International external quality control assessment for the serological diagnosis of dengue infections

Detalhes bibliográficos
Autor(a) principal: Domingo, C.
Data de Publicação: 2015
Outros Autores: Alves, M.J., de Ory, F., Teichmann, A., Schmitz, H., Müller, R., Niedrig, M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.18/3340
Resumo: Acessível em: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4392463/
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spelling International external quality control assessment for the serological diagnosis of dengue infectionsInfecções Sistémicas e ZoonosesSerological DiagnosisDengue InfectionsAcessível em: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4392463/BACKGROUND: Dengue is endemic to the tropics and subtropics, and the most frequent of arthropod-borne viral diseases. Reliable diagnosis of dengue infection is important not only in clinical care but also in disease surveillance, the control of outbreaks, and the development of new vaccines. The diagnosis of dengue infection is usually established by a variety of commercial or in-house serological protocols. The European Network for the Diagnostics of Imported Viral Diseases (ENIVD) recognized the need to survey the accuracy of dengue serological diagnostics in current use, and organized an external quality assurance (EQA) study of dengue serological practice in diagnostic laboratories. METHODS: A 15-sample panel, consisting of sera reactive against dengue plus specificity and negative controls, was sent to 48 laboratories for serological testing. The results returned by the participating laboratories were anonymized, scored, and subjected to comparison and statistical analysis. RESULTS: Ten laboratories rated all samples correctly with regard to IgM, and only three achieved the full score for IgG detection. The main handicaps in assay performance were suboptimal sensitivity of in-house IgM detection protocols by comparison with better-performing commercial ELISA tests, and the presence of IgG cross-reactivity with heterologous flaviviruses. Differences of detail in the methodology of dengue IgG antibody detection appear to underlie the disparities in accuracy observed between laboratories. CONCLUSION: This EQA study demonstrates that there is room for many laboratories to improve sensitivity in the detection of anti-dengue virus IgM antibodies, against the benchmark set by commercial antibody capture ELISA tests. The EQA shows also that cross-reactivity is a continuing issue, and IgG detection protocols must be optimized to increase their specificity.Repositório Científico do Instituto Nacional de SaúdeDomingo, C.Alves, M.J.de Ory, F.Teichmann, A.Schmitz, H.Müller, R.Niedrig, M.2016-02-16T16:12:45Z2015-04-012015-04-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/3340engBMC Infect Dis. 2015 Apr 1;15:167. doi: 10.1186/s12879-015-0877-0.1471-233410.1186/s12879-015-0877-0info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:39:34Zoai:repositorio.insa.pt:10400.18/3340Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:37:58.304242Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv International external quality control assessment for the serological diagnosis of dengue infections
title International external quality control assessment for the serological diagnosis of dengue infections
spellingShingle International external quality control assessment for the serological diagnosis of dengue infections
Domingo, C.
Infecções Sistémicas e Zoonoses
Serological Diagnosis
Dengue Infections
title_short International external quality control assessment for the serological diagnosis of dengue infections
title_full International external quality control assessment for the serological diagnosis of dengue infections
title_fullStr International external quality control assessment for the serological diagnosis of dengue infections
title_full_unstemmed International external quality control assessment for the serological diagnosis of dengue infections
title_sort International external quality control assessment for the serological diagnosis of dengue infections
author Domingo, C.
author_facet Domingo, C.
Alves, M.J.
de Ory, F.
Teichmann, A.
Schmitz, H.
Müller, R.
Niedrig, M.
author_role author
author2 Alves, M.J.
de Ory, F.
Teichmann, A.
Schmitz, H.
Müller, R.
Niedrig, M.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Nacional de Saúde
dc.contributor.author.fl_str_mv Domingo, C.
Alves, M.J.
de Ory, F.
Teichmann, A.
Schmitz, H.
Müller, R.
Niedrig, M.
dc.subject.por.fl_str_mv Infecções Sistémicas e Zoonoses
Serological Diagnosis
Dengue Infections
topic Infecções Sistémicas e Zoonoses
Serological Diagnosis
Dengue Infections
description Acessível em: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4392463/
publishDate 2015
dc.date.none.fl_str_mv 2015-04-01
2015-04-01T00:00:00Z
2016-02-16T16:12:45Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.18/3340
url http://hdl.handle.net/10400.18/3340
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv BMC Infect Dis. 2015 Apr 1;15:167. doi: 10.1186/s12879-015-0877-0.
1471-2334
10.1186/s12879-015-0877-0
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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