Bioactive properties of the medicinal mushroom Leucopaxillus giganteus mycelium obtained in the presence of different nitrogen sources
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10198/833 |
Resumo: | Leucopaxillus mushroom species are used in the chemical industry for extraction of clitocybin antibiotic and particularly the edible mushroom Leucopaxillus giganteus can be found in Northeast Portugal. Nevertheless, the production of its mycelium for pharmacological applications has not been explored. Herein, the mycelium obtained in the presence of four different nitrogen sources was investigated with regard to phenol production and antimicrobial and antioxidant properties. Phenol concentration increased along the growth time as a response to the oxidative stress and therefore free radical production. Although significant differences for mycelium growth between the nitrogen sources had not been observed, (NH4)2HPO4 proved to be the most appropriate to increase bioactive properties, leading to the highest phenol content and lowest EC50 and MIC values. The antimicrobial capacity was screened against Gram positive and Gram negative bacteria, and fungi. The samples selectively inhibited the growth of Gram positive bacteria, Staphylococcus aureus being the most susceptible one. Gram negative bacteria and fungi were resistant to the extracts. Antioxidant activity was calculated by the reducing power assay, the scavenging effect on DPPH radicals, and hemolysis and lipid peroxidation inhibition. Significantly negative linear regressions were established between phenols/flavonoids contents, which increased along the mycelia growth time and antioxidant activity. |
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Bioactive properties of the medicinal mushroom Leucopaxillus giganteus mycelium obtained in the presence of different nitrogen sourcesMushroom myceliumNitrogen sourceAntimicrobial activityAntioxidant potentialNutraceuticalsLeucopaxillus mushroom species are used in the chemical industry for extraction of clitocybin antibiotic and particularly the edible mushroom Leucopaxillus giganteus can be found in Northeast Portugal. Nevertheless, the production of its mycelium for pharmacological applications has not been explored. Herein, the mycelium obtained in the presence of four different nitrogen sources was investigated with regard to phenol production and antimicrobial and antioxidant properties. Phenol concentration increased along the growth time as a response to the oxidative stress and therefore free radical production. Although significant differences for mycelium growth between the nitrogen sources had not been observed, (NH4)2HPO4 proved to be the most appropriate to increase bioactive properties, leading to the highest phenol content and lowest EC50 and MIC values. The antimicrobial capacity was screened against Gram positive and Gram negative bacteria, and fungi. The samples selectively inhibited the growth of Gram positive bacteria, Staphylococcus aureus being the most susceptible one. Gram negative bacteria and fungi were resistant to the extracts. Antioxidant activity was calculated by the reducing power assay, the scavenging effect on DPPH radicals, and hemolysis and lipid peroxidation inhibition. Significantly negative linear regressions were established between phenols/flavonoids contents, which increased along the mycelia growth time and antioxidant activity.ElsevierBiblioteca Digital do IPBBarros, LillianBaptista, PaulaEstevinho, Leticia M.Ferreira, Isabel C.F.R.2008-09-12T15:40:20Z20072007-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/833engBarros, Lillian; Baptista, Paula; Estevinho, Letícia M.; Ferreira, Isabel C.F.R. (2007). Bioactive properties of the medicinal mushroom Leucopaxillus giganteus mycelium obtained in the presence of different nitrogen sources. Food Chemistry. ISSN 0308-8146. 105:1, p. 179-1860308-814610.1016/j.foodchem.2007.03.063info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:03:56Zoai:bibliotecadigital.ipb.pt:10198/833Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:54:28.303521Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Bioactive properties of the medicinal mushroom Leucopaxillus giganteus mycelium obtained in the presence of different nitrogen sources |
title |
Bioactive properties of the medicinal mushroom Leucopaxillus giganteus mycelium obtained in the presence of different nitrogen sources |
spellingShingle |
Bioactive properties of the medicinal mushroom Leucopaxillus giganteus mycelium obtained in the presence of different nitrogen sources Barros, Lillian Mushroom mycelium Nitrogen source Antimicrobial activity Antioxidant potential Nutraceuticals |
title_short |
Bioactive properties of the medicinal mushroom Leucopaxillus giganteus mycelium obtained in the presence of different nitrogen sources |
title_full |
Bioactive properties of the medicinal mushroom Leucopaxillus giganteus mycelium obtained in the presence of different nitrogen sources |
title_fullStr |
Bioactive properties of the medicinal mushroom Leucopaxillus giganteus mycelium obtained in the presence of different nitrogen sources |
title_full_unstemmed |
Bioactive properties of the medicinal mushroom Leucopaxillus giganteus mycelium obtained in the presence of different nitrogen sources |
title_sort |
Bioactive properties of the medicinal mushroom Leucopaxillus giganteus mycelium obtained in the presence of different nitrogen sources |
author |
Barros, Lillian |
author_facet |
Barros, Lillian Baptista, Paula Estevinho, Leticia M. Ferreira, Isabel C.F.R. |
author_role |
author |
author2 |
Baptista, Paula Estevinho, Leticia M. Ferreira, Isabel C.F.R. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Biblioteca Digital do IPB |
dc.contributor.author.fl_str_mv |
Barros, Lillian Baptista, Paula Estevinho, Leticia M. Ferreira, Isabel C.F.R. |
dc.subject.por.fl_str_mv |
Mushroom mycelium Nitrogen source Antimicrobial activity Antioxidant potential Nutraceuticals |
topic |
Mushroom mycelium Nitrogen source Antimicrobial activity Antioxidant potential Nutraceuticals |
description |
Leucopaxillus mushroom species are used in the chemical industry for extraction of clitocybin antibiotic and particularly the edible mushroom Leucopaxillus giganteus can be found in Northeast Portugal. Nevertheless, the production of its mycelium for pharmacological applications has not been explored. Herein, the mycelium obtained in the presence of four different nitrogen sources was investigated with regard to phenol production and antimicrobial and antioxidant properties. Phenol concentration increased along the growth time as a response to the oxidative stress and therefore free radical production. Although significant differences for mycelium growth between the nitrogen sources had not been observed, (NH4)2HPO4 proved to be the most appropriate to increase bioactive properties, leading to the highest phenol content and lowest EC50 and MIC values. The antimicrobial capacity was screened against Gram positive and Gram negative bacteria, and fungi. The samples selectively inhibited the growth of Gram positive bacteria, Staphylococcus aureus being the most susceptible one. Gram negative bacteria and fungi were resistant to the extracts. Antioxidant activity was calculated by the reducing power assay, the scavenging effect on DPPH radicals, and hemolysis and lipid peroxidation inhibition. Significantly negative linear regressions were established between phenols/flavonoids contents, which increased along the mycelia growth time and antioxidant activity. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007 2007-01-01T00:00:00Z 2008-09-12T15:40:20Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10198/833 |
url |
http://hdl.handle.net/10198/833 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Barros, Lillian; Baptista, Paula; Estevinho, Letícia M.; Ferreira, Isabel C.F.R. (2007). Bioactive properties of the medicinal mushroom Leucopaxillus giganteus mycelium obtained in the presence of different nitrogen sources. Food Chemistry. ISSN 0308-8146. 105:1, p. 179-186 0308-8146 10.1016/j.foodchem.2007.03.063 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799135143557857280 |