Biochemical changes in cancer cells induced by photoactive nanosystem based on carbon dots loaded with Ru-complex
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.13/5234 |
Resumo: | Carbon dots (CDs) and N-carbon dots (N-CDs) loaded with Ru-complex (CDs@RuCN, N-CDs@RuCN, respec tively) were investigated as media imposing biochemical changes induced by UV illumination of ovarian cancer, A2780, and osteosarcoma, CAL72, cells. Synchrotron radiation-based Fourier Transform Infrared Spectroscopy was performed, and the spectra were subjected to a Principal Component Analysis. The CDs@RuCN and N CDs@RuCN effects on cancer cells were analyzed by the theoretical modelling of the stability of the composite systems and a protein database search. Moreover, a detailed evaluation of surface and optical properties of CDs@RuCN and N-CDs@RuCN was carried out. Results demonstrated selective action of the CDs@RuCN and N CDs@RuCN-based photodynamic therapy, with N-CDs@RuCN being the most active in inducing changes in A2780 and CDs@RuCN in CAL72 cells. We assume that different surface charges of nanoparticles led to direct interactions of N-CDs@RuCN with a Wnt signalling pathway in A2780 and those of CDs@RuCN with PI3–K/Akt in CAL72 cells and that further biochemical changes occurred upon light illumination. |
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Biochemical changes in cancer cells induced by photoactive nanosystem based on carbon dots loaded with Ru-complexAnticancer photodynamic therapyBiochemical changesN-doped carbon dotsCarbon dotsRu-metallodrug.Faculdade de Ciências Exatas e da EngenhariaCarbon dots (CDs) and N-carbon dots (N-CDs) loaded with Ru-complex (CDs@RuCN, N-CDs@RuCN, respec tively) were investigated as media imposing biochemical changes induced by UV illumination of ovarian cancer, A2780, and osteosarcoma, CAL72, cells. Synchrotron radiation-based Fourier Transform Infrared Spectroscopy was performed, and the spectra were subjected to a Principal Component Analysis. The CDs@RuCN and N CDs@RuCN effects on cancer cells were analyzed by the theoretical modelling of the stability of the composite systems and a protein database search. Moreover, a detailed evaluation of surface and optical properties of CDs@RuCN and N-CDs@RuCN was carried out. Results demonstrated selective action of the CDs@RuCN and N CDs@RuCN-based photodynamic therapy, with N-CDs@RuCN being the most active in inducing changes in A2780 and CDs@RuCN in CAL72 cells. We assume that different surface charges of nanoparticles led to direct interactions of N-CDs@RuCN with a Wnt signalling pathway in A2780 and those of CDs@RuCN with PI3–K/Akt in CAL72 cells and that further biochemical changes occurred upon light illumination.ElsevierDigitUMaNesic, Maja D.Ducic, TanjaGonçalves, MaraStepić, MilutinAlgarra, ManuelSoto, JuanGemović, BranislavaBandosz, Teresa J.Petkovic, Marijana2023-06-19T10:51:31Z20222022-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.13/5234engNešić, M. D., Dučić, T., Gonçalves, M., Stepić, M., Algarra, M., Soto, J., ... & Petković, M. (2022). Biochemical changes in cancer cells induced by photoactive nanosystem based on carbon dots loaded with Ru-complex. Chemico-Biological Interactions, 360, 109950.10.1016/j.cbi.2022.109950info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-06-25T03:30:38Zoai:digituma.uma.pt:10400.13/5234Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:01:32.358845Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Biochemical changes in cancer cells induced by photoactive nanosystem based on carbon dots loaded with Ru-complex |
title |
Biochemical changes in cancer cells induced by photoactive nanosystem based on carbon dots loaded with Ru-complex |
spellingShingle |
Biochemical changes in cancer cells induced by photoactive nanosystem based on carbon dots loaded with Ru-complex Nesic, Maja D. Anticancer photodynamic therapy Biochemical changes N-doped carbon dots Carbon dots Ru-metallodrug . Faculdade de Ciências Exatas e da Engenharia |
title_short |
Biochemical changes in cancer cells induced by photoactive nanosystem based on carbon dots loaded with Ru-complex |
title_full |
Biochemical changes in cancer cells induced by photoactive nanosystem based on carbon dots loaded with Ru-complex |
title_fullStr |
Biochemical changes in cancer cells induced by photoactive nanosystem based on carbon dots loaded with Ru-complex |
title_full_unstemmed |
Biochemical changes in cancer cells induced by photoactive nanosystem based on carbon dots loaded with Ru-complex |
title_sort |
Biochemical changes in cancer cells induced by photoactive nanosystem based on carbon dots loaded with Ru-complex |
author |
Nesic, Maja D. |
author_facet |
Nesic, Maja D. Ducic, Tanja Gonçalves, Mara Stepić, Milutin Algarra, Manuel Soto, Juan Gemović, Branislava Bandosz, Teresa J. Petkovic, Marijana |
author_role |
author |
author2 |
Ducic, Tanja Gonçalves, Mara Stepić, Milutin Algarra, Manuel Soto, Juan Gemović, Branislava Bandosz, Teresa J. Petkovic, Marijana |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
DigitUMa |
dc.contributor.author.fl_str_mv |
Nesic, Maja D. Ducic, Tanja Gonçalves, Mara Stepić, Milutin Algarra, Manuel Soto, Juan Gemović, Branislava Bandosz, Teresa J. Petkovic, Marijana |
dc.subject.por.fl_str_mv |
Anticancer photodynamic therapy Biochemical changes N-doped carbon dots Carbon dots Ru-metallodrug . Faculdade de Ciências Exatas e da Engenharia |
topic |
Anticancer photodynamic therapy Biochemical changes N-doped carbon dots Carbon dots Ru-metallodrug . Faculdade de Ciências Exatas e da Engenharia |
description |
Carbon dots (CDs) and N-carbon dots (N-CDs) loaded with Ru-complex (CDs@RuCN, N-CDs@RuCN, respec tively) were investigated as media imposing biochemical changes induced by UV illumination of ovarian cancer, A2780, and osteosarcoma, CAL72, cells. Synchrotron radiation-based Fourier Transform Infrared Spectroscopy was performed, and the spectra were subjected to a Principal Component Analysis. The CDs@RuCN and N CDs@RuCN effects on cancer cells were analyzed by the theoretical modelling of the stability of the composite systems and a protein database search. Moreover, a detailed evaluation of surface and optical properties of CDs@RuCN and N-CDs@RuCN was carried out. Results demonstrated selective action of the CDs@RuCN and N CDs@RuCN-based photodynamic therapy, with N-CDs@RuCN being the most active in inducing changes in A2780 and CDs@RuCN in CAL72 cells. We assume that different surface charges of nanoparticles led to direct interactions of N-CDs@RuCN with a Wnt signalling pathway in A2780 and those of CDs@RuCN with PI3–K/Akt in CAL72 cells and that further biochemical changes occurred upon light illumination. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022 2022-01-01T00:00:00Z 2023-06-19T10:51:31Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.13/5234 |
url |
http://hdl.handle.net/10400.13/5234 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Nešić, M. D., Dučić, T., Gonçalves, M., Stepić, M., Algarra, M., Soto, J., ... & Petković, M. (2022). Biochemical changes in cancer cells induced by photoactive nanosystem based on carbon dots loaded with Ru-complex. Chemico-Biological Interactions, 360, 109950. 10.1016/j.cbi.2022.109950 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799131683102916608 |