Fabrication of a novel hierarchical fibrous scaffold for breast cancer cell culture

Detalhes bibliográficos
Autor(a) principal: Luo, Honglin
Data de Publicação: 2019
Outros Autores: Cui, Teng, Gan, Deqiang, Gama, F. M., Zhang, Quanchao, Wan, Yizao
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/65839
Resumo: Supplementary data to this article can be found online at https://doi.org/10.1016/j.polymertesting.2019.106107.
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spelling Fabrication of a novel hierarchical fibrous scaffold for breast cancer cell cultureCellulose fiberHierarchical structureCancer cellcultureIn vitro cultureScience & TechnologySupplementary data to this article can be found online at https://doi.org/10.1016/j.polymertesting.2019.106107.Scaffolds combining nano- and submicro-fibers closely mimicking extracellular matrix (ECM) have been poorly exploited for in vitro cancer cell culture. Herein, a combined electrospinning and modified in situ biosynthesis method has been developed to fabricate a novel scaffold consisting of bacterial cellulose (BC) nanofibers and electrospun cellulose acetate (CA) submicrofibers to mimic the fibrillar structure of natural ECM. The CA/BC nano/submicrofibrous scaffold was characterized by scanning electron microscopy (SEM), mechanical strength tests, porosity measurements, and cell studies using the MCF-7 breast cancer cells. In addition, the sensitivity of the cancer cells seeded in the CA/BC nano/submicrofibrous scaffold to an anticancer drug was assessed. It was found that the CA/BC scaffold exhibited an interconnected porous structure in which BC nanofibers penetrated into the submicrofibrous CA scaffold. Such sophisticated structure was responsible for the improved mechanical properties of CA/BC scaffold over the ones obtained using a single kind of fibers. More importantly, the CA/BC scaffold showed improved cell adhesion, migration, and proliferation over single BC or CA scaffold. Finally, cells grown on CA/BC scaffold exhibited a greater doxorubicin resistance than those on single CA or BC scaffold. The results suggest that the CA/BC nano/submicrofibrous scaffold has potential for application in in vitro tumor model for the study of cancer progression and drug screening.This work was supported by the Key Project of Natural Science Foundation of Jiangxi Province (Grant no. 20161ACB20018), the National Natural Science Foundation of China (Grant nos. 31870963, 31660264, and 51572187), the Youth Science Foundation of Jiangxi Province (Grant no. 20181BAB216010), and the Science and Technology Research Project of Jiangxi Education Department (Grant no. GJJ180348).info:eu-repo/semantics/publishedVersionElsevierUniversidade do MinhoLuo, HonglinCui, TengGan, DeqiangGama, F. M.Zhang, QuanchaoWan, Yizao20192019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/65839engLuo, Honglin; Cui, Teng; Gan, Deqiang; Gama, F. M.; Zhang, Quanchao; Wan, Yizao, Fabrication of a novel hierarchical fibrous scaffold for breast cancer cell culture. Polymer Testing, 80(106107), 20190142-941810.1016/j.polymertesting.2019.106107http://www.journals.elsevier.com/polymer-testing/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:11:17Zoai:repositorium.sdum.uminho.pt:1822/65839Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:03:03.212065Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Fabrication of a novel hierarchical fibrous scaffold for breast cancer cell culture
title Fabrication of a novel hierarchical fibrous scaffold for breast cancer cell culture
spellingShingle Fabrication of a novel hierarchical fibrous scaffold for breast cancer cell culture
Luo, Honglin
Cellulose fiber
Hierarchical structure
Cancer cell
culture
In vitro culture
Science & Technology
title_short Fabrication of a novel hierarchical fibrous scaffold for breast cancer cell culture
title_full Fabrication of a novel hierarchical fibrous scaffold for breast cancer cell culture
title_fullStr Fabrication of a novel hierarchical fibrous scaffold for breast cancer cell culture
title_full_unstemmed Fabrication of a novel hierarchical fibrous scaffold for breast cancer cell culture
title_sort Fabrication of a novel hierarchical fibrous scaffold for breast cancer cell culture
author Luo, Honglin
author_facet Luo, Honglin
Cui, Teng
Gan, Deqiang
Gama, F. M.
Zhang, Quanchao
Wan, Yizao
author_role author
author2 Cui, Teng
Gan, Deqiang
Gama, F. M.
Zhang, Quanchao
Wan, Yizao
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Luo, Honglin
Cui, Teng
Gan, Deqiang
Gama, F. M.
Zhang, Quanchao
Wan, Yizao
dc.subject.por.fl_str_mv Cellulose fiber
Hierarchical structure
Cancer cell
culture
In vitro culture
Science & Technology
topic Cellulose fiber
Hierarchical structure
Cancer cell
culture
In vitro culture
Science & Technology
description Supplementary data to this article can be found online at https://doi.org/10.1016/j.polymertesting.2019.106107.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/65839
url http://hdl.handle.net/1822/65839
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Luo, Honglin; Cui, Teng; Gan, Deqiang; Gama, F. M.; Zhang, Quanchao; Wan, Yizao, Fabrication of a novel hierarchical fibrous scaffold for breast cancer cell culture. Polymer Testing, 80(106107), 2019
0142-9418
10.1016/j.polymertesting.2019.106107
http://www.journals.elsevier.com/polymer-testing/
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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