Bioactive properties of the medicinal mushroom Leucopaxillus giganteus mycelium obtained in the presence of different nitrogen sources

Detalhes bibliográficos
Autor(a) principal: Barros, Lillian
Data de Publicação: 2007
Outros Autores: Baptista, Paula, Estevinho, Leticia M., Ferreira, Isabel C.F.R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10198/833
Resumo: Leucopaxillus mushroom species are used in the chemical industry for extraction of clitocybin antibiotic and particularly the edible mushroom Leucopaxillus giganteus can be found in Northeast Portugal. Nevertheless, the production of its mycelium for pharmacological applications has not been explored. Herein, the mycelium obtained in the presence of four different nitrogen sources was investigated with regard to phenol production and antimicrobial and antioxidant properties. Phenol concentration increased along the growth time as a response to the oxidative stress and therefore free radical production. Although significant differences for mycelium growth between the nitrogen sources had not been observed, (NH4)2HPO4 proved to be the most appropriate to increase bioactive properties, leading to the highest phenol content and lowest EC50 and MIC values. The antimicrobial capacity was screened against Gram positive and Gram negative bacteria, and fungi. The samples selectively inhibited the growth of Gram positive bacteria, Staphylococcus aureus being the most susceptible one. Gram negative bacteria and fungi were resistant to the extracts. Antioxidant activity was calculated by the reducing power assay, the scavenging effect on DPPH radicals, and hemolysis and lipid peroxidation inhibition. Significantly negative linear regressions were established between phenols/flavonoids contents, which increased along the mycelia growth time and antioxidant activity.
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spelling Bioactive properties of the medicinal mushroom Leucopaxillus giganteus mycelium obtained in the presence of different nitrogen sourcesMushroom myceliumNitrogen sourceAntimicrobial activityAntioxidant potentialNutraceuticalsLeucopaxillus mushroom species are used in the chemical industry for extraction of clitocybin antibiotic and particularly the edible mushroom Leucopaxillus giganteus can be found in Northeast Portugal. Nevertheless, the production of its mycelium for pharmacological applications has not been explored. Herein, the mycelium obtained in the presence of four different nitrogen sources was investigated with regard to phenol production and antimicrobial and antioxidant properties. Phenol concentration increased along the growth time as a response to the oxidative stress and therefore free radical production. Although significant differences for mycelium growth between the nitrogen sources had not been observed, (NH4)2HPO4 proved to be the most appropriate to increase bioactive properties, leading to the highest phenol content and lowest EC50 and MIC values. The antimicrobial capacity was screened against Gram positive and Gram negative bacteria, and fungi. The samples selectively inhibited the growth of Gram positive bacteria, Staphylococcus aureus being the most susceptible one. Gram negative bacteria and fungi were resistant to the extracts. Antioxidant activity was calculated by the reducing power assay, the scavenging effect on DPPH radicals, and hemolysis and lipid peroxidation inhibition. Significantly negative linear regressions were established between phenols/flavonoids contents, which increased along the mycelia growth time and antioxidant activity.ElsevierBiblioteca Digital do IPBBarros, LillianBaptista, PaulaEstevinho, Leticia M.Ferreira, Isabel C.F.R.2008-09-12T15:40:20Z20072007-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/833engBarros, Lillian; Baptista, Paula; Estevinho, Letícia M.; Ferreira, Isabel C.F.R. (2007). Bioactive properties of the medicinal mushroom Leucopaxillus giganteus mycelium obtained in the presence of different nitrogen sources. Food Chemistry. ISSN 0308-8146. 105:1, p. 179-1860308-814610.1016/j.foodchem.2007.03.063info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:03:56Zoai:bibliotecadigital.ipb.pt:10198/833Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:54:28.303521Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Bioactive properties of the medicinal mushroom Leucopaxillus giganteus mycelium obtained in the presence of different nitrogen sources
title Bioactive properties of the medicinal mushroom Leucopaxillus giganteus mycelium obtained in the presence of different nitrogen sources
spellingShingle Bioactive properties of the medicinal mushroom Leucopaxillus giganteus mycelium obtained in the presence of different nitrogen sources
Barros, Lillian
Mushroom mycelium
Nitrogen source
Antimicrobial activity
Antioxidant potential
Nutraceuticals
title_short Bioactive properties of the medicinal mushroom Leucopaxillus giganteus mycelium obtained in the presence of different nitrogen sources
title_full Bioactive properties of the medicinal mushroom Leucopaxillus giganteus mycelium obtained in the presence of different nitrogen sources
title_fullStr Bioactive properties of the medicinal mushroom Leucopaxillus giganteus mycelium obtained in the presence of different nitrogen sources
title_full_unstemmed Bioactive properties of the medicinal mushroom Leucopaxillus giganteus mycelium obtained in the presence of different nitrogen sources
title_sort Bioactive properties of the medicinal mushroom Leucopaxillus giganteus mycelium obtained in the presence of different nitrogen sources
author Barros, Lillian
author_facet Barros, Lillian
Baptista, Paula
Estevinho, Leticia M.
Ferreira, Isabel C.F.R.
author_role author
author2 Baptista, Paula
Estevinho, Leticia M.
Ferreira, Isabel C.F.R.
author2_role author
author
author
dc.contributor.none.fl_str_mv Biblioteca Digital do IPB
dc.contributor.author.fl_str_mv Barros, Lillian
Baptista, Paula
Estevinho, Leticia M.
Ferreira, Isabel C.F.R.
dc.subject.por.fl_str_mv Mushroom mycelium
Nitrogen source
Antimicrobial activity
Antioxidant potential
Nutraceuticals
topic Mushroom mycelium
Nitrogen source
Antimicrobial activity
Antioxidant potential
Nutraceuticals
description Leucopaxillus mushroom species are used in the chemical industry for extraction of clitocybin antibiotic and particularly the edible mushroom Leucopaxillus giganteus can be found in Northeast Portugal. Nevertheless, the production of its mycelium for pharmacological applications has not been explored. Herein, the mycelium obtained in the presence of four different nitrogen sources was investigated with regard to phenol production and antimicrobial and antioxidant properties. Phenol concentration increased along the growth time as a response to the oxidative stress and therefore free radical production. Although significant differences for mycelium growth between the nitrogen sources had not been observed, (NH4)2HPO4 proved to be the most appropriate to increase bioactive properties, leading to the highest phenol content and lowest EC50 and MIC values. The antimicrobial capacity was screened against Gram positive and Gram negative bacteria, and fungi. The samples selectively inhibited the growth of Gram positive bacteria, Staphylococcus aureus being the most susceptible one. Gram negative bacteria and fungi were resistant to the extracts. Antioxidant activity was calculated by the reducing power assay, the scavenging effect on DPPH radicals, and hemolysis and lipid peroxidation inhibition. Significantly negative linear regressions were established between phenols/flavonoids contents, which increased along the mycelia growth time and antioxidant activity.
publishDate 2007
dc.date.none.fl_str_mv 2007
2007-01-01T00:00:00Z
2008-09-12T15:40:20Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10198/833
url http://hdl.handle.net/10198/833
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Barros, Lillian; Baptista, Paula; Estevinho, Letícia M.; Ferreira, Isabel C.F.R. (2007). Bioactive properties of the medicinal mushroom Leucopaxillus giganteus mycelium obtained in the presence of different nitrogen sources. Food Chemistry. ISSN 0308-8146. 105:1, p. 179-186
0308-8146
10.1016/j.foodchem.2007.03.063
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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