Zearalenone and its derivatives α-Zearalenol and β-Zearalenol decontamination by Saccharomyces cerevisiae strains isolated from bovine forage
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/36841 |
Resumo: | Zearalenone (ZEA) and its derivatives are mycotoxins with estrogenic effects on mammals. The biotransformation for ZEA in animals involves the formation of two major metabolites, - and -zearalenol (-ZOL and -ZOL), which are subsequently conjugated with glucuronic acid. The capability of Saccharomyces cerevisiae strains isolated from silage to eliminate ZEA and its derivatives -ZOL and -ZOL was investigated as, also, the mechanisms involved. Strains were grown on Yeast Extract-Peptone-Dextrose medium supplemented with the mycotoxins and their elimination from medium was quantified over time by HPLC-FL. A significant effect on the concentration of ZEA was observed, as all the tested strains were able to eliminate more than 90% of the mycotoxin from the culture medium in two days. The observed elimination was mainly due to ZEA biotransformation into -ZOL (53%) and -ZOL (8%) rather than to its adsorption to yeast cells walls. Further, the biotransformation of -ZOL was not observed but a small amount of -ZOL (6%) disappeared from culture medium. ZEA biotransformation by yeasts may not be regarded as a full detoxification process because both main end-products are still estrogenic. Nonetheless, it was observed that the biotransformation favors the formation of -ZOL which is less estrogenic than ZEA and -ZOL. This metabolic effect is only possible if active strains are used as feed additives and may play a role in the detoxification performance of products with viable S. cerevisiae cells. |
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Zearalenone and its derivatives α-Zearalenol and β-Zearalenol decontamination by Saccharomyces cerevisiae strains isolated from bovine forageZEAα-ZOLβ-ZOLDetoxificationAdsorptionSaccharomyces cerevisiaeß-ZOLCiências Naturais::Ciências BiológicasCiências Agrárias::Biotecnologia Agrária e AlimentarScience & TechnologyZearalenone (ZEA) and its derivatives are mycotoxins with estrogenic effects on mammals. The biotransformation for ZEA in animals involves the formation of two major metabolites, - and -zearalenol (-ZOL and -ZOL), which are subsequently conjugated with glucuronic acid. The capability of Saccharomyces cerevisiae strains isolated from silage to eliminate ZEA and its derivatives -ZOL and -ZOL was investigated as, also, the mechanisms involved. Strains were grown on Yeast Extract-Peptone-Dextrose medium supplemented with the mycotoxins and their elimination from medium was quantified over time by HPLC-FL. A significant effect on the concentration of ZEA was observed, as all the tested strains were able to eliminate more than 90% of the mycotoxin from the culture medium in two days. The observed elimination was mainly due to ZEA biotransformation into -ZOL (53%) and -ZOL (8%) rather than to its adsorption to yeast cells walls. Further, the biotransformation of -ZOL was not observed but a small amount of -ZOL (6%) disappeared from culture medium. ZEA biotransformation by yeasts may not be regarded as a full detoxification process because both main end-products are still estrogenic. Nonetheless, it was observed that the biotransformation favors the formation of -ZOL which is less estrogenic than ZEA and -ZOL. This metabolic effect is only possible if active strains are used as feed additives and may play a role in the detoxification performance of products with viable S. cerevisiae cells.Grants from CYTED, CNPq, CAPES-DS, and FAPUR/UFRRJ (Brazil). This work was also funded by FEDER funds through the “Programa Operacional Factores de Competitividade-COMPETE” and by national funds through “Fundação para a Ciência e a Tecnologia -FCT”, Ref. FCOMP-01-0124-F EDER-028029 and PTDC/AGR-TEC/3900/2012,respectively. FCT Strategic Project PEst-OE/EQB/LA0023/2013 and the Project “BioInd - Biotechnology and Bioengineering for improved Indus trial and Agro-Food processes, REF. NORTE-07-0124-FEDER-000028” Co-funded by the Programa Operacional Regional do Norte (ON.2-O Novo Norte), QREN, FEDER. Grant Incentivo/EQB/LA0023/2014 from ON.2-O Novo Norte.MDPI PublishingUniversidade do MinhoKeller, LuizAbrunhosa, LuísKeller, KellyRosa, Carlos AlbertoCavaglieri, LiliaVenâncio, Armando2015-082015-08-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/36841engKeller, Luiz; Abrunhosa, Luís; Keller, Kelly; Rosa, Carlos Alberto; Cavaglieri, Lilia; Venâncio, Armando, Zearalenone and its derivatives α-Zearalenol and β-zearalenol decontamination by Saccharomyces cerevisiae strains isolated from bovine forage. Toxins, 7(8), 3297-3308, 20152072-66512072-665110.3390/toxins708329726308051http://www.mdpi.com/2072-6651/7/8/3297info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:34:30Zoai:repositorium.sdum.uminho.pt:1822/36841Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:30:11.902997Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Zearalenone and its derivatives α-Zearalenol and β-Zearalenol decontamination by Saccharomyces cerevisiae strains isolated from bovine forage |
title |
Zearalenone and its derivatives α-Zearalenol and β-Zearalenol decontamination by Saccharomyces cerevisiae strains isolated from bovine forage |
spellingShingle |
Zearalenone and its derivatives α-Zearalenol and β-Zearalenol decontamination by Saccharomyces cerevisiae strains isolated from bovine forage Keller, Luiz ZEA α-ZOL β-ZOL Detoxification Adsorption Saccharomyces cerevisiae ß-ZOL Ciências Naturais::Ciências Biológicas Ciências Agrárias::Biotecnologia Agrária e Alimentar Science & Technology |
title_short |
Zearalenone and its derivatives α-Zearalenol and β-Zearalenol decontamination by Saccharomyces cerevisiae strains isolated from bovine forage |
title_full |
Zearalenone and its derivatives α-Zearalenol and β-Zearalenol decontamination by Saccharomyces cerevisiae strains isolated from bovine forage |
title_fullStr |
Zearalenone and its derivatives α-Zearalenol and β-Zearalenol decontamination by Saccharomyces cerevisiae strains isolated from bovine forage |
title_full_unstemmed |
Zearalenone and its derivatives α-Zearalenol and β-Zearalenol decontamination by Saccharomyces cerevisiae strains isolated from bovine forage |
title_sort |
Zearalenone and its derivatives α-Zearalenol and β-Zearalenol decontamination by Saccharomyces cerevisiae strains isolated from bovine forage |
author |
Keller, Luiz |
author_facet |
Keller, Luiz Abrunhosa, Luís Keller, Kelly Rosa, Carlos Alberto Cavaglieri, Lilia Venâncio, Armando |
author_role |
author |
author2 |
Abrunhosa, Luís Keller, Kelly Rosa, Carlos Alberto Cavaglieri, Lilia Venâncio, Armando |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Keller, Luiz Abrunhosa, Luís Keller, Kelly Rosa, Carlos Alberto Cavaglieri, Lilia Venâncio, Armando |
dc.subject.por.fl_str_mv |
ZEA α-ZOL β-ZOL Detoxification Adsorption Saccharomyces cerevisiae ß-ZOL Ciências Naturais::Ciências Biológicas Ciências Agrárias::Biotecnologia Agrária e Alimentar Science & Technology |
topic |
ZEA α-ZOL β-ZOL Detoxification Adsorption Saccharomyces cerevisiae ß-ZOL Ciências Naturais::Ciências Biológicas Ciências Agrárias::Biotecnologia Agrária e Alimentar Science & Technology |
description |
Zearalenone (ZEA) and its derivatives are mycotoxins with estrogenic effects on mammals. The biotransformation for ZEA in animals involves the formation of two major metabolites, - and -zearalenol (-ZOL and -ZOL), which are subsequently conjugated with glucuronic acid. The capability of Saccharomyces cerevisiae strains isolated from silage to eliminate ZEA and its derivatives -ZOL and -ZOL was investigated as, also, the mechanisms involved. Strains were grown on Yeast Extract-Peptone-Dextrose medium supplemented with the mycotoxins and their elimination from medium was quantified over time by HPLC-FL. A significant effect on the concentration of ZEA was observed, as all the tested strains were able to eliminate more than 90% of the mycotoxin from the culture medium in two days. The observed elimination was mainly due to ZEA biotransformation into -ZOL (53%) and -ZOL (8%) rather than to its adsorption to yeast cells walls. Further, the biotransformation of -ZOL was not observed but a small amount of -ZOL (6%) disappeared from culture medium. ZEA biotransformation by yeasts may not be regarded as a full detoxification process because both main end-products are still estrogenic. Nonetheless, it was observed that the biotransformation favors the formation of -ZOL which is less estrogenic than ZEA and -ZOL. This metabolic effect is only possible if active strains are used as feed additives and may play a role in the detoxification performance of products with viable S. cerevisiae cells. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-08 2015-08-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/36841 |
url |
http://hdl.handle.net/1822/36841 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Keller, Luiz; Abrunhosa, Luís; Keller, Kelly; Rosa, Carlos Alberto; Cavaglieri, Lilia; Venâncio, Armando, Zearalenone and its derivatives α-Zearalenol and β-zearalenol decontamination by Saccharomyces cerevisiae strains isolated from bovine forage. Toxins, 7(8), 3297-3308, 2015 2072-6651 2072-6651 10.3390/toxins7083297 26308051 http://www.mdpi.com/2072-6651/7/8/3297 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI Publishing |
publisher.none.fl_str_mv |
MDPI Publishing |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132804928241664 |