Hybrid nanosystems based on natural polymers as protein carriers for respiratory delivery: stability and toxicological evaluation

Detalhes bibliográficos
Autor(a) principal: Rodrigues, Susana
Data de Publicação: 2015
Outros Autores: Cordeiro, Clara, Seijo, Begona, Remunan-Lopez, Carmen, Grenha, Ana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.1/11539
Resumo: Chitosan/carrageenan/tripolyphosphate nanoparticles were previously presented as holding potential for an application in transmucosal delivery of macromolecules, with tripolyphosphate demonstrating to contribute for both size reduction and stabilisation of the nanoparticles. This work was aimed at evaluating the capacity of the nanoparticles as protein carriers for pulmonary and nasal transmucosal delivery, further assessing their biocompatibility pattern regarding that application. Nanoparticles demonstrated stability in presence of lysozyme, while freeze-drying was shown to preserve their characteristics when glucose or sucrose were used as cryoprotectants. Bovine serum albumin was associated to the nanoparticles, which were successfully microencapsulated by spray-drying to meet the aerodynamic requirements inherent to pulmonary delivery. Finally, a satisfactory biocompatibility profile was demonstrated upon exposure of two respiratory cell lines (Calu-3 and A549 cells) to the carriers. A negligible effect on cell viability along with no alterations on transepithelial electrical resistance and no induction of inflammatory response were observed. (C) 2015 Elsevier Ltd. All rights reserved.
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spelling Hybrid nanosystems based on natural polymers as protein carriers for respiratory delivery: stability and toxicological evaluationMicroencapsulated chitosan nanoparticlesIn-vitro evaluationDrug-deliveryCell-cultureChitosan/carrageenan nanoparticlesTransmucosal deliverySilica nanoparticlesPulmonary deliveryChemical-stabilityPlga nanoparticlesChitosan/carrageenan/tripolyphosphate nanoparticles were previously presented as holding potential for an application in transmucosal delivery of macromolecules, with tripolyphosphate demonstrating to contribute for both size reduction and stabilisation of the nanoparticles. This work was aimed at evaluating the capacity of the nanoparticles as protein carriers for pulmonary and nasal transmucosal delivery, further assessing their biocompatibility pattern regarding that application. Nanoparticles demonstrated stability in presence of lysozyme, while freeze-drying was shown to preserve their characteristics when glucose or sucrose were used as cryoprotectants. Bovine serum albumin was associated to the nanoparticles, which were successfully microencapsulated by spray-drying to meet the aerodynamic requirements inherent to pulmonary delivery. Finally, a satisfactory biocompatibility profile was demonstrated upon exposure of two respiratory cell lines (Calu-3 and A549 cells) to the carriers. A negligible effect on cell viability along with no alterations on transepithelial electrical resistance and no induction of inflammatory response were observed. (C) 2015 Elsevier Ltd. All rights reserved.National Portuguese funding through FCT - Fundacao para a Ciencia e a Tecnologia [PTDC/SAU-FCF/100291/2008, PTDC/DTP-FTO/0094/2012, PEst-OE/EQB/LA0023/2013]; Spanish Government (ISCIII, Accion Estrategica de Salud) [PS09/00816]; FCT [SFRH/BD/52426/2013]; ShareBiotech mobility grant; FCT project [PEst-OE/MAT/UI0006/2014]Elsevier Sci LtdSapientiaRodrigues, SusanaCordeiro, ClaraSeijo, BegonaRemunan-Lopez, CarmenGrenha, Ana2018-12-07T14:53:29Z2015-062015-06-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/11539eng0144-861710.1016/j.carbpol.2015.01.048info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:23:22Zoai:sapientia.ualg.pt:10400.1/11539Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:03:01.990280Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Hybrid nanosystems based on natural polymers as protein carriers for respiratory delivery: stability and toxicological evaluation
title Hybrid nanosystems based on natural polymers as protein carriers for respiratory delivery: stability and toxicological evaluation
spellingShingle Hybrid nanosystems based on natural polymers as protein carriers for respiratory delivery: stability and toxicological evaluation
Rodrigues, Susana
Microencapsulated chitosan nanoparticles
In-vitro evaluation
Drug-delivery
Cell-culture
Chitosan/carrageenan nanoparticles
Transmucosal delivery
Silica nanoparticles
Pulmonary delivery
Chemical-stability
Plga nanoparticles
title_short Hybrid nanosystems based on natural polymers as protein carriers for respiratory delivery: stability and toxicological evaluation
title_full Hybrid nanosystems based on natural polymers as protein carriers for respiratory delivery: stability and toxicological evaluation
title_fullStr Hybrid nanosystems based on natural polymers as protein carriers for respiratory delivery: stability and toxicological evaluation
title_full_unstemmed Hybrid nanosystems based on natural polymers as protein carriers for respiratory delivery: stability and toxicological evaluation
title_sort Hybrid nanosystems based on natural polymers as protein carriers for respiratory delivery: stability and toxicological evaluation
author Rodrigues, Susana
author_facet Rodrigues, Susana
Cordeiro, Clara
Seijo, Begona
Remunan-Lopez, Carmen
Grenha, Ana
author_role author
author2 Cordeiro, Clara
Seijo, Begona
Remunan-Lopez, Carmen
Grenha, Ana
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Sapientia
dc.contributor.author.fl_str_mv Rodrigues, Susana
Cordeiro, Clara
Seijo, Begona
Remunan-Lopez, Carmen
Grenha, Ana
dc.subject.por.fl_str_mv Microencapsulated chitosan nanoparticles
In-vitro evaluation
Drug-delivery
Cell-culture
Chitosan/carrageenan nanoparticles
Transmucosal delivery
Silica nanoparticles
Pulmonary delivery
Chemical-stability
Plga nanoparticles
topic Microencapsulated chitosan nanoparticles
In-vitro evaluation
Drug-delivery
Cell-culture
Chitosan/carrageenan nanoparticles
Transmucosal delivery
Silica nanoparticles
Pulmonary delivery
Chemical-stability
Plga nanoparticles
description Chitosan/carrageenan/tripolyphosphate nanoparticles were previously presented as holding potential for an application in transmucosal delivery of macromolecules, with tripolyphosphate demonstrating to contribute for both size reduction and stabilisation of the nanoparticles. This work was aimed at evaluating the capacity of the nanoparticles as protein carriers for pulmonary and nasal transmucosal delivery, further assessing their biocompatibility pattern regarding that application. Nanoparticles demonstrated stability in presence of lysozyme, while freeze-drying was shown to preserve their characteristics when glucose or sucrose were used as cryoprotectants. Bovine serum albumin was associated to the nanoparticles, which were successfully microencapsulated by spray-drying to meet the aerodynamic requirements inherent to pulmonary delivery. Finally, a satisfactory biocompatibility profile was demonstrated upon exposure of two respiratory cell lines (Calu-3 and A549 cells) to the carriers. A negligible effect on cell viability along with no alterations on transepithelial electrical resistance and no induction of inflammatory response were observed. (C) 2015 Elsevier Ltd. All rights reserved.
publishDate 2015
dc.date.none.fl_str_mv 2015-06
2015-06-01T00:00:00Z
2018-12-07T14:53:29Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.1/11539
url http://hdl.handle.net/10400.1/11539
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0144-8617
10.1016/j.carbpol.2015.01.048
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier Sci Ltd
publisher.none.fl_str_mv Elsevier Sci Ltd
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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