Dynamics of cilia length in left–right development

Detalhes bibliográficos
Autor(a) principal: Pintado, P.
Data de Publicação: 2017
Outros Autores: Sampaio, P., Tavares, B., Montenegro-Johnson, T.D., Smith, D.J., Lopes, S.S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://doi.org/10.1098/rsos.161102
Resumo: This work was supported by the Fundacao para a Ciencia e a Tecnologia (FCT-ANR/BEX-BID/0153/2012 research grant). T.D.M.-J. was supported by a Royal Commission for the Exhibition of 1851 Fellowship. S.S.L. was funded by FCT Investigator IF/00951/2012. B.T. was funded by FCT fellowship SFRH/BPD/77258/2011 and P.P. and P.S. were funded by the FCT-ANR/BEX-BID/0153/2012 research grant.
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spelling Dynamics of cilia length in left–right developmentCilia lengthFlow dynamicsLeft–right developmentThis work was supported by the Fundacao para a Ciencia e a Tecnologia (FCT-ANR/BEX-BID/0153/2012 research grant). T.D.M.-J. was supported by a Royal Commission for the Exhibition of 1851 Fellowship. S.S.L. was funded by FCT Investigator IF/00951/2012. B.T. was funded by FCT fellowship SFRH/BPD/77258/2011 and P.P. and P.S. were funded by the FCT-ANR/BEX-BID/0153/2012 research grant.Reduction in the length of motile cilia in the zebrafish left– right organizer (LRO), also known as Kupffer’s vesicle, has a large impact on left–right development. Here we demonstrate through genetic overexpression in zebrafish embryos and mathematical modelling that the impact of increased motile cilia length in embryonic LRO fluid flow is milder than that of short cilia. Through Arl13b overexpression, which increases cilia length without impacting cilia beat frequency, we show that the increase in cilium length is associated with a decrease in beat amplitude, resulting in similar flow strengths for Arl13b overexpression and wild-type (WT) embryos, which were not predicted by current theory. Longer cilia exhibit pronounced helical beat patterns and, consequently, lower beat amplitudes relative to WT, a result of an elastohydrodynamic shape transition. For long helical cilia, fluid dynamics modelling predicts a mild (approx. 12%) reduction in the torque exerted on the fluid relative to the WT, resulting in a proportional reduction in flow generation. This mild reduction is corroborated by experiments, providing a mechanism for the mild impact on organ situs. ©2017 The Authors.Centro de Estudos de Doenças Crónicas (CEDOC)NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)RUNPintado, P.Sampaio, P.Tavares, B.Montenegro-Johnson, T.D.Smith, D.J.Lopes, S.S.2017-10-26T22:03:11Z2017-032017-03-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://doi.org/10.1098/rsos.161102eng2054-5703PURE: 3226834https://www.scopus.com/inward/record.uri?eid=2-s2.0-85014758292&doi=10.1098%2frsos.161102&partnerID=40&md5=1eb0918e932ad5c3ec941bab9ab3c187https://doi.org/10.1098/rsos.161102info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:12:51Zoai:run.unl.pt:10362/24649Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:28:05.872347Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Dynamics of cilia length in left–right development
title Dynamics of cilia length in left–right development
spellingShingle Dynamics of cilia length in left–right development
Pintado, P.
Cilia length
Flow dynamics
Left–right development
title_short Dynamics of cilia length in left–right development
title_full Dynamics of cilia length in left–right development
title_fullStr Dynamics of cilia length in left–right development
title_full_unstemmed Dynamics of cilia length in left–right development
title_sort Dynamics of cilia length in left–right development
author Pintado, P.
author_facet Pintado, P.
Sampaio, P.
Tavares, B.
Montenegro-Johnson, T.D.
Smith, D.J.
Lopes, S.S.
author_role author
author2 Sampaio, P.
Tavares, B.
Montenegro-Johnson, T.D.
Smith, D.J.
Lopes, S.S.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Centro de Estudos de Doenças Crónicas (CEDOC)
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
RUN
dc.contributor.author.fl_str_mv Pintado, P.
Sampaio, P.
Tavares, B.
Montenegro-Johnson, T.D.
Smith, D.J.
Lopes, S.S.
dc.subject.por.fl_str_mv Cilia length
Flow dynamics
Left–right development
topic Cilia length
Flow dynamics
Left–right development
description This work was supported by the Fundacao para a Ciencia e a Tecnologia (FCT-ANR/BEX-BID/0153/2012 research grant). T.D.M.-J. was supported by a Royal Commission for the Exhibition of 1851 Fellowship. S.S.L. was funded by FCT Investigator IF/00951/2012. B.T. was funded by FCT fellowship SFRH/BPD/77258/2011 and P.P. and P.S. were funded by the FCT-ANR/BEX-BID/0153/2012 research grant.
publishDate 2017
dc.date.none.fl_str_mv 2017-10-26T22:03:11Z
2017-03
2017-03-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://doi.org/10.1098/rsos.161102
url https://doi.org/10.1098/rsos.161102
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2054-5703
PURE: 3226834
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85014758292&doi=10.1098%2frsos.161102&partnerID=40&md5=1eb0918e932ad5c3ec941bab9ab3c187
https://doi.org/10.1098/rsos.161102
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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