Fucoidan immobilized at the surface of a fibrous mesh presents toxic effects over melanoma cells, but not over non-cancer skin cells
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/66424 |
Resumo: | The use of fucoidan, a marine-origin bioactive polymer, is herein proposed as a component of an innovative and effective strategy against melanoma, one of the most aggressive skin cancers. First, fucoidan antitumor activity, in its soluble form, was assessed presenting increased cytotoxicity over melanoma cells when compared to human dermal fibroblasts and keratinocytes. After this antitumor activity validation and trying to develop a more targeted and local strategy aiming to diminish the cytotoxic effects over noncancer cells, fucoidan was immobilized at the surface of an electrospun nanofiber mesh (NFM_Fu), envisioning the development of a therapeutic patch. The maximum immobilization concentration was 1.2 mg mLâ 1, determined by the Toluidine Blue Assay and confirmed by XPS. Furthermore, NFM_Fu is more hydrophilic than NFM, presenting a contact angle of 36°, lower than the 121° of the control condition. NFM_Fu was able to reduce human melanoma cell viability by 50% without affecting human dermal fibroblasts and keratinocytes. Taken together, these results set the basis for a valuable approach for melanoma treatment. |
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Fucoidan immobilized at the surface of a fibrous mesh presents toxic effects over melanoma cells, but not over non-cancer skin cellsFucoidanMarine natural productsmelanomananofiber meshsurface functionalizationScience & TechnologyThe use of fucoidan, a marine-origin bioactive polymer, is herein proposed as a component of an innovative and effective strategy against melanoma, one of the most aggressive skin cancers. First, fucoidan antitumor activity, in its soluble form, was assessed presenting increased cytotoxicity over melanoma cells when compared to human dermal fibroblasts and keratinocytes. After this antitumor activity validation and trying to develop a more targeted and local strategy aiming to diminish the cytotoxic effects over noncancer cells, fucoidan was immobilized at the surface of an electrospun nanofiber mesh (NFM_Fu), envisioning the development of a therapeutic patch. The maximum immobilization concentration was 1.2 mg mLâ 1, determined by the Toluidine Blue Assay and confirmed by XPS. Furthermore, NFM_Fu is more hydrophilic than NFM, presenting a contact angle of 36°, lower than the 121° of the control condition. NFM_Fu was able to reduce human melanoma cell viability by 50% without affecting human dermal fibroblasts and keratinocytes. Taken together, these results set the basis for a valuable approach for melanoma treatment.This work was developed under the scope of the Structured Projects for R&D&I NORTE-01-0145-FEDER-000021 and NORTE-01-0145-FEDER-000023 supported by the Northern Portugal Regional Operational Programme (NORTE 2020),under the Portugal 2020 Partnership Agreement. The authors would like also to thank NORTE 2020 for financing the Ph.D.scholarship of C.O.“Norte-08-5369-000037”and the Portuguese Foundation for Science and Technology for the Investigator Grant of A.M. (IF/00376/2014). Dr. Luísa Rodrigues is acknowledged by the XPS analysis.ACS PublicationsUniversidade do MinhoOliveira, C.Soares, A. I.Neves, N. M.Reis, R. L.Marques, A. P.Tiago, S. H.Martins, A.2020-052020-05-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/66424engOliveira C., Soares A. I., Neves N. M., Reis R. L., Marques A. P., Tiago S. H., Martins A. Fucoidan immobilized at the surface of a fibrous mesh presents toxic effects over melanoma cells, but not over non-cancer skin cells, Biomacromolecules, Vol. 21, Issue 7, pp. 2745–2754, doi:10.1021/acs.biomac.0c00482, 20201525-779710.1021/acs.biomac.0c0048232421313https://www.sciencedirect.com/science/article/pii/S0144861719307015info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:43:12Zoai:repositorium.sdum.uminho.pt:1822/66424Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:40:37.396877Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Fucoidan immobilized at the surface of a fibrous mesh presents toxic effects over melanoma cells, but not over non-cancer skin cells |
title |
Fucoidan immobilized at the surface of a fibrous mesh presents toxic effects over melanoma cells, but not over non-cancer skin cells |
spellingShingle |
Fucoidan immobilized at the surface of a fibrous mesh presents toxic effects over melanoma cells, but not over non-cancer skin cells Oliveira, C. Fucoidan Marine natural products melanoma nanofiber mesh surface functionalization Science & Technology |
title_short |
Fucoidan immobilized at the surface of a fibrous mesh presents toxic effects over melanoma cells, but not over non-cancer skin cells |
title_full |
Fucoidan immobilized at the surface of a fibrous mesh presents toxic effects over melanoma cells, but not over non-cancer skin cells |
title_fullStr |
Fucoidan immobilized at the surface of a fibrous mesh presents toxic effects over melanoma cells, but not over non-cancer skin cells |
title_full_unstemmed |
Fucoidan immobilized at the surface of a fibrous mesh presents toxic effects over melanoma cells, but not over non-cancer skin cells |
title_sort |
Fucoidan immobilized at the surface of a fibrous mesh presents toxic effects over melanoma cells, but not over non-cancer skin cells |
author |
Oliveira, C. |
author_facet |
Oliveira, C. Soares, A. I. Neves, N. M. Reis, R. L. Marques, A. P. Tiago, S. H. Martins, A. |
author_role |
author |
author2 |
Soares, A. I. Neves, N. M. Reis, R. L. Marques, A. P. Tiago, S. H. Martins, A. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Oliveira, C. Soares, A. I. Neves, N. M. Reis, R. L. Marques, A. P. Tiago, S. H. Martins, A. |
dc.subject.por.fl_str_mv |
Fucoidan Marine natural products melanoma nanofiber mesh surface functionalization Science & Technology |
topic |
Fucoidan Marine natural products melanoma nanofiber mesh surface functionalization Science & Technology |
description |
The use of fucoidan, a marine-origin bioactive polymer, is herein proposed as a component of an innovative and effective strategy against melanoma, one of the most aggressive skin cancers. First, fucoidan antitumor activity, in its soluble form, was assessed presenting increased cytotoxicity over melanoma cells when compared to human dermal fibroblasts and keratinocytes. After this antitumor activity validation and trying to develop a more targeted and local strategy aiming to diminish the cytotoxic effects over noncancer cells, fucoidan was immobilized at the surface of an electrospun nanofiber mesh (NFM_Fu), envisioning the development of a therapeutic patch. The maximum immobilization concentration was 1.2 mg mLâ 1, determined by the Toluidine Blue Assay and confirmed by XPS. Furthermore, NFM_Fu is more hydrophilic than NFM, presenting a contact angle of 36°, lower than the 121° of the control condition. NFM_Fu was able to reduce human melanoma cell viability by 50% without affecting human dermal fibroblasts and keratinocytes. Taken together, these results set the basis for a valuable approach for melanoma treatment. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-05 2020-05-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/66424 |
url |
http://hdl.handle.net/1822/66424 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Oliveira C., Soares A. I., Neves N. M., Reis R. L., Marques A. P., Tiago S. H., Martins A. Fucoidan immobilized at the surface of a fibrous mesh presents toxic effects over melanoma cells, but not over non-cancer skin cells, Biomacromolecules, Vol. 21, Issue 7, pp. 2745–2754, doi:10.1021/acs.biomac.0c00482, 2020 1525-7797 10.1021/acs.biomac.0c00482 32421313 https://www.sciencedirect.com/science/article/pii/S0144861719307015 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
ACS Publications |
publisher.none.fl_str_mv |
ACS Publications |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132952137826304 |