Molecular richness and biotechnological potential of bacteria cultured from Irciniidae sponges in the north-east Atlantic
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.1/11915 |
Resumo: | Several bioactive compounds originally isolated from marine sponges have been later ascribed or suggested to be synthesized by their symbionts. The cultivation of sponge-associated bacteria provides one possible route to the discovery of these metabolites. Here, we determine the bacterial richness cultured from two irciniid sponge species, Sarcotragus spinosulus and Ircinia variabilis, and ascertain their biotechnological potential. A total of 279 isolates were identified from 13 sponge specimens. These were classified into 17 genera - with Pseudovibrio, Ruegeria and Vibrio as the most dominant - and 3 to 10 putatively new bacterial species. While 16S rRNA gene sequencing identified 29 bacterial phylotypes at the 'species' level (97% sequence similarity), whole-genome BOX-PCR fingerprinting uncovered 155 genotypes, unveiling patterns of specimen-dependent occurrence of prevailing bacterial genomes across sponge individuals. Among the BOX-PCR genotypes recovered, 34% were active against clinically relevant strains, with Vibrio isolates producing the most active antagonistic effect. Several Pseudovibrio genotypes showed the presence of polyketide synthase (PKS) genes, and these were for the first time detected in isolates of the genus Aquimarina (Bacteroidetes). Our results highlight great biotechnological potential and interest for the Irciniidae sponge family and their diversified bacterial genomes. |
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Molecular richness and biotechnological potential of bacteria cultured from Irciniidae sponges in the north-east AtlanticChina sea spongesMarine spongesAntimicrobial activitiesPolyketide biosynthesisPhylogenetic diversityMediterranean spongesVertical transmissionMicrobial communityElectron microscopeAplysina aerophobaSeveral bioactive compounds originally isolated from marine sponges have been later ascribed or suggested to be synthesized by their symbionts. The cultivation of sponge-associated bacteria provides one possible route to the discovery of these metabolites. Here, we determine the bacterial richness cultured from two irciniid sponge species, Sarcotragus spinosulus and Ircinia variabilis, and ascertain their biotechnological potential. A total of 279 isolates were identified from 13 sponge specimens. These were classified into 17 genera - with Pseudovibrio, Ruegeria and Vibrio as the most dominant - and 3 to 10 putatively new bacterial species. While 16S rRNA gene sequencing identified 29 bacterial phylotypes at the 'species' level (97% sequence similarity), whole-genome BOX-PCR fingerprinting uncovered 155 genotypes, unveiling patterns of specimen-dependent occurrence of prevailing bacterial genomes across sponge individuals. Among the BOX-PCR genotypes recovered, 34% were active against clinically relevant strains, with Vibrio isolates producing the most active antagonistic effect. Several Pseudovibrio genotypes showed the presence of polyketide synthase (PKS) genes, and these were for the first time detected in isolates of the genus Aquimarina (Bacteroidetes). Our results highlight great biotechnological potential and interest for the Irciniidae sponge family and their diversified bacterial genomes.Portuguese Foundation for Science and Technology (FCT) [PTDC/MAR/101431/2008]; FCT [SFRH/BD/60873/2009, SFRH/BPD/62946/2009]Oxford Univ PressSapientiaEsteves, AnaHardoim, CristianeXavier, Joana R.Gonçalves, Jorge Manuel SantosCosta, Rodrigo2018-12-07T14:58:13Z2013-092013-09-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/11915eng0168-649610.1111/1574-6941.12140info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:23:49Zoai:sapientia.ualg.pt:10400.1/11915Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:03:21.181519Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Molecular richness and biotechnological potential of bacteria cultured from Irciniidae sponges in the north-east Atlantic |
title |
Molecular richness and biotechnological potential of bacteria cultured from Irciniidae sponges in the north-east Atlantic |
spellingShingle |
Molecular richness and biotechnological potential of bacteria cultured from Irciniidae sponges in the north-east Atlantic Esteves, Ana China sea sponges Marine sponges Antimicrobial activities Polyketide biosynthesis Phylogenetic diversity Mediterranean sponges Vertical transmission Microbial community Electron microscope Aplysina aerophoba |
title_short |
Molecular richness and biotechnological potential of bacteria cultured from Irciniidae sponges in the north-east Atlantic |
title_full |
Molecular richness and biotechnological potential of bacteria cultured from Irciniidae sponges in the north-east Atlantic |
title_fullStr |
Molecular richness and biotechnological potential of bacteria cultured from Irciniidae sponges in the north-east Atlantic |
title_full_unstemmed |
Molecular richness and biotechnological potential of bacteria cultured from Irciniidae sponges in the north-east Atlantic |
title_sort |
Molecular richness and biotechnological potential of bacteria cultured from Irciniidae sponges in the north-east Atlantic |
author |
Esteves, Ana |
author_facet |
Esteves, Ana Hardoim, Cristiane Xavier, Joana R. Gonçalves, Jorge Manuel Santos Costa, Rodrigo |
author_role |
author |
author2 |
Hardoim, Cristiane Xavier, Joana R. Gonçalves, Jorge Manuel Santos Costa, Rodrigo |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Sapientia |
dc.contributor.author.fl_str_mv |
Esteves, Ana Hardoim, Cristiane Xavier, Joana R. Gonçalves, Jorge Manuel Santos Costa, Rodrigo |
dc.subject.por.fl_str_mv |
China sea sponges Marine sponges Antimicrobial activities Polyketide biosynthesis Phylogenetic diversity Mediterranean sponges Vertical transmission Microbial community Electron microscope Aplysina aerophoba |
topic |
China sea sponges Marine sponges Antimicrobial activities Polyketide biosynthesis Phylogenetic diversity Mediterranean sponges Vertical transmission Microbial community Electron microscope Aplysina aerophoba |
description |
Several bioactive compounds originally isolated from marine sponges have been later ascribed or suggested to be synthesized by their symbionts. The cultivation of sponge-associated bacteria provides one possible route to the discovery of these metabolites. Here, we determine the bacterial richness cultured from two irciniid sponge species, Sarcotragus spinosulus and Ircinia variabilis, and ascertain their biotechnological potential. A total of 279 isolates were identified from 13 sponge specimens. These were classified into 17 genera - with Pseudovibrio, Ruegeria and Vibrio as the most dominant - and 3 to 10 putatively new bacterial species. While 16S rRNA gene sequencing identified 29 bacterial phylotypes at the 'species' level (97% sequence similarity), whole-genome BOX-PCR fingerprinting uncovered 155 genotypes, unveiling patterns of specimen-dependent occurrence of prevailing bacterial genomes across sponge individuals. Among the BOX-PCR genotypes recovered, 34% were active against clinically relevant strains, with Vibrio isolates producing the most active antagonistic effect. Several Pseudovibrio genotypes showed the presence of polyketide synthase (PKS) genes, and these were for the first time detected in isolates of the genus Aquimarina (Bacteroidetes). Our results highlight great biotechnological potential and interest for the Irciniidae sponge family and their diversified bacterial genomes. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-09 2013-09-01T00:00:00Z 2018-12-07T14:58:13Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.1/11915 |
url |
http://hdl.handle.net/10400.1/11915 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0168-6496 10.1111/1574-6941.12140 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Oxford Univ Press |
publisher.none.fl_str_mv |
Oxford Univ Press |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799133267947945984 |