Changes in Microbiota Profile in the Proximal Remnant Intestine in Infants Undergoing Surgery Requiring Enterostomy
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2023 |
| Outros Autores: | , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10362/160765 |
Resumo: | Funding Information: This project was supported by ERDF through the operation POCI01–0145-FEDER-007746 funded by the Programa Operacional Competitividade e Internacionalização—COMPETE2020 and by National Funds through FCT—Fundação para a Ciência e a Tecnologia, IP national support through CINTESIS, R&D Unit (UIDB/4255/2020 and UIDP/4255/2020), CHRC (UIDP/04923/2020 and UIDB/04923/2020). Publisher Copyright: © 2023 by the authors. |
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oai:run.unl.pt:10362/160765 |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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7160 |
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Changes in Microbiota Profile in the Proximal Remnant Intestine in Infants Undergoing Surgery Requiring Enterostomyenterostomyinfantsmicrobiotanecrotizing enterocolitisprematurityMicrobiologyMicrobiology (medical)VirologyFunding Information: This project was supported by ERDF through the operation POCI01–0145-FEDER-007746 funded by the Programa Operacional Competitividade e Internacionalização—COMPETE2020 and by National Funds through FCT—Fundação para a Ciência e a Tecnologia, IP national support through CINTESIS, R&D Unit (UIDB/4255/2020 and UIDP/4255/2020), CHRC (UIDP/04923/2020 and UIDB/04923/2020). Publisher Copyright: © 2023 by the authors.Early-life gut dysbiosis has been associated with an increased risk of inflammatory, metabolic, and immune diseases later in life. Data on gut microbiota changes in infants undergoing intestinal surgery requiring enterostomy are scarce. This prospective cohort study examined the enterostomy effluent of 29 infants who underwent intestinal surgery due to congenital malformations of the gastrointestinal tract, necrotizing enterocolitis, or spontaneous intestinal perforation. Initial effluent samples were collected immediately after surgery and final effluent samples were collected three weeks later. Gut microbiota composition was analysed using real-time PCR and 16S rRNA gene sequencing. Three weeks after surgery, an increase in total bacteria number (+21%, p = 0.026), a decrease in Staphylococcus (−21%, p = 0.002) and Candida spp. (−16%, p = 0.045), and an increase in Lactobacillus (+3%, p = 0.045) and in less abundant genera belonging to the Enterobacteriales family were found. An increase in alpha diversity (Shannon’s and Simpson’s indexes) and significant alterations in beta diversity were observed. A correlation of necrotizing enterocolitis with higher Staphylococcus abundance and higher alpha diversity was also observed. H2-blockers and/or proton pump inhibitor therapy were positively correlated with a higher total bacteria number. In conclusion, these results suggest that positive changes occur in the gut microbiota profile of infants three weeks after intestinal surgery.NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)Comprehensive Health Research Centre (CHRC) - pólo NMSCentro de Investigação em Tecnologias e Serviços de Saúde (CINTESIS)RUNBarreiros-Mota, InêsR. Araújo, JoãoMarques, CláudiaSousa, LauraMorais, JulianaCastela, InêsFaria, AnaNeto, Maria TeresaCordeiro-Ferreira, GonçaloVirella, DanielPita, AnaPereira-da-Silva, LuísCalhau, Conceição2023-11-30T22:19:57Z2023-102023-10-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/160765eng2076-2607PURE: 75293223https://doi.org/10.3390/microorganisms11102482info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-22T18:16:21Zoai:run.unl.pt:10362/160765Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-22T18:16:21Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Changes in Microbiota Profile in the Proximal Remnant Intestine in Infants Undergoing Surgery Requiring Enterostomy |
| title |
Changes in Microbiota Profile in the Proximal Remnant Intestine in Infants Undergoing Surgery Requiring Enterostomy |
| spellingShingle |
Changes in Microbiota Profile in the Proximal Remnant Intestine in Infants Undergoing Surgery Requiring Enterostomy Barreiros-Mota, Inês enterostomy infants microbiota necrotizing enterocolitis prematurity Microbiology Microbiology (medical) Virology |
| title_short |
Changes in Microbiota Profile in the Proximal Remnant Intestine in Infants Undergoing Surgery Requiring Enterostomy |
| title_full |
Changes in Microbiota Profile in the Proximal Remnant Intestine in Infants Undergoing Surgery Requiring Enterostomy |
| title_fullStr |
Changes in Microbiota Profile in the Proximal Remnant Intestine in Infants Undergoing Surgery Requiring Enterostomy |
| title_full_unstemmed |
Changes in Microbiota Profile in the Proximal Remnant Intestine in Infants Undergoing Surgery Requiring Enterostomy |
| title_sort |
Changes in Microbiota Profile in the Proximal Remnant Intestine in Infants Undergoing Surgery Requiring Enterostomy |
| author |
Barreiros-Mota, Inês |
| author_facet |
Barreiros-Mota, Inês R. Araújo, João Marques, Cláudia Sousa, Laura Morais, Juliana Castela, Inês Faria, Ana Neto, Maria Teresa Cordeiro-Ferreira, Gonçalo Virella, Daniel Pita, Ana Pereira-da-Silva, Luís Calhau, Conceição |
| author_role |
author |
| author2 |
R. Araújo, João Marques, Cláudia Sousa, Laura Morais, Juliana Castela, Inês Faria, Ana Neto, Maria Teresa Cordeiro-Ferreira, Gonçalo Virella, Daniel Pita, Ana Pereira-da-Silva, Luís Calhau, Conceição |
| author2_role |
author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM) Comprehensive Health Research Centre (CHRC) - pólo NMS Centro de Investigação em Tecnologias e Serviços de Saúde (CINTESIS) RUN |
| dc.contributor.author.fl_str_mv |
Barreiros-Mota, Inês R. Araújo, João Marques, Cláudia Sousa, Laura Morais, Juliana Castela, Inês Faria, Ana Neto, Maria Teresa Cordeiro-Ferreira, Gonçalo Virella, Daniel Pita, Ana Pereira-da-Silva, Luís Calhau, Conceição |
| dc.subject.por.fl_str_mv |
enterostomy infants microbiota necrotizing enterocolitis prematurity Microbiology Microbiology (medical) Virology |
| topic |
enterostomy infants microbiota necrotizing enterocolitis prematurity Microbiology Microbiology (medical) Virology |
| description |
Funding Information: This project was supported by ERDF through the operation POCI01–0145-FEDER-007746 funded by the Programa Operacional Competitividade e Internacionalização—COMPETE2020 and by National Funds through FCT—Fundação para a Ciência e a Tecnologia, IP national support through CINTESIS, R&D Unit (UIDB/4255/2020 and UIDP/4255/2020), CHRC (UIDP/04923/2020 and UIDB/04923/2020). Publisher Copyright: © 2023 by the authors. |
| publishDate |
2023 |
| dc.date.none.fl_str_mv |
2023-11-30T22:19:57Z 2023-10 2023-10-01T00:00:00Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/160765 |
| url |
http://hdl.handle.net/10362/160765 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
2076-2607 PURE: 75293223 https://doi.org/10.3390/microorganisms11102482 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
| instacron_str |
RCAAP |
| institution |
RCAAP |
| reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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mluisa.alvim@gmail.com |
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1817545972048199680 |