Collagen Type III as a Possible Blood Biomarker of Fibrosis in Equine Endometrium

Detalhes bibliográficos
Autor(a) principal: Alpoim-Moreira, Joana
Data de Publicação: 2022
Outros Autores: Fernandes, Carina, Rebordão, Maria Rosa, Costa, Ana Luísa, BliebernichT, Miguel, Nunes, Telmo, Szóstek-Mioduchowska, Anna, Skarzynski, Dariusz J., Ferreira-Dias, Graça
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/26963
Resumo: Research Areas:Agriculture ; Veterinary Sciences ; Zoology
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spelling Collagen Type III as a Possible Blood Biomarker of Fibrosis in Equine EndometriumCollagenBiomarkerBloodEndometriumFibrosisMareEndometriosisResearch Areas:Agriculture ; Veterinary Sciences ; ZoologyCollagen pathological deposition in equine endometrium (endometrosis) is responsible for infertility. Kenney and Doig’s endometrial biopsy histopathological classification is the gold standard method for endometrosis evaluation, whereby blood biomarkers identification would be less invasive and could provide additional information regarding endometrosis diagnosis and fertility prognosis. This study aimed to identify blood biomarkers for endometrosis diagnosis (42 mares were used in experiment 1), and fertility assessment (50 mares were used in experiment 2). Reproductive examination, endometrial biopsy histopathological classification (Kenney and Doig) and blood collection were performed. Endometrium and serum collagen type I (COL1) and type III (COL3), and hydroxyproline concentrations were measured (ELISA). Serum COL3 cut-off value of 60.9 ng/mL allowed healthy endometria (category I) differentiation from endometria with degenerative/fibrotic lesions (categories IIA, IIB or III) with 100% specificity and 75.9% sensitivity. This cut-off value enabled category I + IIA differentiation from IIB + III (76% specificity, 81% sensitivity), and category III differentiation from others (65% specificity, 92.3% sensitivity). COL1 and hydroxyproline were not valid as blood biomarkers. Serum COL3 cut-off value of 146 ng/mL differentiated fertile from infertile mares (82.4% specificity, 55.6% sensitivity), and was not correlated with mares’ age. Only COL3 may prove useful as a diagnostic aid in mares with endometrial fibrosis and as a fertility indicator.MDPIRepositório da Universidade de LisboaAlpoim-Moreira, JoanaFernandes, CarinaRebordão, Maria RosaCosta, Ana LuísaBliebernichT, MiguelNunes, TelmoSzóstek-Mioduchowska, AnnaSkarzynski, Dariusz J.Ferreira-Dias, Graça2023-01-19T16:54:11Z2022-07-212022-07-21T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/26963engAlpoim-Moreira J, Fernandes C, Rebordão MR, Costa AL, Bliebernicht M, Nunes T, Szóstek-Mioduchowska A, Skarzynski DJ, Ferreira-Dias G. 2022. Collagen Type III as a Possible Blood Biomarker of Fibrosis in Equine Endometrium. Animals, 12:1854 DOI:https://doi.org/10.3390/ ani121418542076-261510.3390/ ani12141854info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:56:23Zoai:www.repository.utl.pt:10400.5/26963Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:10:30.554966Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Collagen Type III as a Possible Blood Biomarker of Fibrosis in Equine Endometrium
title Collagen Type III as a Possible Blood Biomarker of Fibrosis in Equine Endometrium
spellingShingle Collagen Type III as a Possible Blood Biomarker of Fibrosis in Equine Endometrium
Alpoim-Moreira, Joana
Collagen
Biomarker
Blood
Endometrium
Fibrosis
Mare
Endometriosis
title_short Collagen Type III as a Possible Blood Biomarker of Fibrosis in Equine Endometrium
title_full Collagen Type III as a Possible Blood Biomarker of Fibrosis in Equine Endometrium
title_fullStr Collagen Type III as a Possible Blood Biomarker of Fibrosis in Equine Endometrium
title_full_unstemmed Collagen Type III as a Possible Blood Biomarker of Fibrosis in Equine Endometrium
title_sort Collagen Type III as a Possible Blood Biomarker of Fibrosis in Equine Endometrium
author Alpoim-Moreira, Joana
author_facet Alpoim-Moreira, Joana
Fernandes, Carina
Rebordão, Maria Rosa
Costa, Ana Luísa
BliebernichT, Miguel
Nunes, Telmo
Szóstek-Mioduchowska, Anna
Skarzynski, Dariusz J.
Ferreira-Dias, Graça
author_role author
author2 Fernandes, Carina
Rebordão, Maria Rosa
Costa, Ana Luísa
BliebernichT, Miguel
Nunes, Telmo
Szóstek-Mioduchowska, Anna
Skarzynski, Dariusz J.
Ferreira-Dias, Graça
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Alpoim-Moreira, Joana
Fernandes, Carina
Rebordão, Maria Rosa
Costa, Ana Luísa
BliebernichT, Miguel
Nunes, Telmo
Szóstek-Mioduchowska, Anna
Skarzynski, Dariusz J.
Ferreira-Dias, Graça
dc.subject.por.fl_str_mv Collagen
Biomarker
Blood
Endometrium
Fibrosis
Mare
Endometriosis
topic Collagen
Biomarker
Blood
Endometrium
Fibrosis
Mare
Endometriosis
description Research Areas:Agriculture ; Veterinary Sciences ; Zoology
publishDate 2022
dc.date.none.fl_str_mv 2022-07-21
2022-07-21T00:00:00Z
2023-01-19T16:54:11Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/26963
url http://hdl.handle.net/10400.5/26963
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Alpoim-Moreira J, Fernandes C, Rebordão MR, Costa AL, Bliebernicht M, Nunes T, Szóstek-Mioduchowska A, Skarzynski DJ, Ferreira-Dias G. 2022. Collagen Type III as a Possible Blood Biomarker of Fibrosis in Equine Endometrium. Animals, 12:1854 DOI:https://doi.org/10.3390/ ani12141854
2076-2615
10.3390/ ani12141854
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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