Label-free quantum dot conjugates for human protein IL-2 based on molecularly imprinted polymers
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.22/19485 |
Resumo: | Herein, the development of a fluorescent-based sensor by combining quantum dots (QDs) with molecularly-imprinted technology (MIT), intensively optimized to generate exceptional operating features is presented. This sensor is designed to target human interleukin-2 (IL-2) in synthetic human serum. IL-2 is a regulatory protein released as a triggered response from the immune system towards an inflammation. For this purpose, cadmium telluride (CdTe) QDs are prepared with 3-mercaptopropionic acid (MPA) and modified afterwards to produce an IL-2 imprinted polymer with methacrylic acid and N,N´-methylenebis(acrylamide), upon removal of the template under optimized conditions. During IL-2 rebinding, the fluorescence intensity of CdTe@MPA QDs is quenched in a concentration dependent manner. Using surface imprinting technology, the optimal fluorescence signals yielded a linear response versus logarithm of IL-2 concentration from 35 fg/ml to 39 pg/ml, in a 1000-fold diluted synthetic human serum. The limit of detection obtained is 5.91 fg/ml, lying below the concentration levels of IL-2 with clinical interest for cancer diagnosis (9.4–19.2 pg/ml). Overall, the method presented herein is a demonstration that the combination of MIP and QDs for protein detection constitutes a powerful tool in clinical analysis, providing low cost, sensitive and quick responses. The same concept may be further extended to other proteins of interest. |
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Label-free quantum dot conjugates for human protein IL-2 based on molecularly imprinted polymersQuantum dotsMolecularly imprinted polymerConjugated-QdsProteinInterleukin-2Cancer biomarkerHerein, the development of a fluorescent-based sensor by combining quantum dots (QDs) with molecularly-imprinted technology (MIT), intensively optimized to generate exceptional operating features is presented. This sensor is designed to target human interleukin-2 (IL-2) in synthetic human serum. IL-2 is a regulatory protein released as a triggered response from the immune system towards an inflammation. For this purpose, cadmium telluride (CdTe) QDs are prepared with 3-mercaptopropionic acid (MPA) and modified afterwards to produce an IL-2 imprinted polymer with methacrylic acid and N,N´-methylenebis(acrylamide), upon removal of the template under optimized conditions. During IL-2 rebinding, the fluorescence intensity of CdTe@MPA QDs is quenched in a concentration dependent manner. Using surface imprinting technology, the optimal fluorescence signals yielded a linear response versus logarithm of IL-2 concentration from 35 fg/ml to 39 pg/ml, in a 1000-fold diluted synthetic human serum. The limit of detection obtained is 5.91 fg/ml, lying below the concentration levels of IL-2 with clinical interest for cancer diagnosis (9.4–19.2 pg/ml). Overall, the method presented herein is a demonstration that the combination of MIP and QDs for protein detection constitutes a powerful tool in clinical analysis, providing low cost, sensitive and quick responses. The same concept may be further extended to other proteins of interest.The authors acknowledge the financial support of European Research Council though the Starting Grant, 3P’s Starting Grant/ERC (GA 311086)ElsevierRepositório Científico do Instituto Politécnico do PortoPiloto, Ana Margarida L.Ribeiro, David S.M.Rodrigues, S. Sofia M.Santos, João L.M.Ferreira Sales, Maria Goreti2022-01-14T11:31:55Z20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.22/19485engPiloto AML, Ribeiro DSM, Rodrigues SSM, Santos JLM, Ferreira Sales MG, Label-free quantum dot conjugates for human protein IL-2 based on molecularly imprinted polymers, Sensors and Actuators: B. Chemical (2019), doi: https://doi.org/10.1016/j.snb.2019.1273430925-400510.1016/j.snb.2019.127343info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-13T13:13:52Zoai:recipp.ipp.pt:10400.22/19485Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:39:26.644680Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Label-free quantum dot conjugates for human protein IL-2 based on molecularly imprinted polymers |
title |
Label-free quantum dot conjugates for human protein IL-2 based on molecularly imprinted polymers |
spellingShingle |
Label-free quantum dot conjugates for human protein IL-2 based on molecularly imprinted polymers Piloto, Ana Margarida L. Quantum dots Molecularly imprinted polymer Conjugated-Qds Protein Interleukin-2 Cancer biomarker |
title_short |
Label-free quantum dot conjugates for human protein IL-2 based on molecularly imprinted polymers |
title_full |
Label-free quantum dot conjugates for human protein IL-2 based on molecularly imprinted polymers |
title_fullStr |
Label-free quantum dot conjugates for human protein IL-2 based on molecularly imprinted polymers |
title_full_unstemmed |
Label-free quantum dot conjugates for human protein IL-2 based on molecularly imprinted polymers |
title_sort |
Label-free quantum dot conjugates for human protein IL-2 based on molecularly imprinted polymers |
author |
Piloto, Ana Margarida L. |
author_facet |
Piloto, Ana Margarida L. Ribeiro, David S.M. Rodrigues, S. Sofia M. Santos, João L.M. Ferreira Sales, Maria Goreti |
author_role |
author |
author2 |
Ribeiro, David S.M. Rodrigues, S. Sofia M. Santos, João L.M. Ferreira Sales, Maria Goreti |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Politécnico do Porto |
dc.contributor.author.fl_str_mv |
Piloto, Ana Margarida L. Ribeiro, David S.M. Rodrigues, S. Sofia M. Santos, João L.M. Ferreira Sales, Maria Goreti |
dc.subject.por.fl_str_mv |
Quantum dots Molecularly imprinted polymer Conjugated-Qds Protein Interleukin-2 Cancer biomarker |
topic |
Quantum dots Molecularly imprinted polymer Conjugated-Qds Protein Interleukin-2 Cancer biomarker |
description |
Herein, the development of a fluorescent-based sensor by combining quantum dots (QDs) with molecularly-imprinted technology (MIT), intensively optimized to generate exceptional operating features is presented. This sensor is designed to target human interleukin-2 (IL-2) in synthetic human serum. IL-2 is a regulatory protein released as a triggered response from the immune system towards an inflammation. For this purpose, cadmium telluride (CdTe) QDs are prepared with 3-mercaptopropionic acid (MPA) and modified afterwards to produce an IL-2 imprinted polymer with methacrylic acid and N,N´-methylenebis(acrylamide), upon removal of the template under optimized conditions. During IL-2 rebinding, the fluorescence intensity of CdTe@MPA QDs is quenched in a concentration dependent manner. Using surface imprinting technology, the optimal fluorescence signals yielded a linear response versus logarithm of IL-2 concentration from 35 fg/ml to 39 pg/ml, in a 1000-fold diluted synthetic human serum. The limit of detection obtained is 5.91 fg/ml, lying below the concentration levels of IL-2 with clinical interest for cancer diagnosis (9.4–19.2 pg/ml). Overall, the method presented herein is a demonstration that the combination of MIP and QDs for protein detection constitutes a powerful tool in clinical analysis, providing low cost, sensitive and quick responses. The same concept may be further extended to other proteins of interest. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020 2020-01-01T00:00:00Z 2022-01-14T11:31:55Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.22/19485 |
url |
http://hdl.handle.net/10400.22/19485 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Piloto AML, Ribeiro DSM, Rodrigues SSM, Santos JLM, Ferreira Sales MG, Label-free quantum dot conjugates for human protein IL-2 based on molecularly imprinted polymers, Sensors and Actuators: B. Chemical (2019), doi: https://doi.org/10.1016/j.snb.2019.127343 0925-4005 10.1016/j.snb.2019.127343 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799131483022032896 |