Exploring the Involvement of the Alternative Respiratory Pathway in Pisum sativum L. Seed Germination

Detalhes bibliográficos
Autor(a) principal: Rodrigues, Lénia
Data de Publicação: 2022
Outros Autores: Claudino, Ricardo, Groot, Steven P.C., Hohmann, Pierre, Nogales, Amaia, Hansen, Lee, Cardoso, Hélia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/24183
Resumo: Proceeding Paper
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spelling Exploring the Involvement of the Alternative Respiratory Pathway in Pisum sativum L. Seed GerminationLIVESEEDpeaAOXalternative respiratory pathwayseed germinationProceeding PaperOrganic agriculture, recognized as a more sustainable agricultural system, strongly de-pends on the use of highly resilient genotypes. Resilient seeds, with increased tolerance to germinate and provide vigorous seedlings under environmental stresses, currently represent one of the most important agronomical traits. Seed germination involves the activation of several metabolic path-ways, including cellular respiration. Alternative oxidase (AOX), a key enzyme in the alternative respiratory pathway, plays a crucial role in regulating cell reprogramming by controlling metabolic transitions related to the cellular redox state and the variable carbon balance. The involvement of the alternative respiratory pathway during germination was explored by analysis of PsAOX gene/protein expression. Seeds of four Pisum sativum L. cultivars (‘Respect-1′, ‘S134′, ‘G78′ and ‘S91′) were imbibed in sterile tap water for 16 h and metabolic parameters measured by calorespirometry (heat and CO2 emission rates) in a Multi-Cell Differential Scanning Calorimeter in isothermal mode at 25 °C. The involvement of PsAOX was evaluated by transcript quantification (PsAOX1, PsAOX2a, and PsAOX2b) through RT-qPCR, and by of analysing the PsAOX expression through Western blot. The results demonstrate that the cv. ‘S91′, characterized by a low germination rate, exhibited the lowest metabolic heat and CO2 emission rate. However, contrary to expectations, PsAOX transcript accumulation and PsAOX protein expression were significantly higher for ‘S91′ than for the other cultivars. These results indicate that higher levels of AOX (transcript and protein) could be linked to lower metabolic rates for embryo growth when seed germination is compromisedMDPIRepositório da Universidade de LisboaRodrigues, LéniaClaudino, RicardoGroot, Steven P.C.Hohmann, PierreNogales, AmaiaHansen, LeeCardoso, Hélia2022-04-28T08:52:47Z20222022-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/24183engRodrigues, L.; Claudino, R.; Groot, S.; Hohmann, P.; Nogales, A.; Hansen, L. Exploring the Involvement of the Alternative Respiratory Pathway in Pisum sativum L. Seed Germination. Biol. Life Sci. Forum 2022, 2, x.https://doi.org/10.3390/xxxxxinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:53:50Zoai:www.repository.utl.pt:10400.5/24183Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:08:17.454095Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Exploring the Involvement of the Alternative Respiratory Pathway in Pisum sativum L. Seed Germination
title Exploring the Involvement of the Alternative Respiratory Pathway in Pisum sativum L. Seed Germination
spellingShingle Exploring the Involvement of the Alternative Respiratory Pathway in Pisum sativum L. Seed Germination
Rodrigues, Lénia
LIVESEED
pea
AOX
alternative respiratory pathway
seed germination
title_short Exploring the Involvement of the Alternative Respiratory Pathway in Pisum sativum L. Seed Germination
title_full Exploring the Involvement of the Alternative Respiratory Pathway in Pisum sativum L. Seed Germination
title_fullStr Exploring the Involvement of the Alternative Respiratory Pathway in Pisum sativum L. Seed Germination
title_full_unstemmed Exploring the Involvement of the Alternative Respiratory Pathway in Pisum sativum L. Seed Germination
title_sort Exploring the Involvement of the Alternative Respiratory Pathway in Pisum sativum L. Seed Germination
author Rodrigues, Lénia
author_facet Rodrigues, Lénia
Claudino, Ricardo
Groot, Steven P.C.
Hohmann, Pierre
Nogales, Amaia
Hansen, Lee
Cardoso, Hélia
author_role author
author2 Claudino, Ricardo
Groot, Steven P.C.
Hohmann, Pierre
Nogales, Amaia
Hansen, Lee
Cardoso, Hélia
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Rodrigues, Lénia
Claudino, Ricardo
Groot, Steven P.C.
Hohmann, Pierre
Nogales, Amaia
Hansen, Lee
Cardoso, Hélia
dc.subject.por.fl_str_mv LIVESEED
pea
AOX
alternative respiratory pathway
seed germination
topic LIVESEED
pea
AOX
alternative respiratory pathway
seed germination
description Proceeding Paper
publishDate 2022
dc.date.none.fl_str_mv 2022-04-28T08:52:47Z
2022
2022-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/24183
url http://hdl.handle.net/10400.5/24183
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Rodrigues, L.; Claudino, R.; Groot, S.; Hohmann, P.; Nogales, A.; Hansen, L. Exploring the Involvement of the Alternative Respiratory Pathway in Pisum sativum L. Seed Germination. Biol. Life Sci. Forum 2022, 2, x.
https://doi.org/10.3390/xxxxx
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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