Exploring the Involvement of the Alternative Respiratory Pathway in Pisum sativum L. Seed Germination
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.5/24183 |
Resumo: | Proceeding Paper |
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7160 |
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Exploring the Involvement of the Alternative Respiratory Pathway in Pisum sativum L. Seed GerminationLIVESEEDpeaAOXalternative respiratory pathwayseed germinationProceeding PaperOrganic agriculture, recognized as a more sustainable agricultural system, strongly de-pends on the use of highly resilient genotypes. Resilient seeds, with increased tolerance to germinate and provide vigorous seedlings under environmental stresses, currently represent one of the most important agronomical traits. Seed germination involves the activation of several metabolic path-ways, including cellular respiration. Alternative oxidase (AOX), a key enzyme in the alternative respiratory pathway, plays a crucial role in regulating cell reprogramming by controlling metabolic transitions related to the cellular redox state and the variable carbon balance. The involvement of the alternative respiratory pathway during germination was explored by analysis of PsAOX gene/protein expression. Seeds of four Pisum sativum L. cultivars (‘Respect-1′, ‘S134′, ‘G78′ and ‘S91′) were imbibed in sterile tap water for 16 h and metabolic parameters measured by calorespirometry (heat and CO2 emission rates) in a Multi-Cell Differential Scanning Calorimeter in isothermal mode at 25 °C. The involvement of PsAOX was evaluated by transcript quantification (PsAOX1, PsAOX2a, and PsAOX2b) through RT-qPCR, and by of analysing the PsAOX expression through Western blot. The results demonstrate that the cv. ‘S91′, characterized by a low germination rate, exhibited the lowest metabolic heat and CO2 emission rate. However, contrary to expectations, PsAOX transcript accumulation and PsAOX protein expression were significantly higher for ‘S91′ than for the other cultivars. These results indicate that higher levels of AOX (transcript and protein) could be linked to lower metabolic rates for embryo growth when seed germination is compromisedMDPIRepositório da Universidade de LisboaRodrigues, LéniaClaudino, RicardoGroot, Steven P.C.Hohmann, PierreNogales, AmaiaHansen, LeeCardoso, Hélia2022-04-28T08:52:47Z20222022-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/24183engRodrigues, L.; Claudino, R.; Groot, S.; Hohmann, P.; Nogales, A.; Hansen, L. Exploring the Involvement of the Alternative Respiratory Pathway in Pisum sativum L. Seed Germination. Biol. Life Sci. Forum 2022, 2, x.https://doi.org/10.3390/xxxxxinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:53:50Zoai:www.repository.utl.pt:10400.5/24183Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:08:17.454095Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Exploring the Involvement of the Alternative Respiratory Pathway in Pisum sativum L. Seed Germination |
title |
Exploring the Involvement of the Alternative Respiratory Pathway in Pisum sativum L. Seed Germination |
spellingShingle |
Exploring the Involvement of the Alternative Respiratory Pathway in Pisum sativum L. Seed Germination Rodrigues, Lénia LIVESEED pea AOX alternative respiratory pathway seed germination |
title_short |
Exploring the Involvement of the Alternative Respiratory Pathway in Pisum sativum L. Seed Germination |
title_full |
Exploring the Involvement of the Alternative Respiratory Pathway in Pisum sativum L. Seed Germination |
title_fullStr |
Exploring the Involvement of the Alternative Respiratory Pathway in Pisum sativum L. Seed Germination |
title_full_unstemmed |
Exploring the Involvement of the Alternative Respiratory Pathway in Pisum sativum L. Seed Germination |
title_sort |
Exploring the Involvement of the Alternative Respiratory Pathway in Pisum sativum L. Seed Germination |
author |
Rodrigues, Lénia |
author_facet |
Rodrigues, Lénia Claudino, Ricardo Groot, Steven P.C. Hohmann, Pierre Nogales, Amaia Hansen, Lee Cardoso, Hélia |
author_role |
author |
author2 |
Claudino, Ricardo Groot, Steven P.C. Hohmann, Pierre Nogales, Amaia Hansen, Lee Cardoso, Hélia |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
dc.contributor.author.fl_str_mv |
Rodrigues, Lénia Claudino, Ricardo Groot, Steven P.C. Hohmann, Pierre Nogales, Amaia Hansen, Lee Cardoso, Hélia |
dc.subject.por.fl_str_mv |
LIVESEED pea AOX alternative respiratory pathway seed germination |
topic |
LIVESEED pea AOX alternative respiratory pathway seed germination |
description |
Proceeding Paper |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-04-28T08:52:47Z 2022 2022-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.5/24183 |
url |
http://hdl.handle.net/10400.5/24183 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Rodrigues, L.; Claudino, R.; Groot, S.; Hohmann, P.; Nogales, A.; Hansen, L. Exploring the Involvement of the Alternative Respiratory Pathway in Pisum sativum L. Seed Germination. Biol. Life Sci. Forum 2022, 2, x. https://doi.org/10.3390/xxxxx |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
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1799131176966815744 |