Comparative biochemical profile of protein-rich products obtained from industrial rapeseed meal
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.14/23727 |
Resumo: | Protein-rich products, prepared from industrial rapeseed meal, have the potential for versatile applications in the food-, feed-, and nutraceutical industries. The aim of study was to characterize the biochemical composition of two protein-rich products obtained from industrially produced rapeseed meal. Protein isolate (PI) and acid soluble protein (ASP) were prepared by alkaline extraction (pH 12.0) followed by isoelectric precipitation (pH 4.5). Biochemical analyses revealed that PI contained a high protein amount (86.9%), while ASP had 28.8% protein and a relatively high level of non- protein compounds including ash (20.6%) and fiber (30.0%). Results showed that neither protein products contained glucosinolates. They were rich in microelements; the most abundant were Cu (64.3 mg kg-1) and Fe (133 mg kg-1) in PI and Mn (39.7 mg kg-1) and Zn (84.2 mg kg-1) in ASP. PI and ASP were also a good source of Se (1.1 mg kg-1 and 0.9 mg kg-1, respectively). Lysine was the most abundant essential amino acid in PI with amino acid score of 100.7% followed by leucine (98.3%) and valine (95.8%). Both protein products were mainly composed of low molecular weight fractions (5 to 33 kDa) but in different ratio. The PI contained two fractions with molecular weights 53 and 235 kDa which were not found in ASP. In conclusion, PI and ASP exhibited different biochemical characteristics which make them suitable for different applications. |
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Comparative biochemical profile of protein-rich products obtained from industrial rapeseed mealAcid soluble proteinIndustrial rapeseed mealProtein isolateProtein-rich products, prepared from industrial rapeseed meal, have the potential for versatile applications in the food-, feed-, and nutraceutical industries. The aim of study was to characterize the biochemical composition of two protein-rich products obtained from industrially produced rapeseed meal. Protein isolate (PI) and acid soluble protein (ASP) were prepared by alkaline extraction (pH 12.0) followed by isoelectric precipitation (pH 4.5). Biochemical analyses revealed that PI contained a high protein amount (86.9%), while ASP had 28.8% protein and a relatively high level of non- protein compounds including ash (20.6%) and fiber (30.0%). Results showed that neither protein products contained glucosinolates. They were rich in microelements; the most abundant were Cu (64.3 mg kg-1) and Fe (133 mg kg-1) in PI and Mn (39.7 mg kg-1) and Zn (84.2 mg kg-1) in ASP. PI and ASP were also a good source of Se (1.1 mg kg-1 and 0.9 mg kg-1, respectively). Lysine was the most abundant essential amino acid in PI with amino acid score of 100.7% followed by leucine (98.3%) and valine (95.8%). Both protein products were mainly composed of low molecular weight fractions (5 to 33 kDa) but in different ratio. The PI contained two fractions with molecular weights 53 and 235 kDa which were not found in ASP. In conclusion, PI and ASP exhibited different biochemical characteristics which make them suitable for different applications.United Arab Emirates UniversityVeritati - Repositório Institucional da Universidade Católica PortuguesaIvanova, PetyaKalaydzhiev, HristoRustad, TuridSilva, Cristina L. M.Chalova, Vesela I.2017-12-18T19:32:53Z20172017-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/23727engIvanova, P., Kalaydzhiev, H., Rustad, T., Silva, C. L. M., & Chalova, V. I. (2017). Comparative biochemical profile of protein-rich products obtained from industrial rapeseed meal. Emirates Journal of Food and Agriculture, 29(3), 170-1782079-052X10.9755/ejfa.2016-11-17602079-053885013409469000396593900002info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-09-12T01:38:19Zoai:repositorio.ucp.pt:10400.14/23727Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:19:29.204801Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Comparative biochemical profile of protein-rich products obtained from industrial rapeseed meal |
title |
Comparative biochemical profile of protein-rich products obtained from industrial rapeseed meal |
spellingShingle |
Comparative biochemical profile of protein-rich products obtained from industrial rapeseed meal Ivanova, Petya Acid soluble protein Industrial rapeseed meal Protein isolate |
title_short |
Comparative biochemical profile of protein-rich products obtained from industrial rapeseed meal |
title_full |
Comparative biochemical profile of protein-rich products obtained from industrial rapeseed meal |
title_fullStr |
Comparative biochemical profile of protein-rich products obtained from industrial rapeseed meal |
title_full_unstemmed |
Comparative biochemical profile of protein-rich products obtained from industrial rapeseed meal |
title_sort |
Comparative biochemical profile of protein-rich products obtained from industrial rapeseed meal |
author |
Ivanova, Petya |
author_facet |
Ivanova, Petya Kalaydzhiev, Hristo Rustad, Turid Silva, Cristina L. M. Chalova, Vesela I. |
author_role |
author |
author2 |
Kalaydzhiev, Hristo Rustad, Turid Silva, Cristina L. M. Chalova, Vesela I. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Veritati - Repositório Institucional da Universidade Católica Portuguesa |
dc.contributor.author.fl_str_mv |
Ivanova, Petya Kalaydzhiev, Hristo Rustad, Turid Silva, Cristina L. M. Chalova, Vesela I. |
dc.subject.por.fl_str_mv |
Acid soluble protein Industrial rapeseed meal Protein isolate |
topic |
Acid soluble protein Industrial rapeseed meal Protein isolate |
description |
Protein-rich products, prepared from industrial rapeseed meal, have the potential for versatile applications in the food-, feed-, and nutraceutical industries. The aim of study was to characterize the biochemical composition of two protein-rich products obtained from industrially produced rapeseed meal. Protein isolate (PI) and acid soluble protein (ASP) were prepared by alkaline extraction (pH 12.0) followed by isoelectric precipitation (pH 4.5). Biochemical analyses revealed that PI contained a high protein amount (86.9%), while ASP had 28.8% protein and a relatively high level of non- protein compounds including ash (20.6%) and fiber (30.0%). Results showed that neither protein products contained glucosinolates. They were rich in microelements; the most abundant were Cu (64.3 mg kg-1) and Fe (133 mg kg-1) in PI and Mn (39.7 mg kg-1) and Zn (84.2 mg kg-1) in ASP. PI and ASP were also a good source of Se (1.1 mg kg-1 and 0.9 mg kg-1, respectively). Lysine was the most abundant essential amino acid in PI with amino acid score of 100.7% followed by leucine (98.3%) and valine (95.8%). Both protein products were mainly composed of low molecular weight fractions (5 to 33 kDa) but in different ratio. The PI contained two fractions with molecular weights 53 and 235 kDa which were not found in ASP. In conclusion, PI and ASP exhibited different biochemical characteristics which make them suitable for different applications. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-12-18T19:32:53Z 2017 2017-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.14/23727 |
url |
http://hdl.handle.net/10400.14/23727 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Ivanova, P., Kalaydzhiev, H., Rustad, T., Silva, C. L. M., & Chalova, V. I. (2017). Comparative biochemical profile of protein-rich products obtained from industrial rapeseed meal. Emirates Journal of Food and Agriculture, 29(3), 170-178 2079-052X 10.9755/ejfa.2016-11-1760 2079-0538 85013409469 000396593900002 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
United Arab Emirates University |
publisher.none.fl_str_mv |
United Arab Emirates University |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799131889801363456 |