The comet assay in animal models: From bugs to whales – (Part 2 Vertebrates)

Detalhes bibliográficos
Autor(a) principal: Gajski, Goran
Data de Publicação: 2019
Outros Autores: Žegura, Bojana, Ladeira, Carina, Novak, Matjaž, Sramkova, Monika, Pourrut, Bertrand, Del Bo’, Cristian, Milić, Mirta, Gutzkow, Kristine Bjerve, Costa, Solange, Dusinska, Maria, Brunborg, Gunnar, Collins, Andrew
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.18/6605
Resumo: The comet assay has become one of the methods of choice for the evaluation and measurement of DNA damage. It is sensitive, quick to perform and relatively affordable for the evaluation of DNA damage and repair at the level of individual cells. The comet assay can be applied to virtually any cell type derived from different organs and tissues. Even though the comet assay is predominantly used on human cells, the application of the assay for the evaluation of DNA damage in yeast, plant and animal cells is also quite high, especially in terms of biomonitoring. The present extensive overview on the usage of the comet assay in animal models will cover both terrestrial and water environments. The first part of the review was focused on studies describing the comet assay applied in invertebrates. The second part of the review, (Part 2) will discuss the application of the comet assay in vertebrates covering cyclostomata, fishes, amphibians, reptiles, birds and mammals, in addition to chordates that are regarded as a transitional form towards vertebrates. Besides numerous vertebrate species, the assay is also performed on a range of cells, which includes blood, liver, kidney, brain, gill, bone marrow and sperm cells. These cells are readily used for the evaluation of a wide spectrum of genotoxic agents both in vitro and in vivo. Moreover, the use of vertebrate models and their role in environmental biomonitoring will also be discussed as well as the comparison of the use of the comet assay in vertebrate and human models in line with ethical principles. Although the comet assay in vertebrates is most commonly used in laboratory animals such as mice, rats and lately zebrafish, this paper will only briefly review its use regarding laboratory animal models and rather give special emphasis to the increasing usage of the assay in domestic and wildlife animals as well as in various ecotoxicological studies.
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spelling The comet assay in animal models: From bugs to whales – (Part 2 Vertebrates)AnimalsComet AssayDNA DamageEnvironmental MonitoringHumansModels, AnimalVertebratesWhalesGenotoxicidade AmbientalThe comet assay has become one of the methods of choice for the evaluation and measurement of DNA damage. It is sensitive, quick to perform and relatively affordable for the evaluation of DNA damage and repair at the level of individual cells. The comet assay can be applied to virtually any cell type derived from different organs and tissues. Even though the comet assay is predominantly used on human cells, the application of the assay for the evaluation of DNA damage in yeast, plant and animal cells is also quite high, especially in terms of biomonitoring. The present extensive overview on the usage of the comet assay in animal models will cover both terrestrial and water environments. The first part of the review was focused on studies describing the comet assay applied in invertebrates. The second part of the review, (Part 2) will discuss the application of the comet assay in vertebrates covering cyclostomata, fishes, amphibians, reptiles, birds and mammals, in addition to chordates that are regarded as a transitional form towards vertebrates. Besides numerous vertebrate species, the assay is also performed on a range of cells, which includes blood, liver, kidney, brain, gill, bone marrow and sperm cells. These cells are readily used for the evaluation of a wide spectrum of genotoxic agents both in vitro and in vivo. Moreover, the use of vertebrate models and their role in environmental biomonitoring will also be discussed as well as the comparison of the use of the comet assay in vertebrate and human models in line with ethical principles. Although the comet assay in vertebrates is most commonly used in laboratory animals such as mice, rats and lately zebrafish, this paper will only briefly review its use regarding laboratory animal models and rather give special emphasis to the increasing usage of the assay in domestic and wildlife animals as well as in various ecotoxicological studies.This work was supported by the European Cooperation in Science and Technology (CA COST Action CA15132 – The comet assay as a human biomonitoring tool (hCOMET)).ElsevierRepositório Científico do Instituto Nacional de SaúdeGajski, GoranŽegura, BojanaLadeira, CarinaNovak, MatjažSramkova, MonikaPourrut, BertrandDel Bo’, CristianMilić, MirtaGutzkow, Kristine BjerveCosta, SolangeDusinska, MariaBrunborg, GunnarCollins, Andrew2020-05-06T16:17:40Z2019-04-202019-04-20T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/6605engMutat Res. Jul-Sep 2019;781:130-164. doi: 10.1016/j.mrrev.2019.04.002. Epub 2019 Apr 20. Review1383-574210.1016/j.mrrev.2019.04.002info:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:41:45Zoai:repositorio.insa.pt:10400.18/6605Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:41:41.118176Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv The comet assay in animal models: From bugs to whales – (Part 2 Vertebrates)
title The comet assay in animal models: From bugs to whales – (Part 2 Vertebrates)
spellingShingle The comet assay in animal models: From bugs to whales – (Part 2 Vertebrates)
Gajski, Goran
Animals
Comet Assay
DNA Damage
Environmental Monitoring
Humans
Models, Animal
Vertebrates
Whales
Genotoxicidade Ambiental
title_short The comet assay in animal models: From bugs to whales – (Part 2 Vertebrates)
title_full The comet assay in animal models: From bugs to whales – (Part 2 Vertebrates)
title_fullStr The comet assay in animal models: From bugs to whales – (Part 2 Vertebrates)
title_full_unstemmed The comet assay in animal models: From bugs to whales – (Part 2 Vertebrates)
title_sort The comet assay in animal models: From bugs to whales – (Part 2 Vertebrates)
author Gajski, Goran
author_facet Gajski, Goran
Žegura, Bojana
Ladeira, Carina
Novak, Matjaž
Sramkova, Monika
Pourrut, Bertrand
Del Bo’, Cristian
Milić, Mirta
Gutzkow, Kristine Bjerve
Costa, Solange
Dusinska, Maria
Brunborg, Gunnar
Collins, Andrew
author_role author
author2 Žegura, Bojana
Ladeira, Carina
Novak, Matjaž
Sramkova, Monika
Pourrut, Bertrand
Del Bo’, Cristian
Milić, Mirta
Gutzkow, Kristine Bjerve
Costa, Solange
Dusinska, Maria
Brunborg, Gunnar
Collins, Andrew
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Nacional de Saúde
dc.contributor.author.fl_str_mv Gajski, Goran
Žegura, Bojana
Ladeira, Carina
Novak, Matjaž
Sramkova, Monika
Pourrut, Bertrand
Del Bo’, Cristian
Milić, Mirta
Gutzkow, Kristine Bjerve
Costa, Solange
Dusinska, Maria
Brunborg, Gunnar
Collins, Andrew
dc.subject.por.fl_str_mv Animals
Comet Assay
DNA Damage
Environmental Monitoring
Humans
Models, Animal
Vertebrates
Whales
Genotoxicidade Ambiental
topic Animals
Comet Assay
DNA Damage
Environmental Monitoring
Humans
Models, Animal
Vertebrates
Whales
Genotoxicidade Ambiental
description The comet assay has become one of the methods of choice for the evaluation and measurement of DNA damage. It is sensitive, quick to perform and relatively affordable for the evaluation of DNA damage and repair at the level of individual cells. The comet assay can be applied to virtually any cell type derived from different organs and tissues. Even though the comet assay is predominantly used on human cells, the application of the assay for the evaluation of DNA damage in yeast, plant and animal cells is also quite high, especially in terms of biomonitoring. The present extensive overview on the usage of the comet assay in animal models will cover both terrestrial and water environments. The first part of the review was focused on studies describing the comet assay applied in invertebrates. The second part of the review, (Part 2) will discuss the application of the comet assay in vertebrates covering cyclostomata, fishes, amphibians, reptiles, birds and mammals, in addition to chordates that are regarded as a transitional form towards vertebrates. Besides numerous vertebrate species, the assay is also performed on a range of cells, which includes blood, liver, kidney, brain, gill, bone marrow and sperm cells. These cells are readily used for the evaluation of a wide spectrum of genotoxic agents both in vitro and in vivo. Moreover, the use of vertebrate models and their role in environmental biomonitoring will also be discussed as well as the comparison of the use of the comet assay in vertebrate and human models in line with ethical principles. Although the comet assay in vertebrates is most commonly used in laboratory animals such as mice, rats and lately zebrafish, this paper will only briefly review its use regarding laboratory animal models and rather give special emphasis to the increasing usage of the assay in domestic and wildlife animals as well as in various ecotoxicological studies.
publishDate 2019
dc.date.none.fl_str_mv 2019-04-20
2019-04-20T00:00:00Z
2020-05-06T16:17:40Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.18/6605
url http://hdl.handle.net/10400.18/6605
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Mutat Res. Jul-Sep 2019;781:130-164. doi: 10.1016/j.mrrev.2019.04.002. Epub 2019 Apr 20. Review
1383-5742
10.1016/j.mrrev.2019.04.002
dc.rights.driver.fl_str_mv info:eu-repo/semantics/embargoedAccess
eu_rights_str_mv embargoedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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