The comet assay in animal models: From bugs to whales – (Part 2 Vertebrates)
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.18/6605 |
Resumo: | The comet assay has become one of the methods of choice for the evaluation and measurement of DNA damage. It is sensitive, quick to perform and relatively affordable for the evaluation of DNA damage and repair at the level of individual cells. The comet assay can be applied to virtually any cell type derived from different organs and tissues. Even though the comet assay is predominantly used on human cells, the application of the assay for the evaluation of DNA damage in yeast, plant and animal cells is also quite high, especially in terms of biomonitoring. The present extensive overview on the usage of the comet assay in animal models will cover both terrestrial and water environments. The first part of the review was focused on studies describing the comet assay applied in invertebrates. The second part of the review, (Part 2) will discuss the application of the comet assay in vertebrates covering cyclostomata, fishes, amphibians, reptiles, birds and mammals, in addition to chordates that are regarded as a transitional form towards vertebrates. Besides numerous vertebrate species, the assay is also performed on a range of cells, which includes blood, liver, kidney, brain, gill, bone marrow and sperm cells. These cells are readily used for the evaluation of a wide spectrum of genotoxic agents both in vitro and in vivo. Moreover, the use of vertebrate models and their role in environmental biomonitoring will also be discussed as well as the comparison of the use of the comet assay in vertebrate and human models in line with ethical principles. Although the comet assay in vertebrates is most commonly used in laboratory animals such as mice, rats and lately zebrafish, this paper will only briefly review its use regarding laboratory animal models and rather give special emphasis to the increasing usage of the assay in domestic and wildlife animals as well as in various ecotoxicological studies. |
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The comet assay in animal models: From bugs to whales – (Part 2 Vertebrates)AnimalsComet AssayDNA DamageEnvironmental MonitoringHumansModels, AnimalVertebratesWhalesGenotoxicidade AmbientalThe comet assay has become one of the methods of choice for the evaluation and measurement of DNA damage. It is sensitive, quick to perform and relatively affordable for the evaluation of DNA damage and repair at the level of individual cells. The comet assay can be applied to virtually any cell type derived from different organs and tissues. Even though the comet assay is predominantly used on human cells, the application of the assay for the evaluation of DNA damage in yeast, plant and animal cells is also quite high, especially in terms of biomonitoring. The present extensive overview on the usage of the comet assay in animal models will cover both terrestrial and water environments. The first part of the review was focused on studies describing the comet assay applied in invertebrates. The second part of the review, (Part 2) will discuss the application of the comet assay in vertebrates covering cyclostomata, fishes, amphibians, reptiles, birds and mammals, in addition to chordates that are regarded as a transitional form towards vertebrates. Besides numerous vertebrate species, the assay is also performed on a range of cells, which includes blood, liver, kidney, brain, gill, bone marrow and sperm cells. These cells are readily used for the evaluation of a wide spectrum of genotoxic agents both in vitro and in vivo. Moreover, the use of vertebrate models and their role in environmental biomonitoring will also be discussed as well as the comparison of the use of the comet assay in vertebrate and human models in line with ethical principles. Although the comet assay in vertebrates is most commonly used in laboratory animals such as mice, rats and lately zebrafish, this paper will only briefly review its use regarding laboratory animal models and rather give special emphasis to the increasing usage of the assay in domestic and wildlife animals as well as in various ecotoxicological studies.This work was supported by the European Cooperation in Science and Technology (CA COST Action CA15132 – The comet assay as a human biomonitoring tool (hCOMET)).ElsevierRepositório Científico do Instituto Nacional de SaúdeGajski, GoranŽegura, BojanaLadeira, CarinaNovak, MatjažSramkova, MonikaPourrut, BertrandDel Bo’, CristianMilić, MirtaGutzkow, Kristine BjerveCosta, SolangeDusinska, MariaBrunborg, GunnarCollins, Andrew2020-05-06T16:17:40Z2019-04-202019-04-20T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/6605engMutat Res. Jul-Sep 2019;781:130-164. doi: 10.1016/j.mrrev.2019.04.002. Epub 2019 Apr 20. Review1383-574210.1016/j.mrrev.2019.04.002info:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:41:45Zoai:repositorio.insa.pt:10400.18/6605Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:41:41.118176Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
The comet assay in animal models: From bugs to whales – (Part 2 Vertebrates) |
title |
The comet assay in animal models: From bugs to whales – (Part 2 Vertebrates) |
spellingShingle |
The comet assay in animal models: From bugs to whales – (Part 2 Vertebrates) Gajski, Goran Animals Comet Assay DNA Damage Environmental Monitoring Humans Models, Animal Vertebrates Whales Genotoxicidade Ambiental |
title_short |
The comet assay in animal models: From bugs to whales – (Part 2 Vertebrates) |
title_full |
The comet assay in animal models: From bugs to whales – (Part 2 Vertebrates) |
title_fullStr |
The comet assay in animal models: From bugs to whales – (Part 2 Vertebrates) |
title_full_unstemmed |
The comet assay in animal models: From bugs to whales – (Part 2 Vertebrates) |
title_sort |
The comet assay in animal models: From bugs to whales – (Part 2 Vertebrates) |
author |
Gajski, Goran |
author_facet |
Gajski, Goran Žegura, Bojana Ladeira, Carina Novak, Matjaž Sramkova, Monika Pourrut, Bertrand Del Bo’, Cristian Milić, Mirta Gutzkow, Kristine Bjerve Costa, Solange Dusinska, Maria Brunborg, Gunnar Collins, Andrew |
author_role |
author |
author2 |
Žegura, Bojana Ladeira, Carina Novak, Matjaž Sramkova, Monika Pourrut, Bertrand Del Bo’, Cristian Milić, Mirta Gutzkow, Kristine Bjerve Costa, Solange Dusinska, Maria Brunborg, Gunnar Collins, Andrew |
author2_role |
author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Nacional de Saúde |
dc.contributor.author.fl_str_mv |
Gajski, Goran Žegura, Bojana Ladeira, Carina Novak, Matjaž Sramkova, Monika Pourrut, Bertrand Del Bo’, Cristian Milić, Mirta Gutzkow, Kristine Bjerve Costa, Solange Dusinska, Maria Brunborg, Gunnar Collins, Andrew |
dc.subject.por.fl_str_mv |
Animals Comet Assay DNA Damage Environmental Monitoring Humans Models, Animal Vertebrates Whales Genotoxicidade Ambiental |
topic |
Animals Comet Assay DNA Damage Environmental Monitoring Humans Models, Animal Vertebrates Whales Genotoxicidade Ambiental |
description |
The comet assay has become one of the methods of choice for the evaluation and measurement of DNA damage. It is sensitive, quick to perform and relatively affordable for the evaluation of DNA damage and repair at the level of individual cells. The comet assay can be applied to virtually any cell type derived from different organs and tissues. Even though the comet assay is predominantly used on human cells, the application of the assay for the evaluation of DNA damage in yeast, plant and animal cells is also quite high, especially in terms of biomonitoring. The present extensive overview on the usage of the comet assay in animal models will cover both terrestrial and water environments. The first part of the review was focused on studies describing the comet assay applied in invertebrates. The second part of the review, (Part 2) will discuss the application of the comet assay in vertebrates covering cyclostomata, fishes, amphibians, reptiles, birds and mammals, in addition to chordates that are regarded as a transitional form towards vertebrates. Besides numerous vertebrate species, the assay is also performed on a range of cells, which includes blood, liver, kidney, brain, gill, bone marrow and sperm cells. These cells are readily used for the evaluation of a wide spectrum of genotoxic agents both in vitro and in vivo. Moreover, the use of vertebrate models and their role in environmental biomonitoring will also be discussed as well as the comparison of the use of the comet assay in vertebrate and human models in line with ethical principles. Although the comet assay in vertebrates is most commonly used in laboratory animals such as mice, rats and lately zebrafish, this paper will only briefly review its use regarding laboratory animal models and rather give special emphasis to the increasing usage of the assay in domestic and wildlife animals as well as in various ecotoxicological studies. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-04-20 2019-04-20T00:00:00Z 2020-05-06T16:17:40Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.18/6605 |
url |
http://hdl.handle.net/10400.18/6605 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Mutat Res. Jul-Sep 2019;781:130-164. doi: 10.1016/j.mrrev.2019.04.002. Epub 2019 Apr 20. Review 1383-5742 10.1016/j.mrrev.2019.04.002 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/embargoedAccess |
eu_rights_str_mv |
embargoedAccess |
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application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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