Sympatric threatened Iberian leuciscids exhibit differences in Aeromonas diversity and skin lesions' prevalence

Detalhes bibliográficos
Autor(a) principal: Grilo, Miguel
Data de Publicação: 2021
Outros Autores: Chambel, Lélia, Marques, Tiago A., Sousa-Santos, C., Robalo, Joana, Oliveira, Manuela
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/22481
Resumo: Research Areas: Science & Technology - Other Topics
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spelling Sympatric threatened Iberian leuciscids exhibit differences in Aeromonas diversity and skin lesions' prevalenceHydrophilaIdentificationVeroniiFishGeneAssociationResistanceStrainsCaviae spp.Research Areas: Science & Technology - Other TopicsAssessments regarding health aspects of Iberian leuciscids are limited. There is currently an information gap regarding effects of infectious diseases on these populations and their role as a possible conservation threat. Moreover, differences in susceptibility to particular agents, such as Aeromonas spp., by different species/populations is not clear. To understand potential differences in Aeromonas diversity and load, as well as in the prevalence and proportion of skin lesions, in fishes exposed to similar environmental conditions, an observational study was implemented. Using a set of 12 individuals belonging to two sympatric Iberian leuciscid species (Squalius pyrenaicus and Iberochondrostoma lusitanicum), the skin lesion score in each individual was analyzed. Furthermore, a bacterial collection of Aeromonas spp. isolated from each individual was created and isolates’ load was quantified by plate counting, identified at species level using a multiplex-PCR assay and virulence profiles established using classical phenotypic methods. The similarity relationships of the isolates were evaluated using a RAPD analysis. The skin lesion score was significantly higher in S. pyrenaicus, while the Aeromonas spp. load did not differ between species. When analyzing Aeromonas species diversity between fishes, different patterns were observed. A predominance of A. hydrophila was detected in S. pyrenaicus individuals, while I. lusitanicum individuals displayed a more diverse structure. Similarly, the virulence index of isolates from S. pyrenaicus was higher, mostly due to the isolated Aeromonas species. Genomic typing clustered the isolates mainly by fish species and skin lesion score. Specific Aeromonas clusters were associated with higher virulence indexes. Current results suggest potential differences in susceptibility to Aeromonas spp. at the fish species/individual level, and constitute important knowledge for proper wildlife management through the signalization of at-risk fish populations and hierarchization of conservation measures.Public Library ScienceRepositório da Universidade de LisboaGrilo, MiguelChambel, LéliaMarques, Tiago A.Sousa-Santos, C.Robalo, JoanaOliveira, Manuela2021-11-03T16:48:40Z2021-08-052021-08-05T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/22481engGrilo ML, Chambel L, Marques TA, Sousa-Santos C, Robalo JI, Oliveira M. 2021. Sympatric threatened Iberian leuciscids exhibit differences in Aeromonas diversity and skin lesions’ prevalence. PLoS ONE 16(8):e0255850. DOI: 10.1371/journal.pone.02558501932-620310.1371/journal.pone.0255850info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:52:00Zoai:www.repository.utl.pt:10400.5/22481Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:06:53.880737Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Sympatric threatened Iberian leuciscids exhibit differences in Aeromonas diversity and skin lesions' prevalence
title Sympatric threatened Iberian leuciscids exhibit differences in Aeromonas diversity and skin lesions' prevalence
spellingShingle Sympatric threatened Iberian leuciscids exhibit differences in Aeromonas diversity and skin lesions' prevalence
Grilo, Miguel
Hydrophila
Identification
Veronii
Fish
Gene
Association
Resistance
Strains
Caviae spp.
title_short Sympatric threatened Iberian leuciscids exhibit differences in Aeromonas diversity and skin lesions' prevalence
title_full Sympatric threatened Iberian leuciscids exhibit differences in Aeromonas diversity and skin lesions' prevalence
title_fullStr Sympatric threatened Iberian leuciscids exhibit differences in Aeromonas diversity and skin lesions' prevalence
title_full_unstemmed Sympatric threatened Iberian leuciscids exhibit differences in Aeromonas diversity and skin lesions' prevalence
title_sort Sympatric threatened Iberian leuciscids exhibit differences in Aeromonas diversity and skin lesions' prevalence
author Grilo, Miguel
author_facet Grilo, Miguel
Chambel, Lélia
Marques, Tiago A.
Sousa-Santos, C.
Robalo, Joana
Oliveira, Manuela
author_role author
author2 Chambel, Lélia
Marques, Tiago A.
Sousa-Santos, C.
Robalo, Joana
Oliveira, Manuela
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Grilo, Miguel
Chambel, Lélia
Marques, Tiago A.
Sousa-Santos, C.
Robalo, Joana
Oliveira, Manuela
dc.subject.por.fl_str_mv Hydrophila
Identification
Veronii
Fish
Gene
Association
Resistance
Strains
Caviae spp.
topic Hydrophila
Identification
Veronii
Fish
Gene
Association
Resistance
Strains
Caviae spp.
description Research Areas: Science & Technology - Other Topics
publishDate 2021
dc.date.none.fl_str_mv 2021-11-03T16:48:40Z
2021-08-05
2021-08-05T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/22481
url http://hdl.handle.net/10400.5/22481
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Grilo ML, Chambel L, Marques TA, Sousa-Santos C, Robalo JI, Oliveira M. 2021. Sympatric threatened Iberian leuciscids exhibit differences in Aeromonas diversity and skin lesions’ prevalence. PLoS ONE 16(8):e0255850. DOI: 10.1371/journal.pone.0255850
1932-6203
10.1371/journal.pone.0255850
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Public Library Science
publisher.none.fl_str_mv Public Library Science
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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