Electrocatalytic evaluation of DNA damage by superoxide radical for antioxidant capacity assessment
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.22/3008 |
Resumo: | The integrity of DNA purine bases was herein used to evaluate the antioxidant capacity. Unlike other DNA-based antioxidant sensors reported so far, the damaging agent chosen was the O 2 radical enzymatically generated by the xanthine/xanthine oxidase system. An adenine-rich oligonucleotide was adsorbed on carbon paste electrodes and subjected to radical damage in the presence/absence of several antioxidant compounds. As a result, partial damage on DNA was observed. A minor product of the radical oxidation was identified by cyclic voltammetry as a diimine adenine derivative also formed during the electrochemical oxidation of adenine/guanine bases. The protective efficiency of several antioxidant compounds was evaluated after electrochemical oxidation of the remaining unoxidized adenine bases, by measuring the electrocatalytic current of NADH mediated by the adsorbed catalyst species generated. A comparison between O 2 and OH radicals as a source of DNA lesions and the scavenging efficiency of various antioxidant compounds against both of them is discussed. Finally, the antioxidant capacity of beverages was evaluated and compared with the results obtained with an optical method. |
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Electrocatalytic evaluation of DNA damage by superoxide radical for antioxidant capacity assessmentNADH electrocatalysisDNA damageAntioxidant capacityAscorbic acidReactive oxygen speciesSuperoxide radicalThe integrity of DNA purine bases was herein used to evaluate the antioxidant capacity. Unlike other DNA-based antioxidant sensors reported so far, the damaging agent chosen was the O 2 radical enzymatically generated by the xanthine/xanthine oxidase system. An adenine-rich oligonucleotide was adsorbed on carbon paste electrodes and subjected to radical damage in the presence/absence of several antioxidant compounds. As a result, partial damage on DNA was observed. A minor product of the radical oxidation was identified by cyclic voltammetry as a diimine adenine derivative also formed during the electrochemical oxidation of adenine/guanine bases. The protective efficiency of several antioxidant compounds was evaluated after electrochemical oxidation of the remaining unoxidized adenine bases, by measuring the electrocatalytic current of NADH mediated by the adsorbed catalyst species generated. A comparison between O 2 and OH radicals as a source of DNA lesions and the scavenging efficiency of various antioxidant compounds against both of them is discussed. Finally, the antioxidant capacity of beverages was evaluated and compared with the results obtained with an optical method.ElsevierRepositório Científico do Instituto Politécnico do PortoBarroso, M. Fátimade-los-Santos-Álvarez, N.Lobo-Castañón, M. J.Miranda-Ordieres, A. J.Delerue-Matos, CristinaOliveira, M. Beatriz P. P.Tuñón-Blanco, P.2013-11-27T17:01:42Z20112011-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.22/3008eng1572-665710.1016/j.jelechem.2011.04.022info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-13T12:42:43Zoai:recipp.ipp.pt:10400.22/3008Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:24:02.216804Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Electrocatalytic evaluation of DNA damage by superoxide radical for antioxidant capacity assessment |
title |
Electrocatalytic evaluation of DNA damage by superoxide radical for antioxidant capacity assessment |
spellingShingle |
Electrocatalytic evaluation of DNA damage by superoxide radical for antioxidant capacity assessment Barroso, M. Fátima NADH electrocatalysis DNA damage Antioxidant capacity Ascorbic acid Reactive oxygen species Superoxide radical |
title_short |
Electrocatalytic evaluation of DNA damage by superoxide radical for antioxidant capacity assessment |
title_full |
Electrocatalytic evaluation of DNA damage by superoxide radical for antioxidant capacity assessment |
title_fullStr |
Electrocatalytic evaluation of DNA damage by superoxide radical for antioxidant capacity assessment |
title_full_unstemmed |
Electrocatalytic evaluation of DNA damage by superoxide radical for antioxidant capacity assessment |
title_sort |
Electrocatalytic evaluation of DNA damage by superoxide radical for antioxidant capacity assessment |
author |
Barroso, M. Fátima |
author_facet |
Barroso, M. Fátima de-los-Santos-Álvarez, N. Lobo-Castañón, M. J. Miranda-Ordieres, A. J. Delerue-Matos, Cristina Oliveira, M. Beatriz P. P. Tuñón-Blanco, P. |
author_role |
author |
author2 |
de-los-Santos-Álvarez, N. Lobo-Castañón, M. J. Miranda-Ordieres, A. J. Delerue-Matos, Cristina Oliveira, M. Beatriz P. P. Tuñón-Blanco, P. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Politécnico do Porto |
dc.contributor.author.fl_str_mv |
Barroso, M. Fátima de-los-Santos-Álvarez, N. Lobo-Castañón, M. J. Miranda-Ordieres, A. J. Delerue-Matos, Cristina Oliveira, M. Beatriz P. P. Tuñón-Blanco, P. |
dc.subject.por.fl_str_mv |
NADH electrocatalysis DNA damage Antioxidant capacity Ascorbic acid Reactive oxygen species Superoxide radical |
topic |
NADH electrocatalysis DNA damage Antioxidant capacity Ascorbic acid Reactive oxygen species Superoxide radical |
description |
The integrity of DNA purine bases was herein used to evaluate the antioxidant capacity. Unlike other DNA-based antioxidant sensors reported so far, the damaging agent chosen was the O 2 radical enzymatically generated by the xanthine/xanthine oxidase system. An adenine-rich oligonucleotide was adsorbed on carbon paste electrodes and subjected to radical damage in the presence/absence of several antioxidant compounds. As a result, partial damage on DNA was observed. A minor product of the radical oxidation was identified by cyclic voltammetry as a diimine adenine derivative also formed during the electrochemical oxidation of adenine/guanine bases. The protective efficiency of several antioxidant compounds was evaluated after electrochemical oxidation of the remaining unoxidized adenine bases, by measuring the electrocatalytic current of NADH mediated by the adsorbed catalyst species generated. A comparison between O 2 and OH radicals as a source of DNA lesions and the scavenging efficiency of various antioxidant compounds against both of them is discussed. Finally, the antioxidant capacity of beverages was evaluated and compared with the results obtained with an optical method. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011 2011-01-01T00:00:00Z 2013-11-27T17:01:42Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.22/3008 |
url |
http://hdl.handle.net/10400.22/3008 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1572-6657 10.1016/j.jelechem.2011.04.022 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799131334791135232 |