Sympatric threatened Iberian leuciscids exhibit differences in Aeromonas diversity and skin lesions' prevalence
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.5/22454 |
Resumo: | Research Areas: Science & Technology - Other Topics |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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7160 |
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Sympatric threatened Iberian leuciscids exhibit differences in Aeromonas diversity and skin lesions' prevalenceHydrophilaIdentificationVeroniiFishGeneAssociationResistanceStrainsCaviaeSPP.Research Areas: Science & Technology - Other TopicsAssessments regarding health aspects of Iberian leuciscids are limited. There is currently an information gap regarding effects of infectious diseases on these populations and their role as a possible conservation threat. Moreover, differences in susceptibility to particular agents, such as Aeromonas spp., by different species/populations is not clear. To understand potential differences in Aeromonas diversity and load, as well as in the prevalence and proportion of skin lesions, in fishes exposed to similar environmental conditions, an observational study was implemented. Using a set of 12 individuals belonging to two sympatric Iberian leuciscid species (Squalius pyrenaicus and Iberochondrostoma lusitanicum), the skin lesion score in each individual was analyzed. Furthermore, a bacterial collection of Aeromonas spp. isolated from each individual was created and isolates’ load was quantified by plate counting, identified at species level using a multiplex-PCR assay and virulence profiles established using classical phenotypic methods. The similarity relationships of the isolates were evaluated using a RAPD analysis. The skin lesion score was significantly higher in S. pyrenaicus, while the Aeromonas spp. load did not differ between species. When analyzing Aeromonas species diversity between fishes, different patterns were observed. A predominance of A. hydrophila was detected in S. pyrenaicus individuals, while I. lusitanicum individuals displayed a more diverse structure. Similarly, the virulence index of isolates from S. pyrenaicus was higher, mostly due to the isolated Aeromonas species. Genomic typing clustered the isolates mainly by fish species and skin lesion score. Specific Aeromonas clusters were associated with higher virulence indexes. Current results suggest potential differences in susceptibility to Aeromonas spp. at the fish species/individual level, and constitute important knowledge for proper wildlife management through the signalization of at-risk fish populations and hierarchization of conservation measures.Abdelazeem Mohamed Algammal, Suez Canal University, EGYPTRepositório da Universidade de LisboaGrilo, MiguelChambel, LéliaMarques, Tiago A.Sousa-Santos, CarlaRobalo, JoanaOliveira, Manuela2021-10-31T23:59:20Z2021-08-052021-08-05T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/22454engGrilo ML, Chambel L, Marques TA, Sousa-Santos C, Robalo JI, Oliveira M. 2021. Sympatric threatened Iberian leuciscids exhibit differences in Aeromonas diversity and skin lesions’ prevalence. PLoS ONE 16(8): e0255850. doi.org/10.1371/journal.pone.02558501932-620310.1371/journal.pone.0255850info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:51:59Zoai:www.repository.utl.pt:10400.5/22454Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:06:52.606211Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Sympatric threatened Iberian leuciscids exhibit differences in Aeromonas diversity and skin lesions' prevalence |
title |
Sympatric threatened Iberian leuciscids exhibit differences in Aeromonas diversity and skin lesions' prevalence |
spellingShingle |
Sympatric threatened Iberian leuciscids exhibit differences in Aeromonas diversity and skin lesions' prevalence Grilo, Miguel Hydrophila Identification Veronii Fish Gene Association Resistance Strains Caviae SPP. |
title_short |
Sympatric threatened Iberian leuciscids exhibit differences in Aeromonas diversity and skin lesions' prevalence |
title_full |
Sympatric threatened Iberian leuciscids exhibit differences in Aeromonas diversity and skin lesions' prevalence |
title_fullStr |
Sympatric threatened Iberian leuciscids exhibit differences in Aeromonas diversity and skin lesions' prevalence |
title_full_unstemmed |
Sympatric threatened Iberian leuciscids exhibit differences in Aeromonas diversity and skin lesions' prevalence |
title_sort |
Sympatric threatened Iberian leuciscids exhibit differences in Aeromonas diversity and skin lesions' prevalence |
author |
Grilo, Miguel |
author_facet |
Grilo, Miguel Chambel, Lélia Marques, Tiago A. Sousa-Santos, Carla Robalo, Joana Oliveira, Manuela |
author_role |
author |
author2 |
Chambel, Lélia Marques, Tiago A. Sousa-Santos, Carla Robalo, Joana Oliveira, Manuela |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
dc.contributor.author.fl_str_mv |
Grilo, Miguel Chambel, Lélia Marques, Tiago A. Sousa-Santos, Carla Robalo, Joana Oliveira, Manuela |
dc.subject.por.fl_str_mv |
Hydrophila Identification Veronii Fish Gene Association Resistance Strains Caviae SPP. |
topic |
Hydrophila Identification Veronii Fish Gene Association Resistance Strains Caviae SPP. |
description |
Research Areas: Science & Technology - Other Topics |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-10-31T23:59:20Z 2021-08-05 2021-08-05T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.5/22454 |
url |
http://hdl.handle.net/10400.5/22454 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Grilo ML, Chambel L, Marques TA, Sousa-Santos C, Robalo JI, Oliveira M. 2021. Sympatric threatened Iberian leuciscids exhibit differences in Aeromonas diversity and skin lesions’ prevalence. PLoS ONE 16(8): e0255850. doi.org/10.1371/journal.pone.0255850 1932-6203 10.1371/journal.pone.0255850 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Abdelazeem Mohamed Algammal, Suez Canal University, EGYPT |
publisher.none.fl_str_mv |
Abdelazeem Mohamed Algammal, Suez Canal University, EGYPT |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799131162004684800 |