Applications of yeast flocculation in biotechnological processes

Detalhes bibliográficos
Autor(a) principal: Domingues, Lucília
Data de Publicação: 2000
Outros Autores: Vicente, A. A., Lima, Nelson, Teixeira, J. A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/1155
Resumo: A review on the main aspects associated with yeast flocculation and its application in biotechnological processes is presented. This subject is addressed following three main aspects – the basics of yeast flocculation, the development of “new” flocculating yeast strains and bioreactor development. In what concerns the basics of yeast flocculation, the state of the art on the most relevant aspects of mechanism, physiology and genetics of yeast flocculation is reported. The construction of flocculating yeast strains includes not only the recombinant constitutive flocculent brewer’s yeast, but also recombinant flocculent yeast for lactose metabolisation and ethanol production. Furthermore, recent work on the heterologous β-galactosidase production using a recombinant flocculent Saccharomyces cerevisiae is considered. As bioreactors using flocculating yeast cells have particular properties, mainly associated with a high solid phase hold-up, a section dedicated to its operation is presented. Aspects such as bioreactor productivity and culture stability as well as bioreactor hydrodynamics and mass transfer properties of flocculating cell cultures are considered. Finally, the paper concludes describing some of the applications of high cell density flocculation bioreactors and discussing potential new uses of these systems.
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spelling Applications of yeast flocculation in biotechnological processesRecombinant yeastFlocculationAirlift bioreactorHeterologous protein productionFermentationA review on the main aspects associated with yeast flocculation and its application in biotechnological processes is presented. This subject is addressed following three main aspects – the basics of yeast flocculation, the development of “new” flocculating yeast strains and bioreactor development. In what concerns the basics of yeast flocculation, the state of the art on the most relevant aspects of mechanism, physiology and genetics of yeast flocculation is reported. The construction of flocculating yeast strains includes not only the recombinant constitutive flocculent brewer’s yeast, but also recombinant flocculent yeast for lactose metabolisation and ethanol production. Furthermore, recent work on the heterologous β-galactosidase production using a recombinant flocculent Saccharomyces cerevisiae is considered. As bioreactors using flocculating yeast cells have particular properties, mainly associated with a high solid phase hold-up, a section dedicated to its operation is presented. Aspects such as bioreactor productivity and culture stability as well as bioreactor hydrodynamics and mass transfer properties of flocculating cell cultures are considered. Finally, the paper concludes describing some of the applications of high cell density flocculation bioreactors and discussing potential new uses of these systems.Fundação para a Ciência e a Tecnologia (FCT) – PRAXIS XXI - BD11306/97.Korean Society for Biotechnology and BioengineeringUniversidade do MinhoDomingues, LucíliaVicente, A. A.Lima, NelsonTeixeira, J. A.20002000-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/1155eng"Biotechnology & bioprocess engineering". ISSN 1226-8372. 5 (2000) 288-305.1226-837210.1007/BF02942185info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:33:02Zoai:repositorium.sdum.uminho.pt:1822/1155Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:28:29.582034Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Applications of yeast flocculation in biotechnological processes
title Applications of yeast flocculation in biotechnological processes
spellingShingle Applications of yeast flocculation in biotechnological processes
Domingues, Lucília
Recombinant yeast
Flocculation
Airlift bioreactor
Heterologous protein production
Fermentation
title_short Applications of yeast flocculation in biotechnological processes
title_full Applications of yeast flocculation in biotechnological processes
title_fullStr Applications of yeast flocculation in biotechnological processes
title_full_unstemmed Applications of yeast flocculation in biotechnological processes
title_sort Applications of yeast flocculation in biotechnological processes
author Domingues, Lucília
author_facet Domingues, Lucília
Vicente, A. A.
Lima, Nelson
Teixeira, J. A.
author_role author
author2 Vicente, A. A.
Lima, Nelson
Teixeira, J. A.
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Domingues, Lucília
Vicente, A. A.
Lima, Nelson
Teixeira, J. A.
dc.subject.por.fl_str_mv Recombinant yeast
Flocculation
Airlift bioreactor
Heterologous protein production
Fermentation
topic Recombinant yeast
Flocculation
Airlift bioreactor
Heterologous protein production
Fermentation
description A review on the main aspects associated with yeast flocculation and its application in biotechnological processes is presented. This subject is addressed following three main aspects – the basics of yeast flocculation, the development of “new” flocculating yeast strains and bioreactor development. In what concerns the basics of yeast flocculation, the state of the art on the most relevant aspects of mechanism, physiology and genetics of yeast flocculation is reported. The construction of flocculating yeast strains includes not only the recombinant constitutive flocculent brewer’s yeast, but also recombinant flocculent yeast for lactose metabolisation and ethanol production. Furthermore, recent work on the heterologous β-galactosidase production using a recombinant flocculent Saccharomyces cerevisiae is considered. As bioreactors using flocculating yeast cells have particular properties, mainly associated with a high solid phase hold-up, a section dedicated to its operation is presented. Aspects such as bioreactor productivity and culture stability as well as bioreactor hydrodynamics and mass transfer properties of flocculating cell cultures are considered. Finally, the paper concludes describing some of the applications of high cell density flocculation bioreactors and discussing potential new uses of these systems.
publishDate 2000
dc.date.none.fl_str_mv 2000
2000-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/1155
url http://hdl.handle.net/1822/1155
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv "Biotechnology & bioprocess engineering". ISSN 1226-8372. 5 (2000) 288-305.
1226-8372
10.1007/BF02942185
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Korean Society for Biotechnology and Bioengineering
publisher.none.fl_str_mv Korean Society for Biotechnology and Bioengineering
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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