Imprinting fidelity in mouse iPSCs depends on sex of donor cell and medium formulation

• Autor(a) principal: Arez, Maria
• Data de Publicação: 2022
• Outros Autores: Eckersley-Maslin, Melanie, Klobučar, Tajda, von Gilsa Lopes, João, Krueger, Felix, Mupo, Annalisa, Raposo, Ana Cláudia, Oxley, David, Mancino, Samantha, Gendrel, Anne Valerie, Bernardes de Jesus, Bruno, da Rocha, Simão Teixeira
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/10362/144427
• topic: Chemistry(all); Biochemistry, Genetics and Molecular Biology(all); General; Physics and Astronomy(all)

Funding Information: We would like to thank Sérgio de Almeida, Miguel Casanova and Inês Milagre for critical reading of the manuscript, and the members of the S.T.d.R.’s team for helpful discussions. We also thank Tânia Carvalho and Pedro Ruivo for their help in histological analysis; Judith Webster at Babraham Institute for LC-MS measurements; Bethan Hussey at Sanger Sequencing and Kristina Tabbada at Babraham Institute for assistance with high-throughput sequencing; and the Bioimaging unit as well as Andreia Santos, Rute Gonçalves and Mariana Fernandes of the Flow Cytometry Facility of Instituto de Medicina Molecular João Lobo Antunes for their services and assistance. Work in S.T.d.R.’s team was supported by Fundação para a Ciência e Tecnologia (FCT) Ministério da Ciência, Tecnologia e Ensino Superior (MCTES), Portugal [IC&DT projects PTDC/BEX-BCM/2612/2014 and PTDC/BIA-MOL/29320/2017 as well as projects UIDB/04565/2020 and UIDP/04565/2020 of the Research Unit Institute from Bioengineering and Biosciences – iBB and LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy – i4HB]; S.T.d.R. and A.-V.G. are supported by assistant research contracts from FCT/MCTES (CEECIND/01234/2017 and CEECIND/02085/2018, respectively); M.A and A.C.R. are supported, respectively, by SFRH/BD/151251/2021 and SFRH/BD/137099/2018 PhD fellowships from FCT/MCTES. J.V.G.L is supported by COVID/BD/152624/2022 from FCT/MCTES. MAE-M was supported by a BBSRC Discovery Fellowship (BB/T009713/1) and is now supported by a Snow Medical Fellowship. F.K. is supported by the Babraham Institute Strategic Core Funding and A.M. by BBSRC BBS/E/B/000C0421. B.B.J. work was funded by Fundação para a Ciência e Tecnologia (FCT), and FEDER, LISBOA-01-0145-FEDER-028534, project co-funded by FEDER, through POR Lisboa 2020—Programa Operacional Regional de Lisboa. T.K. is supported by Janko Jamnik Doctoral Scholarship from National Institute of Chemistry. Funding Information: We would like to thank Sérgio de Almeida, Miguel Casanova and Inês Milagre for critical reading of the manuscript, and the members of the S.T.d.R.’s team for helpful discussions. We also thank Tânia Carvalho and Pedro Ruivo for their help in histological analysis; Judith Webster at Babraham Institute for LC-MS measurements; Bethan Hussey at Sanger Sequencing and Kristina Tabbada at Babraham Institute for assistance with high-throughput sequencing; and the Bioimaging unit as well as Andreia Santos, Rute Gonçalves and Mariana Fernandes of the Flow Cytometry Facility of Instituto de Medicina Molecular João Lobo Antunes for their services and assistance. Work in S.T.d.R.’s team was supported by Fundação para a Ciência e Tecnologia (FCT) Ministério da Ciência, Tecnologia e Ensino Superior (MCTES), Portugal [IC&DT projects PTDC/BEX-BCM/2612/2014 and PTDC/BIA-MOL/29320/2017 as well as projects UIDB/04565/2020 and UIDP/04565/2020 of the Research Unit Institute from Bioengineering and Biosciences – iBB and LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy – i4HB]; S.T.d.R. and A.-V.G. are supported by assistant research contracts from FCT/MCTES (CEECIND/01234/2017 and CEECIND/02085/2018, respectively); M.A and A.C.R. are supported, respectively, by SFRH/BD/151251/2021 and SFRH/BD/137099/2018 PhD fellowships from FCT/MCTES. J.V.G.L is supported by COVID/BD/152624/2022 from FCT/MCTES. MAE-M was supported by a BBSRC Discovery Fellowship (BB/T009713/1) and is now supported by a Snow Medical Fellowship. F.K. is supported by the Babraham Institute Strategic Core Funding and A.M. by BBSRC BBS/E/B/000C0421. B.B.J. work was funded by Fundação para a Ciência e Tecnologia (FCT), and FEDER, LISBOA-01-0145-FEDER-028534, project co-funded by FEDER, through POR Lisboa 2020—Programa Operacional Regional de Lisboa. T.K. is supported by Janko Jamnik Doctoral Scholarship from National Institute of Chemistry. Publisher Copyright: © 2022, The Author(s).