From laboratory to the field: Validating molecular markers of effect in Folsomia candida exposed to a fungicide-based formulation
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/101620 https://doi.org/10.1016/j.envint.2019.03.073 |
Resumo: | Under controlled laboratory conditions, toxicity data tend to be less variable than in more realistic in-field studies and responses may thus differ from those in the natural environment, creating uncertainty. The validation of data under environmental conditions is therefore a major asset in environmental risk assessment of chemicals. The present study aimed to validate the mode of action of a commercial fungicide formulation in the soil invertebrate F. candida, under more realistic exposure scenarios (in-field bioassay), by targeting specific molecular biomarkers retrieved from laboratory experiments. Organisms were exposed in soil cores under minimally controlled field conditions for 4 days to a chlorothalonil fungicide dosage causing 75% reduction of reproduction in a previous laboratory experiment (127 mg a.i. kg-1) and half this concentration (60 mg a.i. kg-1). After exposure, organisms were retrieved and RNA was extracted from each pool of organisms. According to previous laboratorial omics results with the same formulation, ten genes were selected for gene expression analysis by qRT-PCR, corresponding to key genes of affected biological pathways including glutathione metabolism, oxidation-reduction, body morphogenesis, and reproduction. Six of these genes presented a dose-response trend with higher up- or down-regulation with increasing pesticide concentrations. Highly significant correlations between their expression patterns in laboratory and in-field experiments were observed. This work shows that effects of toxicants can be clearly demonstrated in more realistic conditions using validated biomarkers. Our work outlines a set of genes that can be used to assess the early effects of pesticides in a realistic agricultural scenario. |
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From laboratory to the field: Validating molecular markers of effect in Folsomia candida exposed to a fungicide-based formulationBiomarkersChlorothalonilCollembolaFungicidesIn situ validationqPCRAnimalsArthropodsBiomarkersFungicides, IndustrialLaboratoriesNitrilesPesticidesReproductionSoilUnder controlled laboratory conditions, toxicity data tend to be less variable than in more realistic in-field studies and responses may thus differ from those in the natural environment, creating uncertainty. The validation of data under environmental conditions is therefore a major asset in environmental risk assessment of chemicals. The present study aimed to validate the mode of action of a commercial fungicide formulation in the soil invertebrate F. candida, under more realistic exposure scenarios (in-field bioassay), by targeting specific molecular biomarkers retrieved from laboratory experiments. Organisms were exposed in soil cores under minimally controlled field conditions for 4 days to a chlorothalonil fungicide dosage causing 75% reduction of reproduction in a previous laboratory experiment (127 mg a.i. kg-1) and half this concentration (60 mg a.i. kg-1). After exposure, organisms were retrieved and RNA was extracted from each pool of organisms. According to previous laboratorial omics results with the same formulation, ten genes were selected for gene expression analysis by qRT-PCR, corresponding to key genes of affected biological pathways including glutathione metabolism, oxidation-reduction, body morphogenesis, and reproduction. Six of these genes presented a dose-response trend with higher up- or down-regulation with increasing pesticide concentrations. Highly significant correlations between their expression patterns in laboratory and in-field experiments were observed. This work shows that effects of toxicants can be clearly demonstrated in more realistic conditions using validated biomarkers. Our work outlines a set of genes that can be used to assess the early effects of pesticides in a realistic agricultural scenario.2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/101620http://hdl.handle.net/10316/101620https://doi.org/10.1016/j.envint.2019.03.073eng01604120Simões, TiagoNovais, Sara CLuz, Tiago Natal daRenaud, MathieuLeston, SaraRamos, FernandoRömbke, JörgRoelofs, Dickvan Straalen, Nico MSousa, José P.Lemos, Marco Filipe Loureiroinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-09-05T20:43:25Zoai:estudogeral.uc.pt:10316/101620Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:18:46.538447Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
From laboratory to the field: Validating molecular markers of effect in Folsomia candida exposed to a fungicide-based formulation |
title |
From laboratory to the field: Validating molecular markers of effect in Folsomia candida exposed to a fungicide-based formulation |
spellingShingle |
From laboratory to the field: Validating molecular markers of effect in Folsomia candida exposed to a fungicide-based formulation Simões, Tiago Biomarkers Chlorothalonil Collembola Fungicides In situ validation qPCR Animals Arthropods Biomarkers Fungicides, Industrial Laboratories Nitriles Pesticides Reproduction Soil |
title_short |
From laboratory to the field: Validating molecular markers of effect in Folsomia candida exposed to a fungicide-based formulation |
title_full |
From laboratory to the field: Validating molecular markers of effect in Folsomia candida exposed to a fungicide-based formulation |
title_fullStr |
From laboratory to the field: Validating molecular markers of effect in Folsomia candida exposed to a fungicide-based formulation |
title_full_unstemmed |
From laboratory to the field: Validating molecular markers of effect in Folsomia candida exposed to a fungicide-based formulation |
title_sort |
From laboratory to the field: Validating molecular markers of effect in Folsomia candida exposed to a fungicide-based formulation |
author |
Simões, Tiago |
author_facet |
Simões, Tiago Novais, Sara C Luz, Tiago Natal da Renaud, Mathieu Leston, Sara Ramos, Fernando Römbke, Jörg Roelofs, Dick van Straalen, Nico M Sousa, José P. Lemos, Marco Filipe Loureiro |
author_role |
author |
author2 |
Novais, Sara C Luz, Tiago Natal da Renaud, Mathieu Leston, Sara Ramos, Fernando Römbke, Jörg Roelofs, Dick van Straalen, Nico M Sousa, José P. Lemos, Marco Filipe Loureiro |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Simões, Tiago Novais, Sara C Luz, Tiago Natal da Renaud, Mathieu Leston, Sara Ramos, Fernando Römbke, Jörg Roelofs, Dick van Straalen, Nico M Sousa, José P. Lemos, Marco Filipe Loureiro |
dc.subject.por.fl_str_mv |
Biomarkers Chlorothalonil Collembola Fungicides In situ validation qPCR Animals Arthropods Biomarkers Fungicides, Industrial Laboratories Nitriles Pesticides Reproduction Soil |
topic |
Biomarkers Chlorothalonil Collembola Fungicides In situ validation qPCR Animals Arthropods Biomarkers Fungicides, Industrial Laboratories Nitriles Pesticides Reproduction Soil |
description |
Under controlled laboratory conditions, toxicity data tend to be less variable than in more realistic in-field studies and responses may thus differ from those in the natural environment, creating uncertainty. The validation of data under environmental conditions is therefore a major asset in environmental risk assessment of chemicals. The present study aimed to validate the mode of action of a commercial fungicide formulation in the soil invertebrate F. candida, under more realistic exposure scenarios (in-field bioassay), by targeting specific molecular biomarkers retrieved from laboratory experiments. Organisms were exposed in soil cores under minimally controlled field conditions for 4 days to a chlorothalonil fungicide dosage causing 75% reduction of reproduction in a previous laboratory experiment (127 mg a.i. kg-1) and half this concentration (60 mg a.i. kg-1). After exposure, organisms were retrieved and RNA was extracted from each pool of organisms. According to previous laboratorial omics results with the same formulation, ten genes were selected for gene expression analysis by qRT-PCR, corresponding to key genes of affected biological pathways including glutathione metabolism, oxidation-reduction, body morphogenesis, and reproduction. Six of these genes presented a dose-response trend with higher up- or down-regulation with increasing pesticide concentrations. Highly significant correlations between their expression patterns in laboratory and in-field experiments were observed. This work shows that effects of toxicants can be clearly demonstrated in more realistic conditions using validated biomarkers. Our work outlines a set of genes that can be used to assess the early effects of pesticides in a realistic agricultural scenario. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/101620 http://hdl.handle.net/10316/101620 https://doi.org/10.1016/j.envint.2019.03.073 |
url |
http://hdl.handle.net/10316/101620 https://doi.org/10.1016/j.envint.2019.03.073 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
01604120 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799134082276261888 |