Identification of the first endolysin Cell Binding Domain (CBD) targeting Paenibacillus larvae

Detalhes bibliográficos
Autor(a) principal: Santos, Sílvio B.
Data de Publicação: 2019
Outros Autores: Oliveira, A., Melo, Luís D. R., Azeredo, Joana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/59269
Resumo: Supplementary information accompanies this paper at https://doi.org/10.1038/s41598-019-39097-2.
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spelling Identification of the first endolysin Cell Binding Domain (CBD) targeting Paenibacillus larvaeScience & TechnologySupplementary information accompanies this paper at https://doi.org/10.1038/s41598-019-39097-2.Bacteriophage endolysins present enormous biotechnological potentials and have been successfully used to control and detect bacterial pathogens. Endolysins targeting Gram-positive bacteria are modular, displaying a cell binding (CBD) and an enzymatically active domain. The CBD of phage endolysins are recognized by their high specificity and?host affinity, characteristics that make them promising diagnostic tools. No CBD able to bind Paenibacillus larvae has been identified so far. P. larvae is a Gram-positive spore forming bacteria that causes the American Foulbrood. This highly contagious infection leads to honeybee larvae sepsis and death, resulting in an adverse impact on pollination and on the beekeeping industry. In this work, the first CBD targeting P. larvae was identified and its core binding sequence was investigated. Moreover, it was shown that the domain is highly specific, targeting exclusively P. larvae cells from all ERIC genotypes. The identification of such a domain represents a step forward for the development of effective methods to detect and control this pathogen.This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the project APILYSE,  PTDC/CVT-EPI/4008/2014 (POCI-01-0145-FEDER-016598) and by the strategic funding of UID/BIO/04469/2013 unit, COMPETE 2020 (POCI-01-0145-FEDER-006684) and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte. S.B.S. was supported by  the grant SFRH/BPD/75311/2010 from FCT. We would also like to acknowledge Doctor Vanessa Corby-Harris for gently providing the strain of Parasaccharibacter apium alpha 2.2.info:eu-repo/semantics/publishedVersionNature ResearchUniversidade do MinhoSantos, Sílvio B.Oliveira, A.Melo, Luís D. R.Azeredo, Joana2019-02-222019-02-22T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/59269engSantos, Sílvio B.; Oliveira, A.; Melo, Luís D. R.; Azeredo, Joana, Identification of the first endolysin Cell Binding Domain (CBD) targeting Paenibacillus larvae. Scientific Reports, 9(1), 2568-2568, 20192045-23222045-232210.1038/s41598-019-39097-230796258http://www.nature.com/srep/index.htmlinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:40:26Zoai:repositorium.sdum.uminho.pt:1822/59269Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:37:13.459678Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Identification of the first endolysin Cell Binding Domain (CBD) targeting Paenibacillus larvae
title Identification of the first endolysin Cell Binding Domain (CBD) targeting Paenibacillus larvae
spellingShingle Identification of the first endolysin Cell Binding Domain (CBD) targeting Paenibacillus larvae
Santos, Sílvio B.
Science & Technology
title_short Identification of the first endolysin Cell Binding Domain (CBD) targeting Paenibacillus larvae
title_full Identification of the first endolysin Cell Binding Domain (CBD) targeting Paenibacillus larvae
title_fullStr Identification of the first endolysin Cell Binding Domain (CBD) targeting Paenibacillus larvae
title_full_unstemmed Identification of the first endolysin Cell Binding Domain (CBD) targeting Paenibacillus larvae
title_sort Identification of the first endolysin Cell Binding Domain (CBD) targeting Paenibacillus larvae
author Santos, Sílvio B.
author_facet Santos, Sílvio B.
Oliveira, A.
Melo, Luís D. R.
Azeredo, Joana
author_role author
author2 Oliveira, A.
Melo, Luís D. R.
Azeredo, Joana
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Santos, Sílvio B.
Oliveira, A.
Melo, Luís D. R.
Azeredo, Joana
dc.subject.por.fl_str_mv Science & Technology
topic Science & Technology
description Supplementary information accompanies this paper at https://doi.org/10.1038/s41598-019-39097-2.
publishDate 2019
dc.date.none.fl_str_mv 2019-02-22
2019-02-22T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/59269
url http://hdl.handle.net/1822/59269
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Santos, Sílvio B.; Oliveira, A.; Melo, Luís D. R.; Azeredo, Joana, Identification of the first endolysin Cell Binding Domain (CBD) targeting Paenibacillus larvae. Scientific Reports, 9(1), 2568-2568, 2019
2045-2322
2045-2322
10.1038/s41598-019-39097-2
30796258
http://www.nature.com/srep/index.html
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Nature Research
publisher.none.fl_str_mv Nature Research
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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