Smoothing membrane protein structure determination by initial upstream stage improvements

Detalhes bibliográficos
Autor(a) principal: Pedro, Augusto Quaresma
Data de Publicação: 2019
Outros Autores: Queiroz, João António, Passarinha, Luís António
Tipo de documento: Outros
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/100997
Resumo: Pest-C/SAU/UI709/2011 Project POCI-01-0145-FEDER-007491 UID/CTM/50011/2019 UID/Multi/04378/2019 POCI-01-0145-FEDER-030840
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repository_id_str 7160
spelling Smoothing membrane protein structure determination by initial upstream stage improvementsCodon usageHostMembrane proteinOptimizationProductionProtein 3D-structureQuality controlStructure determinationBiotechnologyApplied Microbiology and BiotechnologyPest-C/SAU/UI709/2011 Project POCI-01-0145-FEDER-007491 UID/CTM/50011/2019 UID/Multi/04378/2019 POCI-01-0145-FEDER-030840Membrane proteins (MP) constitute 20–30% of all proteins encoded by the genome of various organisms and perform a wide range of essential biological functions. However, despite they represent the largest class of protein drug targets, a relatively small number high-resolution 3D structures have been obtained yet. Membrane protein biogenesis is more complex than that of the soluble proteins and its recombinant biosynthesis has been a major drawback, thus delaying their further structural characterization. Indeed, the major limitation in structure determination of MP is the low yield achieved in recombinant expression, usually coupled to low functionality, pinpointing the optimization target in recombinant MP research. Recently, the growing attention that have been dedicated to the upstream stage of MP bioprocesses allowed great advances, permitting the evolution of the number of MP solved structures. In this review, we analyse and discuss effective solutions and technical advances at the level of the upstream stage using prokaryotic and eukaryotic organisms foreseeing an increase in expression yields of correctly folded MP and that may facilitate the determination of their three-dimensional structure. A section on techniques used to protein quality control and further structure determination of MP is also included. Lastly, a critical assessment of major factors contributing for a good decision-making process related to the upstream stage of MP is presented.UCIBIO - Applied Molecular Biosciences UnitDQ - Departamento de QuímicaRUNPedro, Augusto QuaresmaQueiroz, João AntónioPassarinha, Luís António2020-07-16T22:49:50Z2019-07-202019-07-20T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/other18application/pdfhttp://hdl.handle.net/10362/100997eng0175-7598PURE: 18784671https://doi.org/10.1007/s00253-019-09873-1info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-22T17:46:32Zoai:run.unl.pt:10362/100997Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-22T17:46:32Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Smoothing membrane protein structure determination by initial upstream stage improvements
title Smoothing membrane protein structure determination by initial upstream stage improvements
spellingShingle Smoothing membrane protein structure determination by initial upstream stage improvements
Pedro, Augusto Quaresma
Codon usage
Host
Membrane protein
Optimization
Production
Protein 3D-structure
Quality control
Structure determination
Biotechnology
Applied Microbiology and Biotechnology
title_short Smoothing membrane protein structure determination by initial upstream stage improvements
title_full Smoothing membrane protein structure determination by initial upstream stage improvements
title_fullStr Smoothing membrane protein structure determination by initial upstream stage improvements
title_full_unstemmed Smoothing membrane protein structure determination by initial upstream stage improvements
title_sort Smoothing membrane protein structure determination by initial upstream stage improvements
author Pedro, Augusto Quaresma
author_facet Pedro, Augusto Quaresma
Queiroz, João António
Passarinha, Luís António
author_role author
author2 Queiroz, João António
Passarinha, Luís António
author2_role author
author
dc.contributor.none.fl_str_mv UCIBIO - Applied Molecular Biosciences Unit
DQ - Departamento de Química
RUN
dc.contributor.author.fl_str_mv Pedro, Augusto Quaresma
Queiroz, João António
Passarinha, Luís António
dc.subject.por.fl_str_mv Codon usage
Host
Membrane protein
Optimization
Production
Protein 3D-structure
Quality control
Structure determination
Biotechnology
Applied Microbiology and Biotechnology
topic Codon usage
Host
Membrane protein
Optimization
Production
Protein 3D-structure
Quality control
Structure determination
Biotechnology
Applied Microbiology and Biotechnology
description Pest-C/SAU/UI709/2011 Project POCI-01-0145-FEDER-007491 UID/CTM/50011/2019 UID/Multi/04378/2019 POCI-01-0145-FEDER-030840
publishDate 2019
dc.date.none.fl_str_mv 2019-07-20
2019-07-20T00:00:00Z
2020-07-16T22:49:50Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/other
format other
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/100997
url http://hdl.handle.net/10362/100997
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0175-7598
PURE: 18784671
https://doi.org/10.1007/s00253-019-09873-1
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 18
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
_version_ 1817545749755330560

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