The Toll/NF-kappa B signaling pathway is required for epidermal wound repair in Drosophila
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/24296 |
Resumo: | The Toll/NF-kappa B pathway, first identified in studies of dorsal-ventral polarity in the early Drosophila embryo, is well known for its role in the innate immune response. Here, we reveal that the Toll/NF-kappa B pathway is essential for wound closure in late Drosophila embryos. Toll mutants and Dif dorsal (NF-kappa B) double mutants are unable to repair epidermal gaps. Dorsal is activated on wounding, and Dif and Dorsal are required for the sustained down-regulation of E-cadherin, an obligatory component of the adherens junctions (AJs), at the wound edge. This remodeling of the AJs promotes the assembly of an actin-myosin cable at the wound margin; contraction of the actin cable, in turn, closes the wound. In the absence of Toll or Dif and dorsal (dl), both E-cadherin down-regulation and actin-cable formation fail, thus resulting in open epidermal gaps. Given the conservation of the Toll/NF-kappa B pathway in mammals and the epithelial expression of many components of the pathway, this function in wound healing is likely to be conserved in vertebrates. |
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The Toll/NF-kappa B signaling pathway is required for epidermal wound repair in DrosophilaToll pathwayPROTEINNF-kappa B transcription factorsDrosophilaEMBRYOIMMUNE-RESPONSEADHERENS JUNCTIONSDORSAL-VENTRAL PATTERNGRAINY-HEADepithelial wound repairE-CADHERINGENEPOLARITYE-cadherinGRADIENTDrosophilaE-cadherinEpithelial wound repairNF-κB transcription factorsToll pathwayThe Toll/NF-kappa B pathway, first identified in studies of dorsal-ventral polarity in the early Drosophila embryo, is well known for its role in the innate immune response. Here, we reveal that the Toll/NF-kappa B pathway is essential for wound closure in late Drosophila embryos. Toll mutants and Dif dorsal (NF-kappa B) double mutants are unable to repair epidermal gaps. Dorsal is activated on wounding, and Dif and Dorsal are required for the sustained down-regulation of E-cadherin, an obligatory component of the adherens junctions (AJs), at the wound edge. This remodeling of the AJs promotes the assembly of an actin-myosin cable at the wound margin; contraction of the actin cable, in turn, closes the wound. In the absence of Toll or Dif and dorsal (dl), both E-cadherin down-regulation and actin-cable formation fail, thus resulting in open epidermal gaps. Given the conservation of the Toll/NF-kappa B pathway in mammals and the epithelial expression of many components of the pathway, this function in wound healing is likely to be conserved in vertebrates.NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)Centro de Estudos de Doenças Crónicas (CEDOC)RUNCarvalho, LaraJacinto, AntónioMatova, Nina2017-10-18T22:00:45Z2014-12-162014-12-16T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10application/pdfhttp://hdl.handle.net/10362/24296eng0027-8424PURE: 319115https://doi.org/10.1073/pnas.1408224111info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:12:36Zoai:run.unl.pt:10362/24296Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:28:01.591273Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
The Toll/NF-kappa B signaling pathway is required for epidermal wound repair in Drosophila |
title |
The Toll/NF-kappa B signaling pathway is required for epidermal wound repair in Drosophila |
spellingShingle |
The Toll/NF-kappa B signaling pathway is required for epidermal wound repair in Drosophila Carvalho, Lara Toll pathway PROTEIN NF-kappa B transcription factors Drosophila EMBRYO IMMUNE-RESPONSE ADHERENS JUNCTIONS DORSAL-VENTRAL PATTERN GRAINY-HEAD epithelial wound repair E-CADHERIN GENE POLARITY E-cadherin GRADIENT Drosophila E-cadherin Epithelial wound repair NF-κB transcription factors Toll pathway |
title_short |
The Toll/NF-kappa B signaling pathway is required for epidermal wound repair in Drosophila |
title_full |
The Toll/NF-kappa B signaling pathway is required for epidermal wound repair in Drosophila |
title_fullStr |
The Toll/NF-kappa B signaling pathway is required for epidermal wound repair in Drosophila |
title_full_unstemmed |
The Toll/NF-kappa B signaling pathway is required for epidermal wound repair in Drosophila |
title_sort |
The Toll/NF-kappa B signaling pathway is required for epidermal wound repair in Drosophila |
author |
Carvalho, Lara |
author_facet |
Carvalho, Lara Jacinto, António Matova, Nina |
author_role |
author |
author2 |
Jacinto, António Matova, Nina |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM) Centro de Estudos de Doenças Crónicas (CEDOC) RUN |
dc.contributor.author.fl_str_mv |
Carvalho, Lara Jacinto, António Matova, Nina |
dc.subject.por.fl_str_mv |
Toll pathway PROTEIN NF-kappa B transcription factors Drosophila EMBRYO IMMUNE-RESPONSE ADHERENS JUNCTIONS DORSAL-VENTRAL PATTERN GRAINY-HEAD epithelial wound repair E-CADHERIN GENE POLARITY E-cadherin GRADIENT Drosophila E-cadherin Epithelial wound repair NF-κB transcription factors Toll pathway |
topic |
Toll pathway PROTEIN NF-kappa B transcription factors Drosophila EMBRYO IMMUNE-RESPONSE ADHERENS JUNCTIONS DORSAL-VENTRAL PATTERN GRAINY-HEAD epithelial wound repair E-CADHERIN GENE POLARITY E-cadherin GRADIENT Drosophila E-cadherin Epithelial wound repair NF-κB transcription factors Toll pathway |
description |
The Toll/NF-kappa B pathway, first identified in studies of dorsal-ventral polarity in the early Drosophila embryo, is well known for its role in the innate immune response. Here, we reveal that the Toll/NF-kappa B pathway is essential for wound closure in late Drosophila embryos. Toll mutants and Dif dorsal (NF-kappa B) double mutants are unable to repair epidermal gaps. Dorsal is activated on wounding, and Dif and Dorsal are required for the sustained down-regulation of E-cadherin, an obligatory component of the adherens junctions (AJs), at the wound edge. This remodeling of the AJs promotes the assembly of an actin-myosin cable at the wound margin; contraction of the actin cable, in turn, closes the wound. In the absence of Toll or Dif and dorsal (dl), both E-cadherin down-regulation and actin-cable formation fail, thus resulting in open epidermal gaps. Given the conservation of the Toll/NF-kappa B pathway in mammals and the epithelial expression of many components of the pathway, this function in wound healing is likely to be conserved in vertebrates. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-12-16 2014-12-16T00:00:00Z 2017-10-18T22:00:45Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/24296 |
url |
http://hdl.handle.net/10362/24296 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0027-8424 PURE: 319115 https://doi.org/10.1073/pnas.1408224111 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
10 application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799137907140722688 |