Olfactory sensitivity of the marine flatfish Solea senegalensis to conspecific body fluids
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.1/11536 |
Resumo: | Chemical communication is better understood in freshwater fish than marine fish. The Senegalese sole (Solea senegalensis) is a marine flatfish wherein one of the problems in aquaculture is the poor reproductive performance of hatchery-bred males. Is chemical communication involved in the reproduction of this species? Urine, intestinal fluid and mucus samples were taken from adult fish (wild-caught and hatchery-bred) over the spawning season (March-May), and assessed for olfactory potency using the electro-olfactogram (EOG). The effect of stimulation of the olfactory system with adult female urine on circulating luteinizing hormone (LH) levels was also tested in males. Intestinal fluid and urine were potent olfactory stimuli for both juvenile and adult conspecifics, evoking large-amplitude, concentration-dependent EOG responses, with thresholds of detection at approximately 1:10(6). However, the amplitude of the response to urine depended on the sex and state of maturity of both the donor and the receiver. Most olfactory activity could be extracted by C18 solid-phase cartridges. Urine from mature females evoked a slight, but significant, increase in circulating LH levels in mature males 30 min after exposure. Furthermore, the olfactory potency of urine differed between wild-caught and hatchery-bred fish; however, contrary to expectations, urine from wild-caught females was less potent than that from hatchery-bred females. Taken together, these results strongly suggest that faeces-and urine-released odorants are involved in reproduction in the Senegalese sole, and establish a basis for further investigation into pheromonal communication in marine teleosts. |
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Olfactory sensitivity of the marine flatfish Solea senegalensis to conspecific body fluidsEel Anguilla-AnguillaTilapia Oreochromis-MossambicusOvarian fluidSex-PheromoneOvulated femaleElectro-olfactogramSpawning readinessCarassius-AuratusSperm motilityMate selectionChemical communication is better understood in freshwater fish than marine fish. The Senegalese sole (Solea senegalensis) is a marine flatfish wherein one of the problems in aquaculture is the poor reproductive performance of hatchery-bred males. Is chemical communication involved in the reproduction of this species? Urine, intestinal fluid and mucus samples were taken from adult fish (wild-caught and hatchery-bred) over the spawning season (March-May), and assessed for olfactory potency using the electro-olfactogram (EOG). The effect of stimulation of the olfactory system with adult female urine on circulating luteinizing hormone (LH) levels was also tested in males. Intestinal fluid and urine were potent olfactory stimuli for both juvenile and adult conspecifics, evoking large-amplitude, concentration-dependent EOG responses, with thresholds of detection at approximately 1:10(6). However, the amplitude of the response to urine depended on the sex and state of maturity of both the donor and the receiver. Most olfactory activity could be extracted by C18 solid-phase cartridges. Urine from mature females evoked a slight, but significant, increase in circulating LH levels in mature males 30 min after exposure. Furthermore, the olfactory potency of urine differed between wild-caught and hatchery-bred fish; however, contrary to expectations, urine from wild-caught females was less potent than that from hatchery-bred females. Taken together, these results strongly suggest that faeces-and urine-released odorants are involved in reproduction in the Senegalese sole, and establish a basis for further investigation into pheromonal communication in marine teleosts.Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria; EU through FEDER [RTA2011-00050, RTA2014-00048]; Ministerio de Economia y Competitividad (MINECO) [AGL2013-41196-R, RTA2013-00023-C02-01]; FPI-INIA - MINECO, Spain; Fundacao para a Ciencia e a Tecnologia (Foundation for Science and Technology, Portugal) [UID/Multi/04326/2013]Company Of Biologists LtdSapientiaFatsini, ElviraCarazo, IgnacioChauvigne, FrancoisManchado, ManuelCerda, JoanHubbard, PeterDuncan, Neil J.2018-12-07T14:53:29Z2017-062017-06-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/11536eng0022-094910.1242/jeb.150318info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:23:21Zoai:sapientia.ualg.pt:10400.1/11536Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:03:01.829270Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Olfactory sensitivity of the marine flatfish Solea senegalensis to conspecific body fluids |
title |
Olfactory sensitivity of the marine flatfish Solea senegalensis to conspecific body fluids |
spellingShingle |
Olfactory sensitivity of the marine flatfish Solea senegalensis to conspecific body fluids Fatsini, Elvira Eel Anguilla-Anguilla Tilapia Oreochromis-Mossambicus Ovarian fluid Sex-Pheromone Ovulated female Electro-olfactogram Spawning readiness Carassius-Auratus Sperm motility Mate selection |
title_short |
Olfactory sensitivity of the marine flatfish Solea senegalensis to conspecific body fluids |
title_full |
Olfactory sensitivity of the marine flatfish Solea senegalensis to conspecific body fluids |
title_fullStr |
Olfactory sensitivity of the marine flatfish Solea senegalensis to conspecific body fluids |
title_full_unstemmed |
Olfactory sensitivity of the marine flatfish Solea senegalensis to conspecific body fluids |
title_sort |
Olfactory sensitivity of the marine flatfish Solea senegalensis to conspecific body fluids |
author |
Fatsini, Elvira |
author_facet |
Fatsini, Elvira Carazo, Ignacio Chauvigne, Francois Manchado, Manuel Cerda, Joan Hubbard, Peter Duncan, Neil J. |
author_role |
author |
author2 |
Carazo, Ignacio Chauvigne, Francois Manchado, Manuel Cerda, Joan Hubbard, Peter Duncan, Neil J. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Sapientia |
dc.contributor.author.fl_str_mv |
Fatsini, Elvira Carazo, Ignacio Chauvigne, Francois Manchado, Manuel Cerda, Joan Hubbard, Peter Duncan, Neil J. |
dc.subject.por.fl_str_mv |
Eel Anguilla-Anguilla Tilapia Oreochromis-Mossambicus Ovarian fluid Sex-Pheromone Ovulated female Electro-olfactogram Spawning readiness Carassius-Auratus Sperm motility Mate selection |
topic |
Eel Anguilla-Anguilla Tilapia Oreochromis-Mossambicus Ovarian fluid Sex-Pheromone Ovulated female Electro-olfactogram Spawning readiness Carassius-Auratus Sperm motility Mate selection |
description |
Chemical communication is better understood in freshwater fish than marine fish. The Senegalese sole (Solea senegalensis) is a marine flatfish wherein one of the problems in aquaculture is the poor reproductive performance of hatchery-bred males. Is chemical communication involved in the reproduction of this species? Urine, intestinal fluid and mucus samples were taken from adult fish (wild-caught and hatchery-bred) over the spawning season (March-May), and assessed for olfactory potency using the electro-olfactogram (EOG). The effect of stimulation of the olfactory system with adult female urine on circulating luteinizing hormone (LH) levels was also tested in males. Intestinal fluid and urine were potent olfactory stimuli for both juvenile and adult conspecifics, evoking large-amplitude, concentration-dependent EOG responses, with thresholds of detection at approximately 1:10(6). However, the amplitude of the response to urine depended on the sex and state of maturity of both the donor and the receiver. Most olfactory activity could be extracted by C18 solid-phase cartridges. Urine from mature females evoked a slight, but significant, increase in circulating LH levels in mature males 30 min after exposure. Furthermore, the olfactory potency of urine differed between wild-caught and hatchery-bred fish; however, contrary to expectations, urine from wild-caught females was less potent than that from hatchery-bred females. Taken together, these results strongly suggest that faeces-and urine-released odorants are involved in reproduction in the Senegalese sole, and establish a basis for further investigation into pheromonal communication in marine teleosts. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-06 2017-06-01T00:00:00Z 2018-12-07T14:53:29Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.1/11536 |
url |
http://hdl.handle.net/10400.1/11536 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0022-0949 10.1242/jeb.150318 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Company Of Biologists Ltd |
publisher.none.fl_str_mv |
Company Of Biologists Ltd |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799133264384884736 |