Multilocus sequence identification of penicillium species in cork bark during plank preparation for the manufacture of stoppers
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/7831 |
Resumo: | Despite several studies reporting Penicillium as one of the most frequent fungal genera in cork planks, the isolates were rarely identified to species level.We conducted a detailed study to identify Penicillium species from the field to the factory environment prior to and after boiling the cork planks. A total of 84 samples were analyzed. Of the 486 Penicillium isolates phenotypically identified, 32 representative or unusual strains were selected for identification by multilocus DNA sequence type. Cork proved to be a rich source of Penicillium biodiversity. A total of 30 taxa were recognized from cork including rarely seen species and 6 phylogenetically unique groups. Spores of some species lodged deep in cork can survive the boiling process. P. glabrum, P. glandicola and P. toxicarium, species with high CFU numbers in the field, are still frequently present in cork after boiling. Other species are killed by the boiling treatment and replaced by Penicillium species originating from the factory environment. Species known to contribute to cork taint were isolated at all stages. Good manufacturing practices are necessary at all stages in the preparation of cork planks to minimize the load of Penicillium species that produce cork taint. |
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Multilocus sequence identification of penicillium species in cork bark during plank preparation for the manufacture of stoppersPenicilliumMycologyIndustrial microbiologyCorkBiodiversityTaxonomyScience & TechnologyDespite several studies reporting Penicillium as one of the most frequent fungal genera in cork planks, the isolates were rarely identified to species level.We conducted a detailed study to identify Penicillium species from the field to the factory environment prior to and after boiling the cork planks. A total of 84 samples were analyzed. Of the 486 Penicillium isolates phenotypically identified, 32 representative or unusual strains were selected for identification by multilocus DNA sequence type. Cork proved to be a rich source of Penicillium biodiversity. A total of 30 taxa were recognized from cork including rarely seen species and 6 phylogenetically unique groups. Spores of some species lodged deep in cork can survive the boiling process. P. glabrum, P. glandicola and P. toxicarium, species with high CFU numbers in the field, are still frequently present in cork after boiling. Other species are killed by the boiling treatment and replaced by Penicillium species originating from the factory environment. Species known to contribute to cork taint were isolated at all stages. Good manufacturing practices are necessary at all stages in the preparation of cork planks to minimize the load of Penicillium species that produce cork taint.CTCOR.Fundação para a Ciência e a Tecnologia (FCT)ElsevierUniversidade do MinhoSerra, RitaPeterson, Stephen W.Centro Tecnológico da CortiçaVenâncio, Armando2008-042008-04-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/7831eng"Research in Microbiology". ISSN 0923-2508. 159:3 (Apr. 2008) 178-186.0923-250810.1016/j.resmic.2007.12.00918321681info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:23:07Zoai:repositorium.sdum.uminho.pt:1822/7831Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:16:45.933404Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Multilocus sequence identification of penicillium species in cork bark during plank preparation for the manufacture of stoppers |
title |
Multilocus sequence identification of penicillium species in cork bark during plank preparation for the manufacture of stoppers |
spellingShingle |
Multilocus sequence identification of penicillium species in cork bark during plank preparation for the manufacture of stoppers Serra, Rita Penicillium Mycology Industrial microbiology Cork Biodiversity Taxonomy Science & Technology |
title_short |
Multilocus sequence identification of penicillium species in cork bark during plank preparation for the manufacture of stoppers |
title_full |
Multilocus sequence identification of penicillium species in cork bark during plank preparation for the manufacture of stoppers |
title_fullStr |
Multilocus sequence identification of penicillium species in cork bark during plank preparation for the manufacture of stoppers |
title_full_unstemmed |
Multilocus sequence identification of penicillium species in cork bark during plank preparation for the manufacture of stoppers |
title_sort |
Multilocus sequence identification of penicillium species in cork bark during plank preparation for the manufacture of stoppers |
author |
Serra, Rita |
author_facet |
Serra, Rita Peterson, Stephen W. Centro Tecnológico da Cortiça Venâncio, Armando |
author_role |
author |
author2 |
Peterson, Stephen W. Centro Tecnológico da Cortiça Venâncio, Armando |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Serra, Rita Peterson, Stephen W. Centro Tecnológico da Cortiça Venâncio, Armando |
dc.subject.por.fl_str_mv |
Penicillium Mycology Industrial microbiology Cork Biodiversity Taxonomy Science & Technology |
topic |
Penicillium Mycology Industrial microbiology Cork Biodiversity Taxonomy Science & Technology |
description |
Despite several studies reporting Penicillium as one of the most frequent fungal genera in cork planks, the isolates were rarely identified to species level.We conducted a detailed study to identify Penicillium species from the field to the factory environment prior to and after boiling the cork planks. A total of 84 samples were analyzed. Of the 486 Penicillium isolates phenotypically identified, 32 representative or unusual strains were selected for identification by multilocus DNA sequence type. Cork proved to be a rich source of Penicillium biodiversity. A total of 30 taxa were recognized from cork including rarely seen species and 6 phylogenetically unique groups. Spores of some species lodged deep in cork can survive the boiling process. P. glabrum, P. glandicola and P. toxicarium, species with high CFU numbers in the field, are still frequently present in cork after boiling. Other species are killed by the boiling treatment and replaced by Penicillium species originating from the factory environment. Species known to contribute to cork taint were isolated at all stages. Good manufacturing practices are necessary at all stages in the preparation of cork planks to minimize the load of Penicillium species that produce cork taint. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-04 2008-04-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/7831 |
url |
http://hdl.handle.net/1822/7831 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
"Research in Microbiology". ISSN 0923-2508. 159:3 (Apr. 2008) 178-186. 0923-2508 10.1016/j.resmic.2007.12.009 18321681 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799132618157981696 |