DNA-based biosensor for the electrocatalytic determination of antioxidant capacity in beverages
Autor(a) principal: | |
---|---|
Data de Publicação: | 2011 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.22/2984 |
Resumo: | Reactive oxygen species (ROS) are produced as a consequence of normal aerobic metabolism and are able to induce DNA oxidative damage. At the cellular level, the evaluation of the protective effect of antioxidants can be achieved by examining the integrity of the DNA nucleobases using electrochemical techniques. Herein, the use of an adenine-rich oligonucleotide (dA21) adsorbed on carbon paste electrodes for the assessment of the antioxidant capacity is proposed. The method was based on the partial damage of a DNA layer adsorbed on the electrode surface by OH• radicals generated by Fenton reaction and the subsequent electrochemical oxidation of the intact adenine bases to generate an oxidation product that was able to catalyze the oxidation of NADH. The presence of antioxidant compounds scavenged hydroxyl radicals leaving more adenines unoxidized, and thus, increasing the electrocatalytic current of NADHmeasured by differential pulse voltammetry (DPV). Using ascorbic acid (AA) as a model antioxidant species, the detection of as low as 50nMof AA in aqueous solution was possible. The protection efficiency was evaluated for several antioxidant compounds. The biosensor was applied to the determination of the total antioxidant capacity (TAC) in beverages. |
id |
RCAP_5b15ace6e9e4f14fa5557abfe000500c |
---|---|
oai_identifier_str |
oai:recipp.ipp.pt:10400.22/2984 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
DNA-based biosensor for the electrocatalytic determination of antioxidant capacity in beveragesNADHDNA biosensorElectrocatalytic oxidationTotal antioxidant capacity (TAC)Ascorbic acidReactive oxygen species (ROS)Reactive oxygen species (ROS) are produced as a consequence of normal aerobic metabolism and are able to induce DNA oxidative damage. At the cellular level, the evaluation of the protective effect of antioxidants can be achieved by examining the integrity of the DNA nucleobases using electrochemical techniques. Herein, the use of an adenine-rich oligonucleotide (dA21) adsorbed on carbon paste electrodes for the assessment of the antioxidant capacity is proposed. The method was based on the partial damage of a DNA layer adsorbed on the electrode surface by OH• radicals generated by Fenton reaction and the subsequent electrochemical oxidation of the intact adenine bases to generate an oxidation product that was able to catalyze the oxidation of NADH. The presence of antioxidant compounds scavenged hydroxyl radicals leaving more adenines unoxidized, and thus, increasing the electrocatalytic current of NADHmeasured by differential pulse voltammetry (DPV). Using ascorbic acid (AA) as a model antioxidant species, the detection of as low as 50nMof AA in aqueous solution was possible. The protection efficiency was evaluated for several antioxidant compounds. The biosensor was applied to the determination of the total antioxidant capacity (TAC) in beverages.ElsevierRepositório Científico do Instituto Politécnico do PortoBarroso, M. Fátimade-los-Santos-Álvarez, N.Lobo-Castañón, M. J.Miranda-Ordieres, A. J.Delerue-Matos, CristinaOliveira, M. Beatriz P. P.Tuñón-Blanco, P.2013-11-27T12:12:16Z20112011-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.22/2984eng0956-566310.1016/j.bios.2010.10.019info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-13T12:42:39Zoai:recipp.ipp.pt:10400.22/2984Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:24:00.321715Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
DNA-based biosensor for the electrocatalytic determination of antioxidant capacity in beverages |
title |
DNA-based biosensor for the electrocatalytic determination of antioxidant capacity in beverages |
spellingShingle |
DNA-based biosensor for the electrocatalytic determination of antioxidant capacity in beverages Barroso, M. Fátima NADH DNA biosensor Electrocatalytic oxidation Total antioxidant capacity (TAC) Ascorbic acid Reactive oxygen species (ROS) |
title_short |
DNA-based biosensor for the electrocatalytic determination of antioxidant capacity in beverages |
title_full |
DNA-based biosensor for the electrocatalytic determination of antioxidant capacity in beverages |
title_fullStr |
DNA-based biosensor for the electrocatalytic determination of antioxidant capacity in beverages |
title_full_unstemmed |
DNA-based biosensor for the electrocatalytic determination of antioxidant capacity in beverages |
title_sort |
DNA-based biosensor for the electrocatalytic determination of antioxidant capacity in beverages |
author |
Barroso, M. Fátima |
author_facet |
Barroso, M. Fátima de-los-Santos-Álvarez, N. Lobo-Castañón, M. J. Miranda-Ordieres, A. J. Delerue-Matos, Cristina Oliveira, M. Beatriz P. P. Tuñón-Blanco, P. |
author_role |
author |
author2 |
de-los-Santos-Álvarez, N. Lobo-Castañón, M. J. Miranda-Ordieres, A. J. Delerue-Matos, Cristina Oliveira, M. Beatriz P. P. Tuñón-Blanco, P. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Politécnico do Porto |
dc.contributor.author.fl_str_mv |
Barroso, M. Fátima de-los-Santos-Álvarez, N. Lobo-Castañón, M. J. Miranda-Ordieres, A. J. Delerue-Matos, Cristina Oliveira, M. Beatriz P. P. Tuñón-Blanco, P. |
dc.subject.por.fl_str_mv |
NADH DNA biosensor Electrocatalytic oxidation Total antioxidant capacity (TAC) Ascorbic acid Reactive oxygen species (ROS) |
topic |
NADH DNA biosensor Electrocatalytic oxidation Total antioxidant capacity (TAC) Ascorbic acid Reactive oxygen species (ROS) |
description |
Reactive oxygen species (ROS) are produced as a consequence of normal aerobic metabolism and are able to induce DNA oxidative damage. At the cellular level, the evaluation of the protective effect of antioxidants can be achieved by examining the integrity of the DNA nucleobases using electrochemical techniques. Herein, the use of an adenine-rich oligonucleotide (dA21) adsorbed on carbon paste electrodes for the assessment of the antioxidant capacity is proposed. The method was based on the partial damage of a DNA layer adsorbed on the electrode surface by OH• radicals generated by Fenton reaction and the subsequent electrochemical oxidation of the intact adenine bases to generate an oxidation product that was able to catalyze the oxidation of NADH. The presence of antioxidant compounds scavenged hydroxyl radicals leaving more adenines unoxidized, and thus, increasing the electrocatalytic current of NADHmeasured by differential pulse voltammetry (DPV). Using ascorbic acid (AA) as a model antioxidant species, the detection of as low as 50nMof AA in aqueous solution was possible. The protection efficiency was evaluated for several antioxidant compounds. The biosensor was applied to the determination of the total antioxidant capacity (TAC) in beverages. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011 2011-01-01T00:00:00Z 2013-11-27T12:12:16Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.22/2984 |
url |
http://hdl.handle.net/10400.22/2984 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0956-5663 10.1016/j.bios.2010.10.019 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799131334741852160 |