Colonisation of the proximal intestinal remnant in newborn infants with enterostomy
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2019 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | https://doi.org/10.1136/bmjopen-2019-028916 |
Resumo: | This project was supported by ERDF through the operation POCI01–0145-FEDER-007746 funded by the Programa Operacional Competitividade e Internacionalização—COMPETE2020 and by National Funds through FCT— Fundação para a Ciência e a Tecnologia within CINTESIS, R&D Unit (reference UID/ IC/4255/2013). |
| id |
RCAP_481447b532dfab160e4cb66a67b8282d |
|---|---|
| oai_identifier_str |
oai:run.unl.pt:10362/89434 |
| network_acronym_str |
RCAP |
| network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| repository_id_str |
7160 |
| spelling |
Colonisation of the proximal intestinal remnant in newborn infants with enterostomya longitudinal study protocolcongenital malformations of the gastrointestinal tractdysbiosisenterostomymicrobiotanecrotizing enterocolitisnewborn infantsMedicine(all)This project was supported by ERDF through the operation POCI01–0145-FEDER-007746 funded by the Programa Operacional Competitividade e Internacionalização—COMPETE2020 and by National Funds through FCT— Fundação para a Ciência e a Tecnologia within CINTESIS, R&D Unit (reference UID/ IC/4255/2013).INTRODUCTION: The gut microbiota plays a main role in the maintenance of host's health. Exposure to different conditions in early life contributes to distinct 'pioneer' bacterial communities in the intestine, which shape the newborn infant development. Newborn infants with congenital malformations of the gastrointestinal tract (CMGIT), necrotising enterocolitis (NEC) and spontaneous intestinal perforation (SIP) commonly require abdominal surgery and enterostomy. The knowledge about the colonisation of these newborns' intestine by microorganisms is scarce. This protocol is designed to explore the microbial colonisation over time of the proximal intestinal remnant in newborn infants who underwent surgery for CMGIT, NEC or SIP and require enterostomy. METHODS AND ANALYSIS: The literature about microbiota colonisation in newborn infants with enterostomy was reviewed and an observational, longitudinal, prospective study was designed. The infants will be recruited at the Neonatal Intensive Care Unit of the Hospital Dona Estefânia, Centro Hospitalar Universitário de Lisboa Central. Samples of the enterostomy effluent will be collected every 3 days, through 21 days after the first collection. The microorganisms colonising the proximal intestinal remnant will be identified using the 16S rRNA sequence analysis and a subset of microorganisms will be quantified using real-time PCR. This protocol may serve as basis for future observational and interventional studies on the modulation of the intestinal microbiota (eg, probiotics) on short and long-term outcomes in this population. ETHICS AND DISSEMINATION: This study protocol was approved by the Ethics Committee of Centro Hospitalar Universitário de Lisboa Central (441/2017) and by the Ethics Committee of NOVA Medical School, Universidade Nova de Lisboa (n°50/2018/CEFCM). The results will be spread through peer-reviewed publications and presentations at international scientific meetings. TRIAL REGISTRATION NUMBER: NCT03340259.NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)RUNBarreiros Mota, InêsMarques, CláudiaFaria, AnaNeto, Maria TeresaCordeiro-Ferreira, GonçaloVirella, DanielPita, AnaPereira-da-Silva, LuísCalhau, Conceição2019-12-05T23:22:17Z2019-11-242019-11-24T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://doi.org/10.1136/bmjopen-2019-028916eng2044-6055PURE: 15702430http://www.scopus.com/inward/record.url?scp=85075569511&partnerID=8YFLogxKhttps://doi.org/10.1136/bmjopen-2019-028916info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:39:46Zoai:run.unl.pt:10362/89434Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:36:59.707638Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Colonisation of the proximal intestinal remnant in newborn infants with enterostomy a longitudinal study protocol |
| title |
Colonisation of the proximal intestinal remnant in newborn infants with enterostomy |
| spellingShingle |
Colonisation of the proximal intestinal remnant in newborn infants with enterostomy Barreiros Mota, Inês congenital malformations of the gastrointestinal tract dysbiosis enterostomy microbiota necrotizing enterocolitis newborn infants Medicine(all) |
| title_short |
Colonisation of the proximal intestinal remnant in newborn infants with enterostomy |
| title_full |
Colonisation of the proximal intestinal remnant in newborn infants with enterostomy |
| title_fullStr |
Colonisation of the proximal intestinal remnant in newborn infants with enterostomy |
| title_full_unstemmed |
Colonisation of the proximal intestinal remnant in newborn infants with enterostomy |
| title_sort |
Colonisation of the proximal intestinal remnant in newborn infants with enterostomy |
| author |
Barreiros Mota, Inês |
| author_facet |
Barreiros Mota, Inês Marques, Cláudia Faria, Ana Neto, Maria Teresa Cordeiro-Ferreira, Gonçalo Virella, Daniel Pita, Ana Pereira-da-Silva, Luís Calhau, Conceição |
| author_role |
author |
| author2 |
Marques, Cláudia Faria, Ana Neto, Maria Teresa Cordeiro-Ferreira, Gonçalo Virella, Daniel Pita, Ana Pereira-da-Silva, Luís Calhau, Conceição |
| author2_role |
author author author author author author author author |
| dc.contributor.none.fl_str_mv |
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM) RUN |
| dc.contributor.author.fl_str_mv |
Barreiros Mota, Inês Marques, Cláudia Faria, Ana Neto, Maria Teresa Cordeiro-Ferreira, Gonçalo Virella, Daniel Pita, Ana Pereira-da-Silva, Luís Calhau, Conceição |
| dc.subject.por.fl_str_mv |
congenital malformations of the gastrointestinal tract dysbiosis enterostomy microbiota necrotizing enterocolitis newborn infants Medicine(all) |
| topic |
congenital malformations of the gastrointestinal tract dysbiosis enterostomy microbiota necrotizing enterocolitis newborn infants Medicine(all) |
| description |
This project was supported by ERDF through the operation POCI01–0145-FEDER-007746 funded by the Programa Operacional Competitividade e Internacionalização—COMPETE2020 and by National Funds through FCT— Fundação para a Ciência e a Tecnologia within CINTESIS, R&D Unit (reference UID/ IC/4255/2013). |
| publishDate |
2019 |
| dc.date.none.fl_str_mv |
2019-12-05T23:22:17Z 2019-11-24 2019-11-24T00:00:00Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
https://doi.org/10.1136/bmjopen-2019-028916 |
| url |
https://doi.org/10.1136/bmjopen-2019-028916 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
2044-6055 PURE: 15702430 http://www.scopus.com/inward/record.url?scp=85075569511&partnerID=8YFLogxK https://doi.org/10.1136/bmjopen-2019-028916 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
| instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
| instacron_str |
RCAAP |
| institution |
RCAAP |
| reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
| repository.mail.fl_str_mv |
|
| _version_ |
1799137986889121792 |