The role of glycoprotein 130 family of cytokines in fetal rat lung development

Detalhes bibliográficos
Autor(a) principal: Silva, Cristina Nogueira
Data de Publicação: 2013
Outros Autores: Piairo, Paulina C., Dias, Emanuel Carvalho, Veiga, Carla, Moura, Rute S., Pinto, Jorge Correia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/24799
Resumo: The glycoprotein 130 (gp130) dependent family of cytokines comprises interleukin-6 (IL-6), IL-11, leukemia inhibitory factor (LIF), cardiotrophin-like cytokine (CLC), ciliary neurotrophic factor (CNTF), cardiotrophin-1 (CT-1), and oncostatin M (OSM). These cytokines share the membrane gp130 as a common signal transducer. Recently, it was demonstrated that IL-6 promotes, whereas LIF inhibits fetal lung branching. Thus, in this study, the effects on fetal lung morphogenesis of the other classical members of the gp130-type cytokines (IL-11, CLC, CNTF, CT-1 and OSM) were investigated. We also provide the first description of these cytokines and their common gp130 receptor protein expression patterns during rat lung development. Fetal rat lung explants were cultured in vitro with increasing concentrations of IL-11, CLC, CNTF, CT-1 and OSM. Treated lung explants were morphometrically analyzed and assessed for MAPK, PI3K/AKT and STAT3 signaling modifications. IL-11, which similarly to IL-6 acts through a gp130 homodimer receptor, significantly stimulated lung growth via p38 phosphorylation. On the other hand, CLC, CNTF, CT-1 and OSM, whose receptors are gp130 heterodimers, inhibited lung growth acting in different signal-transducing pathways. Thus, the present study demonstrated that although cytokines of the gp130 family share a common signal transducer, there are specific biological activities for each cytokine on lung development. Indeed, cytokine signaling through gp130 homodimers stimulate, whereas cytokine signaling through gp130 heterodimers inhibit lung branching.
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spelling The role of glycoprotein 130 family of cytokines in fetal rat lung developmentScience & TechnologyThe glycoprotein 130 (gp130) dependent family of cytokines comprises interleukin-6 (IL-6), IL-11, leukemia inhibitory factor (LIF), cardiotrophin-like cytokine (CLC), ciliary neurotrophic factor (CNTF), cardiotrophin-1 (CT-1), and oncostatin M (OSM). These cytokines share the membrane gp130 as a common signal transducer. Recently, it was demonstrated that IL-6 promotes, whereas LIF inhibits fetal lung branching. Thus, in this study, the effects on fetal lung morphogenesis of the other classical members of the gp130-type cytokines (IL-11, CLC, CNTF, CT-1 and OSM) were investigated. We also provide the first description of these cytokines and their common gp130 receptor protein expression patterns during rat lung development. Fetal rat lung explants were cultured in vitro with increasing concentrations of IL-11, CLC, CNTF, CT-1 and OSM. Treated lung explants were morphometrically analyzed and assessed for MAPK, PI3K/AKT and STAT3 signaling modifications. IL-11, which similarly to IL-6 acts through a gp130 homodimer receptor, significantly stimulated lung growth via p38 phosphorylation. On the other hand, CLC, CNTF, CT-1 and OSM, whose receptors are gp130 heterodimers, inhibited lung growth acting in different signal-transducing pathways. Thus, the present study demonstrated that although cytokines of the gp130 family share a common signal transducer, there are specific biological activities for each cytokine on lung development. Indeed, cytokine signaling through gp130 homodimers stimulate, whereas cytokine signaling through gp130 heterodimers inhibit lung branching.This project was funded by Fundacao para a Ciencia e a Tecnologia (PTDC/SAU-OBD/108051/2008) and by Seccao de Neonatologia da Sociedade Portuguesa de Pediatria (Grant ZERU 2008). PP was supported by Fundacao para a Ciencia e a Tecnologia (reference SFRH/BD/33410/2008). RSM was supported by Fundacao para a Ciencia e a Tecnologia (reference SFRH/BPD/15408/2005). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Public Library of ScienceUniversidade do MinhoSilva, Cristina NogueiraPiairo, Paulina C.Dias, Emanuel CarvalhoVeiga, CarlaMoura, Rute S.Pinto, Jorge Correia2013-062013-06-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/24799eng1932-620310.1371/journal.pone.006760723826327http://www.plosone.orginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:09:06Zoai:repositorium.sdum.uminho.pt:1822/24799Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:00:27.349600Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv The role of glycoprotein 130 family of cytokines in fetal rat lung development
title The role of glycoprotein 130 family of cytokines in fetal rat lung development
spellingShingle The role of glycoprotein 130 family of cytokines in fetal rat lung development
Silva, Cristina Nogueira
Science & Technology
title_short The role of glycoprotein 130 family of cytokines in fetal rat lung development
title_full The role of glycoprotein 130 family of cytokines in fetal rat lung development
title_fullStr The role of glycoprotein 130 family of cytokines in fetal rat lung development
title_full_unstemmed The role of glycoprotein 130 family of cytokines in fetal rat lung development
title_sort The role of glycoprotein 130 family of cytokines in fetal rat lung development
author Silva, Cristina Nogueira
author_facet Silva, Cristina Nogueira
Piairo, Paulina C.
Dias, Emanuel Carvalho
Veiga, Carla
Moura, Rute S.
Pinto, Jorge Correia
author_role author
author2 Piairo, Paulina C.
Dias, Emanuel Carvalho
Veiga, Carla
Moura, Rute S.
Pinto, Jorge Correia
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Silva, Cristina Nogueira
Piairo, Paulina C.
Dias, Emanuel Carvalho
Veiga, Carla
Moura, Rute S.
Pinto, Jorge Correia
dc.subject.por.fl_str_mv Science & Technology
topic Science & Technology
description The glycoprotein 130 (gp130) dependent family of cytokines comprises interleukin-6 (IL-6), IL-11, leukemia inhibitory factor (LIF), cardiotrophin-like cytokine (CLC), ciliary neurotrophic factor (CNTF), cardiotrophin-1 (CT-1), and oncostatin M (OSM). These cytokines share the membrane gp130 as a common signal transducer. Recently, it was demonstrated that IL-6 promotes, whereas LIF inhibits fetal lung branching. Thus, in this study, the effects on fetal lung morphogenesis of the other classical members of the gp130-type cytokines (IL-11, CLC, CNTF, CT-1 and OSM) were investigated. We also provide the first description of these cytokines and their common gp130 receptor protein expression patterns during rat lung development. Fetal rat lung explants were cultured in vitro with increasing concentrations of IL-11, CLC, CNTF, CT-1 and OSM. Treated lung explants were morphometrically analyzed and assessed for MAPK, PI3K/AKT and STAT3 signaling modifications. IL-11, which similarly to IL-6 acts through a gp130 homodimer receptor, significantly stimulated lung growth via p38 phosphorylation. On the other hand, CLC, CNTF, CT-1 and OSM, whose receptors are gp130 heterodimers, inhibited lung growth acting in different signal-transducing pathways. Thus, the present study demonstrated that although cytokines of the gp130 family share a common signal transducer, there are specific biological activities for each cytokine on lung development. Indeed, cytokine signaling through gp130 homodimers stimulate, whereas cytokine signaling through gp130 heterodimers inhibit lung branching.
publishDate 2013
dc.date.none.fl_str_mv 2013-06
2013-06-01T00:00:00Z
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/24799
url http://hdl.handle.net/1822/24799
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1932-6203
10.1371/journal.pone.0067607
23826327
http://www.plosone.org
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dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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