Cilia motility studies in zebrafish embryos

Detalhes bibliográficos
Autor(a) principal: Ferreira, Rita Joana Rodrigues da Silva Rua
Data de Publicação: 2012
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/7984
Resumo: A thesis submitted in fulfilment of the requirements for the degree of Masters in Molecular Genetics and Biomedicine
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spelling Cilia motility studies in zebrafish embryosKupffer’s vesicleMotile ciliaCilia beat frequencyFluid-flowDeltaD-/- mutantsA thesis submitted in fulfilment of the requirements for the degree of Masters in Molecular Genetics and BiomedicineMotile ciliary dysfunctions cause specific Ciliopathies that affect mainly the respiratory tract, fertilization and left-right body establishment. The embryonic organ where left-right decisions are first taken is called the organizer, a ciliated organ where a leftward cilia driven fluid-flow is generated. The organizer is named node in the mouse and Kupffer’s vesicle (KV) in zebrafish. The correct left-right axis formation is highly dependent on signaling pathways downstream of such directional fluid-flow. Motile cilia need to be coordinated and Ciliary Beat Frequency (CBF) is characteristic of different types of cilia depending on their function. Using zebrafish as a model, our group has been studying cilia length regulation and motility in wild-type and deltaD-/- mutant embryos. Recently, we showed that Notch signalling was directly involved in the control of cilia length in the KV cells given that the deltaD-/- mutant present shorter KV cilia. The goal of this project was to characterize the CBF of deltaD-/- KV cilia vs. wild-type cilia and reveal how potential differences in CBF impact on KV fluid flow, using spectral analysis associated with highspeed videomicroscopy. By decomposing and comparing the obtained CBF with Fast Fourier Transform, we identified two major populations of motile cilia in wild-type as well as in deltaD-/- mutant embryos. However, we found the CBF populations had differential relative contributions and different distributions between wild-type and mutant embryos. Furthermore, by measuring the velocity of native particles we studied the KV fluid-flow and concluded that the dispersion of the flow velocity was much wider in the deltaD-/- mutants. On the other hand, based on a gene expression study of motility genes downstream of DeltaD, we concluded that motility related genes (dnah7, rsph3 and foxj1a) were deregulated in the mutants. During this project we generated data that led to new hypotheses that will allow us to test the causality between the described correlations.Faculdade de Ciências e TecnologiaLopes, SusanaGonçalves, PaulaRUNFerreira, Rita Joana Rodrigues da Silva Rua2012-10-16T11:29:40Z20122012-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10362/7984enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T03:40:20Zoai:run.unl.pt:10362/7984Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:17:52.009951Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Cilia motility studies in zebrafish embryos
title Cilia motility studies in zebrafish embryos
spellingShingle Cilia motility studies in zebrafish embryos
Ferreira, Rita Joana Rodrigues da Silva Rua
Kupffer’s vesicle
Motile cilia
Cilia beat frequency
Fluid-flow
DeltaD-/- mutants
title_short Cilia motility studies in zebrafish embryos
title_full Cilia motility studies in zebrafish embryos
title_fullStr Cilia motility studies in zebrafish embryos
title_full_unstemmed Cilia motility studies in zebrafish embryos
title_sort Cilia motility studies in zebrafish embryos
author Ferreira, Rita Joana Rodrigues da Silva Rua
author_facet Ferreira, Rita Joana Rodrigues da Silva Rua
author_role author
dc.contributor.none.fl_str_mv Lopes, Susana
Gonçalves, Paula
RUN
dc.contributor.author.fl_str_mv Ferreira, Rita Joana Rodrigues da Silva Rua
dc.subject.por.fl_str_mv Kupffer’s vesicle
Motile cilia
Cilia beat frequency
Fluid-flow
DeltaD-/- mutants
topic Kupffer’s vesicle
Motile cilia
Cilia beat frequency
Fluid-flow
DeltaD-/- mutants
description A thesis submitted in fulfilment of the requirements for the degree of Masters in Molecular Genetics and Biomedicine
publishDate 2012
dc.date.none.fl_str_mv 2012-10-16T11:29:40Z
2012
2012-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/7984
url http://hdl.handle.net/10362/7984
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Faculdade de Ciências e Tecnologia
publisher.none.fl_str_mv Faculdade de Ciências e Tecnologia
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799137825577238528

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