Leukemia inhibitory factor in rat fetal lung development: expression and functional studies

Detalhes bibliográficos
Autor(a) principal: Silva, Cristina Nogueira
Data de Publicação: 2012
Outros Autores: Piairo, Paulina C., Dias, Emanuel Carvalho, Peixoto, Francisca, Moura, Rute S., Pinto, Jorge Correia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/23972
Resumo: Conceived and designed the experiments: CN-S EC-D JC-P. Performed the experiments: CN-S PP EC-D FOP RSM. Analyzed the data: CN-S PP JC-P. Wrote the paper: CN-S PP RSM JC-P.
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spelling Leukemia inhibitory factor in rat fetal lung development: expression and functional studiesScience & TechnologyConceived and designed the experiments: CN-S EC-D JC-P. Performed the experiments: CN-S PP EC-D FOP RSM. Analyzed the data: CN-S PP JC-P. Wrote the paper: CN-S PP RSM JC-P.Leukemia inhibitory factor (LIF) and interleukin-6 (IL-6) are members of the family of the glycoprotein 130 (gp130)-type cytokines. These cytokines share gp130 as a common signal transducer, which explains why they show some functional redundancy. Recently, it was demonstrated that IL-6 promotes fetal lung branching. Additionally, LIF has been implicated in developmental processes of some branching organs. Thus, in this study LIF expression pattern and its effects on fetal rat lung morphogenesis were assessed. Methodology/Principal Findings LIF and its subunit receptor LIFRα expression levels were evaluated by immunohistochemistry and western blot in fetal rat lungs of different gestational ages, ranging from 13.5 to 21.5 days post-conception. Throughout all gestational ages studied, LIF was constitutively expressed in pulmonary epithelium, whereas LIFRα was first mainly expressed in the mesenchyme, but after pseudoglandular stage it was also observed in epithelial cells. These results point to a LIF epithelium-mesenchyme cross-talk, which is known to be important for lung branching process. Regarding functional studies, fetal lung explants were cultured with increasing doses of LIF or LIF neutralizing antibodies during 4 days. MAPK, AKT, and STAT3 phosphorylation in the treated lung explants was analyzed. LIF supplementation significantly inhibited lung growth in spite of an increase in p44/42 phosphorylation. On the other hand, LIF inhibition significantly stimulated lung growth via p38 and Akt pathways. Conclusions/Significance The present study describes that LIF and its subunit receptor LIFRα are constitutively expressed during fetal lung development and that they have an inhibitory physiological role on fetal lung branching.This project was funded by Fundacao para a Ciencia e a Tecnologia (PTDC/SAU-OBD/108051/2008) and by Seccao de Neonatologia da Sociedade Portuguesa de Pediatria (Grant ZERU 2008). RSM was supported by Fundacao para a Ciencia e a Tecnologia (reference SFRH/BPD/15408/2005). FOP was supported by Fundacao para a Ciencia e a Tecnologia (reference UMINHO/BII/172/2009). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Public Library of ScienceUniversidade do MinhoSilva, Cristina NogueiraPiairo, Paulina C.Dias, Emanuel CarvalhoPeixoto, FranciscaMoura, Rute S.Pinto, Jorge Correia2012-012012-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/23972eng1932-620310.1371/journal.pone.003051722291973http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0030517info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T11:58:09Zoai:repositorium.sdum.uminho.pt:1822/23972Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:47:50.780255Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Leukemia inhibitory factor in rat fetal lung development: expression and functional studies
title Leukemia inhibitory factor in rat fetal lung development: expression and functional studies
spellingShingle Leukemia inhibitory factor in rat fetal lung development: expression and functional studies
Silva, Cristina Nogueira
Science & Technology
title_short Leukemia inhibitory factor in rat fetal lung development: expression and functional studies
title_full Leukemia inhibitory factor in rat fetal lung development: expression and functional studies
title_fullStr Leukemia inhibitory factor in rat fetal lung development: expression and functional studies
title_full_unstemmed Leukemia inhibitory factor in rat fetal lung development: expression and functional studies
title_sort Leukemia inhibitory factor in rat fetal lung development: expression and functional studies
author Silva, Cristina Nogueira
author_facet Silva, Cristina Nogueira
Piairo, Paulina C.
Dias, Emanuel Carvalho
Peixoto, Francisca
Moura, Rute S.
Pinto, Jorge Correia
author_role author
author2 Piairo, Paulina C.
Dias, Emanuel Carvalho
Peixoto, Francisca
Moura, Rute S.
Pinto, Jorge Correia
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Silva, Cristina Nogueira
Piairo, Paulina C.
Dias, Emanuel Carvalho
Peixoto, Francisca
Moura, Rute S.
Pinto, Jorge Correia
dc.subject.por.fl_str_mv Science & Technology
topic Science & Technology
description Conceived and designed the experiments: CN-S EC-D JC-P. Performed the experiments: CN-S PP EC-D FOP RSM. Analyzed the data: CN-S PP JC-P. Wrote the paper: CN-S PP RSM JC-P.
publishDate 2012
dc.date.none.fl_str_mv 2012-01
2012-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/23972
url http://hdl.handle.net/1822/23972
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1932-6203
10.1371/journal.pone.0030517
22291973
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0030517
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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