Recombinant microbial systems for improved β-galactosidase production and biotechnological applications
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/22515 |
Resumo: | β-Galactosidases (EC 3.2.1.23) constitute a large family of proteins that are known to catalyze both hydrolytic and transgalactosylation reactions. The hydrolytic activity has been applied in the food industry for decades for reducing the lactose content in milk, while the transgalactosylation activity has been used to synthesize galacto-oligosaccharides and galactose containing chemicals in recent years. The main focus of this review is on the expression and production of Aspergillus niger, Kluyveromyces lactis and bacterial β-galactosidases in different microbial hosts. Furthermore, emphasis is given on the reported applications of the recombinant enzymes. Current developments on novel β-galactosidases, derived from newly identified microbial sources or by protein engineering means, together with the use of efficient recombinant microbial production systems are converting this enzyme into a relevant synthetic tool. Thermostable β-galactosidases (cold-adapted or thermophilic) in addition to the growing market for functional foods will likely redouble its industrial interest. |
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Recombinant microbial systems for improved β-galactosidase production and biotechnological applicationsRecombinant β-galactosidaseThermophilic enzymesCold-adapted enzymesRecombinant microbial systemsTransgalactosylation activityMilk lactose hydrolysisWhey bioremediationRecombinant beta-galactosidaseScience & Technologyβ-Galactosidases (EC 3.2.1.23) constitute a large family of proteins that are known to catalyze both hydrolytic and transgalactosylation reactions. The hydrolytic activity has been applied in the food industry for decades for reducing the lactose content in milk, while the transgalactosylation activity has been used to synthesize galacto-oligosaccharides and galactose containing chemicals in recent years. The main focus of this review is on the expression and production of Aspergillus niger, Kluyveromyces lactis and bacterial β-galactosidases in different microbial hosts. Furthermore, emphasis is given on the reported applications of the recombinant enzymes. Current developments on novel β-galactosidases, derived from newly identified microbial sources or by protein engineering means, together with the use of efficient recombinant microbial production systems are converting this enzyme into a relevant synthetic tool. Thermostable β-galactosidases (cold-adapted or thermophilic) in addition to the growing market for functional foods will likely redouble its industrial interest.C. Oliveira and P. M. R. Guimaraes acknowledge support from Fundacao para a Ciencia e a Tecnologia (FCT), Portugal (grants SFRH/BDP/63831/2009 and SFRH/BDP/44328/2008, respectively).ElsevierUniversidade do MinhoOliveira, Carla Cristina Marques deGuimarães, Pedro M. R.Domingues, Lucília20112011-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/22515eng0734-975010.1016/j.biotechadv.2011.03.00821514372http://www.sciencedirect.com/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:06:35Zoai:repositorium.sdum.uminho.pt:1822/22515Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:57:20.626428Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Recombinant microbial systems for improved β-galactosidase production and biotechnological applications |
title |
Recombinant microbial systems for improved β-galactosidase production and biotechnological applications |
spellingShingle |
Recombinant microbial systems for improved β-galactosidase production and biotechnological applications Oliveira, Carla Cristina Marques de Recombinant β-galactosidase Thermophilic enzymes Cold-adapted enzymes Recombinant microbial systems Transgalactosylation activity Milk lactose hydrolysis Whey bioremediation Recombinant beta-galactosidase Science & Technology |
title_short |
Recombinant microbial systems for improved β-galactosidase production and biotechnological applications |
title_full |
Recombinant microbial systems for improved β-galactosidase production and biotechnological applications |
title_fullStr |
Recombinant microbial systems for improved β-galactosidase production and biotechnological applications |
title_full_unstemmed |
Recombinant microbial systems for improved β-galactosidase production and biotechnological applications |
title_sort |
Recombinant microbial systems for improved β-galactosidase production and biotechnological applications |
author |
Oliveira, Carla Cristina Marques de |
author_facet |
Oliveira, Carla Cristina Marques de Guimarães, Pedro M. R. Domingues, Lucília |
author_role |
author |
author2 |
Guimarães, Pedro M. R. Domingues, Lucília |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Oliveira, Carla Cristina Marques de Guimarães, Pedro M. R. Domingues, Lucília |
dc.subject.por.fl_str_mv |
Recombinant β-galactosidase Thermophilic enzymes Cold-adapted enzymes Recombinant microbial systems Transgalactosylation activity Milk lactose hydrolysis Whey bioremediation Recombinant beta-galactosidase Science & Technology |
topic |
Recombinant β-galactosidase Thermophilic enzymes Cold-adapted enzymes Recombinant microbial systems Transgalactosylation activity Milk lactose hydrolysis Whey bioremediation Recombinant beta-galactosidase Science & Technology |
description |
β-Galactosidases (EC 3.2.1.23) constitute a large family of proteins that are known to catalyze both hydrolytic and transgalactosylation reactions. The hydrolytic activity has been applied in the food industry for decades for reducing the lactose content in milk, while the transgalactosylation activity has been used to synthesize galacto-oligosaccharides and galactose containing chemicals in recent years. The main focus of this review is on the expression and production of Aspergillus niger, Kluyveromyces lactis and bacterial β-galactosidases in different microbial hosts. Furthermore, emphasis is given on the reported applications of the recombinant enzymes. Current developments on novel β-galactosidases, derived from newly identified microbial sources or by protein engineering means, together with the use of efficient recombinant microbial production systems are converting this enzyme into a relevant synthetic tool. Thermostable β-galactosidases (cold-adapted or thermophilic) in addition to the growing market for functional foods will likely redouble its industrial interest. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011 2011-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/22515 |
url |
http://hdl.handle.net/1822/22515 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0734-9750 10.1016/j.biotechadv.2011.03.008 21514372 http://www.sciencedirect.com/ |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
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1799132362159685632 |