Gemcitabine delivered by fucoidan/chitosan nanoparticles presents increased toxicity over human breast cancer cells

Detalhes bibliográficos
Autor(a) principal: Oliveira, Catarina
Data de Publicação: 2018
Outros Autores: Neves, N. M., Reis, R. L., Martins, A., Silva, T. H.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/58734
Resumo: Aim: To produce marine-origin nanoparticles (NPs) aiming to develop more effective and tolerated therapies for breast cancer. Materials & methods: NPs based in two marine-origin polymers (fucoidan and chitosan) were prepared by polyelectrolyte complexation, for the delivery of an antitumor drug model (gemcitabine [Gem]). Results: Final formulation resulted in stable NPs around 115â 140 nm in size and with a polydispersity index less than 0.2. Gem was encapsulated at a maximum entrapment efficiency of 35â 42%. Drug-release studies demonstrated that around 84% of Gem is released within 4 h. Cytotoxicity results of Gem-loaded NPs showed increased toxicity (around 25%) when compared with free Gem. Conclusion: The drug-loaded NPs present increased toxicity over human breast cancer cells without increasing toxic effects over endothelial cells.
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spelling Gemcitabine delivered by fucoidan/chitosan nanoparticles presents increased toxicity over human breast cancer cellsbreast cancer cellsChitosanDrug Delivery Systemendothelial cellsFucoidangemcitabineNanoparticlesPolyelectrolyte complexationScience & TechnologyAim: To produce marine-origin nanoparticles (NPs) aiming to develop more effective and tolerated therapies for breast cancer. Materials & methods: NPs based in two marine-origin polymers (fucoidan and chitosan) were prepared by polyelectrolyte complexation, for the delivery of an antitumor drug model (gemcitabine [Gem]). Results: Final formulation resulted in stable NPs around 115â 140 nm in size and with a polydispersity index less than 0.2. Gem was encapsulated at a maximum entrapment efficiency of 35â 42%. Drug-release studies demonstrated that around 84% of Gem is released within 4 h. Cytotoxicity results of Gem-loaded NPs showed increased toxicity (around 25%) when compared with free Gem. Conclusion: The drug-loaded NPs present increased toxicity over human breast cancer cells without increasing toxic effects over endothelial cells.The authors thank the PhD scholarship of C Oliveira for ‘NORTE-08-5369-000037’ financed by NORTE 2020, Portuguese Foundation for Science and Tecnology (FCT) for the investigator grant of A Martins for (IF/00376/2014) and the support from European Research Council under the Advanced Grant ComplexiTE. The work here reported also received financial support from the European Regional Development Fund (ERDF) under the Structured Project ‘Accelerating tissue engineering and personalized medicine discoveries by the integration of key enabling nanotechnologies, marine-derived biomaterials and stem cells’ (NORTE-01-0145FEDER-000021),supported by Norte Portugal Regional Operational Program(NORTE2020), under the PORTUGAL2020 Partnership Agreement. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. No writing assistance was utilized in the production of this manuscript.info:eu-repo/semantics/publishedVersionFuture Medicine LtdUniversidade do MinhoOliveira, CatarinaNeves, N. M.Reis, R. L.Martins, A.Silva, T. H.2018-092018-09-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/58734engOliveira C., Neves N. M., Reis R. L., Silva T. H., Martins A. Gemcitabine delivered by fucoidan/chitosan nanoparticles presents increased toxicity over human breast cancer cells, Nanomedicine, doi:10.2217/nnm-2018-0004, 20181743-588910.2217/nnm-2018-000430189774info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:29:33Zoai:repositorium.sdum.uminho.pt:1822/58734Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:24:33.910672Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Gemcitabine delivered by fucoidan/chitosan nanoparticles presents increased toxicity over human breast cancer cells
title Gemcitabine delivered by fucoidan/chitosan nanoparticles presents increased toxicity over human breast cancer cells
spellingShingle Gemcitabine delivered by fucoidan/chitosan nanoparticles presents increased toxicity over human breast cancer cells
Oliveira, Catarina
breast cancer cells
Chitosan
Drug Delivery System
endothelial cells
Fucoidan
gemcitabine
Nanoparticles
Polyelectrolyte complexation
Science & Technology
title_short Gemcitabine delivered by fucoidan/chitosan nanoparticles presents increased toxicity over human breast cancer cells
title_full Gemcitabine delivered by fucoidan/chitosan nanoparticles presents increased toxicity over human breast cancer cells
title_fullStr Gemcitabine delivered by fucoidan/chitosan nanoparticles presents increased toxicity over human breast cancer cells
title_full_unstemmed Gemcitabine delivered by fucoidan/chitosan nanoparticles presents increased toxicity over human breast cancer cells
title_sort Gemcitabine delivered by fucoidan/chitosan nanoparticles presents increased toxicity over human breast cancer cells
author Oliveira, Catarina
author_facet Oliveira, Catarina
Neves, N. M.
Reis, R. L.
Martins, A.
Silva, T. H.
author_role author
author2 Neves, N. M.
Reis, R. L.
Martins, A.
Silva, T. H.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Oliveira, Catarina
Neves, N. M.
Reis, R. L.
Martins, A.
Silva, T. H.
dc.subject.por.fl_str_mv breast cancer cells
Chitosan
Drug Delivery System
endothelial cells
Fucoidan
gemcitabine
Nanoparticles
Polyelectrolyte complexation
Science & Technology
topic breast cancer cells
Chitosan
Drug Delivery System
endothelial cells
Fucoidan
gemcitabine
Nanoparticles
Polyelectrolyte complexation
Science & Technology
description Aim: To produce marine-origin nanoparticles (NPs) aiming to develop more effective and tolerated therapies for breast cancer. Materials & methods: NPs based in two marine-origin polymers (fucoidan and chitosan) were prepared by polyelectrolyte complexation, for the delivery of an antitumor drug model (gemcitabine [Gem]). Results: Final formulation resulted in stable NPs around 115â 140 nm in size and with a polydispersity index less than 0.2. Gem was encapsulated at a maximum entrapment efficiency of 35â 42%. Drug-release studies demonstrated that around 84% of Gem is released within 4 h. Cytotoxicity results of Gem-loaded NPs showed increased toxicity (around 25%) when compared with free Gem. Conclusion: The drug-loaded NPs present increased toxicity over human breast cancer cells without increasing toxic effects over endothelial cells.
publishDate 2018
dc.date.none.fl_str_mv 2018-09
2018-09-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/58734
url http://hdl.handle.net/1822/58734
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Oliveira C., Neves N. M., Reis R. L., Silva T. H., Martins A. Gemcitabine delivered by fucoidan/chitosan nanoparticles presents increased toxicity over human breast cancer cells, Nanomedicine, doi:10.2217/nnm-2018-0004, 2018
1743-5889
10.2217/nnm-2018-0004
30189774
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Future Medicine Ltd
publisher.none.fl_str_mv Future Medicine Ltd
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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