Isoflavones as apoptosis inducers in human hepatoma HuH-7 cells.

Detalhes bibliográficos
Autor(a) principal: Mansoor, T.A.
Data de Publicação: 2011
Outros Autores: Ramalho, R.M., Luo, X., Ramalhete, Cátia, Rodrigues, C. M., Ferreira, Maria José
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10884/1316
Resumo: Nine flavonoids isolated from the ethyl acetate extract of Pycnanthus angolensis were assayed for their potential apoptosis induction activities in human hepatoma HuH-7 cells. These flavonoids include eight isoflavones, namely irilone (1), tectorigenine (2), formononetin (3), genistein (4), 2'-hydroxybiochanin A (5), mixture of biochanin A (6) and prunetin (7), and 4',7-dihydroxy-2'-methoxyisoflavan (8), and the flavanone liguiritigentin (9). Their chemical structures were characterized by spectroscopic methods including 2D NMR experiments. Methodology for cell death detection included the LDH assay, Hoechst staining, TUNEL staining and general caspase-3-like activity assay. The compounds tested showed higher apoptosis induction profiles in HuH-7 cells compared with the control. Caspase activity assays confirmed the apoptosis inducing activity of these flavonoids.
id RCAP_4975a87c198b2b535b1de5f30d5dcc32
oai_identifier_str oai:repositorio-cientifico.uatlantica.pt:10884/1316
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Isoflavones as apoptosis inducers in human hepatoma HuH-7 cells.Nine flavonoids isolated from the ethyl acetate extract of Pycnanthus angolensis were assayed for their potential apoptosis induction activities in human hepatoma HuH-7 cells. These flavonoids include eight isoflavones, namely irilone (1), tectorigenine (2), formononetin (3), genistein (4), 2'-hydroxybiochanin A (5), mixture of biochanin A (6) and prunetin (7), and 4',7-dihydroxy-2'-methoxyisoflavan (8), and the flavanone liguiritigentin (9). Their chemical structures were characterized by spectroscopic methods including 2D NMR experiments. Methodology for cell death detection included the LDH assay, Hoechst staining, TUNEL staining and general caspase-3-like activity assay. The compounds tested showed higher apoptosis induction profiles in HuH-7 cells compared with the control. Caspase activity assays confirmed the apoptosis inducing activity of these flavonoids.Phytotherapy Research2018-08-28T10:39:55Z2011-12-01T00:00:00Z2011-12info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10884/1316engMansoor, T.A.Ramalho, R.M.Luo, X.Ramalhete, CátiaRodrigues, C. M.Ferreira, Maria Joséinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-04T11:08:02Zoai:repositorio-cientifico.uatlantica.pt:10884/1316Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:29:53.584281Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Isoflavones as apoptosis inducers in human hepatoma HuH-7 cells.
title Isoflavones as apoptosis inducers in human hepatoma HuH-7 cells.
spellingShingle Isoflavones as apoptosis inducers in human hepatoma HuH-7 cells.
Mansoor, T.A.
title_short Isoflavones as apoptosis inducers in human hepatoma HuH-7 cells.
title_full Isoflavones as apoptosis inducers in human hepatoma HuH-7 cells.
title_fullStr Isoflavones as apoptosis inducers in human hepatoma HuH-7 cells.
title_full_unstemmed Isoflavones as apoptosis inducers in human hepatoma HuH-7 cells.
title_sort Isoflavones as apoptosis inducers in human hepatoma HuH-7 cells.
author Mansoor, T.A.
author_facet Mansoor, T.A.
Ramalho, R.M.
Luo, X.
Ramalhete, Cátia
Rodrigues, C. M.
Ferreira, Maria José
author_role author
author2 Ramalho, R.M.
Luo, X.
Ramalhete, Cátia
Rodrigues, C. M.
Ferreira, Maria José
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Mansoor, T.A.
Ramalho, R.M.
Luo, X.
Ramalhete, Cátia
Rodrigues, C. M.
Ferreira, Maria José
description Nine flavonoids isolated from the ethyl acetate extract of Pycnanthus angolensis were assayed for their potential apoptosis induction activities in human hepatoma HuH-7 cells. These flavonoids include eight isoflavones, namely irilone (1), tectorigenine (2), formononetin (3), genistein (4), 2'-hydroxybiochanin A (5), mixture of biochanin A (6) and prunetin (7), and 4',7-dihydroxy-2'-methoxyisoflavan (8), and the flavanone liguiritigentin (9). Their chemical structures were characterized by spectroscopic methods including 2D NMR experiments. Methodology for cell death detection included the LDH assay, Hoechst staining, TUNEL staining and general caspase-3-like activity assay. The compounds tested showed higher apoptosis induction profiles in HuH-7 cells compared with the control. Caspase activity assays confirmed the apoptosis inducing activity of these flavonoids.
publishDate 2011
dc.date.none.fl_str_mv 2011-12-01T00:00:00Z
2011-12
2018-08-28T10:39:55Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10884/1316
url http://hdl.handle.net/10884/1316
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Phytotherapy Research
publisher.none.fl_str_mv Phytotherapy Research
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799136781027770368

Registros relacionados