Assessing the in vitro digestion of lactoferrin-curcumin nanoparticles using the realistic gastric model

Detalhes bibliográficos
Autor(a) principal: Madalena, Daniel Alexandre Silva
Data de Publicação: 2023
Outros Autores: Araújo, João Miguel Fernandes, Ramos, Óscar Leandro Silva, Vicente, A. A., Pinheiro, A. C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/1822/86076
Resumo: The data presented in this study are available on request from the corresponding author. The data are not publicly available due to privacy constrains.
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spelling Assessing the in vitro digestion of lactoferrin-curcumin nanoparticles using the realistic gastric modelin vitro digestiondynamic gastric digestionnanodelivery systemnanostructuresbioaccessibilityThe data presented in this study are available on request from the corresponding author. The data are not publicly available due to privacy constrains.Nanosized delivery systems have been the subject of research and discussion in the scientific community due to their unique properties and functionality. However, studies reporting the behaviour of nanodelivery systems under dynamic in vitro digestion conditions are still very scarce. To address this gap, this study aims to assess the dynamic in vitro gastric digestion of lactoferrin/curcumin nanoparticles in the realistic gastric model (RGM). For this purpose, the INFOGEST standard semi-dynamic digestion protocol was used. The nanosystems were characterized in terms of hydrodynamic size, size distribution, polydispersity index (PdI), and zeta potential using dynamic light scattering (DLS), before and during the digestion process. Confocal laser scanning microscopy (CLSM) was also used to examine particle aggregation. In addition, the release of curcumin was evaluated spectroscopically and the intrinsic fluorescence of lactoferrin was measured throughout the digestion process. The protein hydrolysis was also determined by UV-VIS-SWNIR spectroscopy to estimate, in real-time, the presence of free NH2 groups during gastric digestion. It was possible to observe that lactoferrin/curcumin nanoparticles were destabilized during the dynamic digestion process. It was also possible to conclude that low sample volumes can pose a major challenge in the application of dynamic in vitro digestion models.Daniel A. Madalena and João F. Araújo acknowledge the Foundation for Science and Technology (FCT) for their fellowships (SFRH/BD/129127/2017 and SFRH/BD/09849/2020, respectively). This work was supported by (FCT) under the scope of the strategic funding of UIDB/04469/2020 and UIDB/50016/2020 units and the I&D&I project AgriFood XXI with the operation number NORTE-01-0145-FEDER-000041, co-funded by the Fundo Europeu de Desenvolvimento Regional (FEDER) through NORTE 2020 (Programa Operacional Regional do Norte 2014/2020).info:eu-repo/semantics/publishedVersionMDPIUniversidade do MinhoMadalena, Daniel Alexandre SilvaAraújo, João Miguel FernandesRamos, Óscar Leandro SilvaVicente, A. A.Pinheiro, A. C.2023-08-022023-08-02T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/86076engMadalena, D.A.; Araújo, J.F.; Ramos, Ó.L.; Vicente, A.A.; Pinheiro, A.C. Assessing the In Vitro Digestion of Lactoferrin-Curcumin Nanoparticles Using the Realistic Gastric Model. Nanomaterials 2023, 13, 2237. https://doi.org/10.3390/nano131522372079-499110.3390/nano13152237https://www.mdpi.com/2079-4991/13/15/2237info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-08-12T01:18:31Zoai:repositorium.sdum.uminho.pt:1822/86076Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:26:42.391381Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Assessing the in vitro digestion of lactoferrin-curcumin nanoparticles using the realistic gastric model
title Assessing the in vitro digestion of lactoferrin-curcumin nanoparticles using the realistic gastric model
spellingShingle Assessing the in vitro digestion of lactoferrin-curcumin nanoparticles using the realistic gastric model
Madalena, Daniel Alexandre Silva
in vitro digestion
dynamic gastric digestion
nanodelivery system
nanostructures
bioaccessibility
title_short Assessing the in vitro digestion of lactoferrin-curcumin nanoparticles using the realistic gastric model
title_full Assessing the in vitro digestion of lactoferrin-curcumin nanoparticles using the realistic gastric model
title_fullStr Assessing the in vitro digestion of lactoferrin-curcumin nanoparticles using the realistic gastric model
title_full_unstemmed Assessing the in vitro digestion of lactoferrin-curcumin nanoparticles using the realistic gastric model
title_sort Assessing the in vitro digestion of lactoferrin-curcumin nanoparticles using the realistic gastric model
author Madalena, Daniel Alexandre Silva
author_facet Madalena, Daniel Alexandre Silva
Araújo, João Miguel Fernandes
Ramos, Óscar Leandro Silva
Vicente, A. A.
Pinheiro, A. C.
author_role author
author2 Araújo, João Miguel Fernandes
Ramos, Óscar Leandro Silva
Vicente, A. A.
Pinheiro, A. C.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Madalena, Daniel Alexandre Silva
Araújo, João Miguel Fernandes
Ramos, Óscar Leandro Silva
Vicente, A. A.
Pinheiro, A. C.
dc.subject.por.fl_str_mv in vitro digestion
dynamic gastric digestion
nanodelivery system
nanostructures
bioaccessibility
topic in vitro digestion
dynamic gastric digestion
nanodelivery system
nanostructures
bioaccessibility
description The data presented in this study are available on request from the corresponding author. The data are not publicly available due to privacy constrains.
publishDate 2023
dc.date.none.fl_str_mv 2023-08-02
2023-08-02T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/86076
url https://hdl.handle.net/1822/86076
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Madalena, D.A.; Araújo, J.F.; Ramos, Ó.L.; Vicente, A.A.; Pinheiro, A.C. Assessing the In Vitro Digestion of Lactoferrin-Curcumin Nanoparticles Using the Realistic Gastric Model. Nanomaterials 2023, 13, 2237. https://doi.org/10.3390/nano13152237
2079-4991
10.3390/nano13152237
https://www.mdpi.com/2079-4991/13/15/2237
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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