity of conventional antibiotics and antiseptics against Staphylococcus aureus biofilms
title_sort Diabetic foot infections : application of a nisin-biogel to complement the activity of conventional antibiotics and antiseptics against Staphylococcus aureus biofilms
author Santos, Raquel
author_facet Santos, Raquel
Ruza, Diana
Cunha, Eva
Tavares, Luis
Oliveira, Manuela
author_role author
author2 Ruza, Diana
Cunha, Eva
Tavares, Luis
Oliveira, Manuela
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Santos, Raquel
Ruza, Diana
Cunha, Eva
Tavares, Luis
Oliveira, Manuela
dc.subject.por.fl_str_mv Host-defense peptides
in-vitro activities
Chlorhexidine
Resistante
Interococcus
Vancomycin
Mechanisms
Diagnosis
topic Host-defense peptides
in-vitro activities
Chlorhexidine
Resistante
Interococcus
Vancomycin
Mechanisms
Diagnosis
description Research Areas: Science & Technology - Other Topics
publishDate 2019
dc.date.none.fl_str_mv 2019-07-24
2019-07-24T00:00:00Z
2021-10-28T14:56:35Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/22439
url http://hdl.handle.net/10400.5/22439
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Santos R, Ruza D, Cunha E, Tavares L, Oliveira M. 2019. Diabetic foot infections: application of a nisin-biogel to complement the activity of conventional antibiotics and antiseptics against Staphylococcus aureus biofilms. PLoS ONE 14(7):e0220000
1932-6203
10.1371/journal. pone.0220000
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Meghan Scobee Blackledge, High Point University, UNITED STATES
publisher.none.fl_str_mv Meghan Scobee Blackledge, High Point University, UNITED STATES
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799131161270681600

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