Zearalenone and its derivatives α-Zearalenol and β-Zearalenol decontamination by Saccharomyces cerevisiae strains isolated from bovine forage

• Autor(a) principal: Keller, Luiz
• Data de Publicação: 2015
• Outros Autores: Abrunhosa, Luís, Keller, Kelly, Rosa, Carlos Alberto, Cavaglieri, Lilia, Venâncio, Armando
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/1822/36841
• topic: ZEA; α-ZOL; β-ZOL; Detoxification; Adsorption; Saccharomyces cerevisiae; ß-ZOL; Ciências Naturais::Ciências Biológicas; Ciências Agrárias::Biotecnologia Agrária e Alimentar; Science & Technology

Zearalenone (ZEA) and its derivatives are mycotoxins with estrogenic effects on mammals. The biotransformation for ZEA in animals involves the formation of two major metabolites, - and -zearalenol (-ZOL and -ZOL), which are subsequently conjugated with glucuronic acid. The capability of Saccharomyces cerevisiae strains isolated from silage to eliminate ZEA and its derivatives -ZOL and -ZOL was investigated as, also, the mechanisms involved. Strains were grown on Yeast Extract-Peptone-Dextrose medium supplemented with the mycotoxins and their elimination from medium was quantified over time by HPLC-FL. A significant effect on the concentration of ZEA was observed, as all the tested strains were able to eliminate more than 90% of the mycotoxin from the culture medium in two days. The observed elimination was mainly due to ZEA biotransformation into -ZOL (53%) and -ZOL (8%) rather than to its adsorption to yeast cells walls. Further, the biotransformation of -ZOL was not observed but a small amount of -ZOL (6%) disappeared from culture medium. ZEA biotransformation by yeasts may not be regarded as a full detoxification process because both main end-products are still estrogenic. Nonetheless, it was observed that the biotransformation favors the formation of -ZOL which is less estrogenic than ZEA and -ZOL. This metabolic effect is only possible if active strains are used as feed additives and may play a role in the detoxification performance of products with viable S. cerevisiae cells.