MicroRNA-124-loaded nanoparticles increase survival and neuronal differentiation of neural stem cells in vitro but do not contribute to stroke outcome in vivo

Detalhes bibliográficos
Autor(a) principal: Saraiva, Cláudia
Data de Publicação: 2018
Outros Autores: Talhada, Daniela, Rai, Akhilesh, Ferreira, Raquel, Ferreira, Lino, Bernardino, Liliana, Ruscher, Karsten
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/147021
Resumo: This work was supported by FEDER funds through the POCI - COMPETE 2020 Operational Programme Competitiveness and Internationalisation in Axis I - Strengthening research, technological development and innovation (Project POCI-01-0145-FEDER-007491; Project 3386 - "StrokeTherapy") and National Funds by FCT - Foundation for Science and Technology (grant SFRH/BD/90365/2012 and Project UID/Multi/00709/2013) to LB, the Swedish Brain Fund, the Crafoord Foundation to KR, the Swedish Research Council, Sveriges Stroke Riksfbrbundet to KR, the Hans-Christian and Alice Wachtmeister Foundation and the Stiftelsen Sven-Olof Jansons livsverk to KR. This work was supported by FEDER funds through the POCI-COMPETE 2020-Operational Programme Competitiveness and Internationalisation in Axis I-Strengthening research, technological development and innovation (Project POCI-01-0145-FEDER-007491; Project 3386 - "StrokeTherapy") and National Funds by FCT-Foundation for Science and Technology (grant SFRH/BD/90365/2012 and Project UID/Multi/00709/2013), the Swedish Brain Fund, the Crafoord Foundation, the Swedish Research Council, Sveriges Stroke Riksforbundet, the Hans-Christian and Alice Wachtmeister Foundation and the Stiftelsen Sven-Olof Jansons livsverk.
id RCAP_6b0ce82b91034192d4f164353ffc1f42
oai_identifier_str oai:run.unl.pt:10362/147021
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling MicroRNA-124-loaded nanoparticles increase survival and neuronal differentiation of neural stem cells in vitro but do not contribute to stroke outcome in vivoBiochemistry, Genetics and Molecular Biology(all)Agricultural and Biological Sciences(all)SDG 3 - Good Health and Well-beingThis work was supported by FEDER funds through the POCI - COMPETE 2020 Operational Programme Competitiveness and Internationalisation in Axis I - Strengthening research, technological development and innovation (Project POCI-01-0145-FEDER-007491; Project 3386 - "StrokeTherapy") and National Funds by FCT - Foundation for Science and Technology (grant SFRH/BD/90365/2012 and Project UID/Multi/00709/2013) to LB, the Swedish Brain Fund, the Crafoord Foundation to KR, the Swedish Research Council, Sveriges Stroke Riksfbrbundet to KR, the Hans-Christian and Alice Wachtmeister Foundation and the Stiftelsen Sven-Olof Jansons livsverk to KR. This work was supported by FEDER funds through the POCI-COMPETE 2020-Operational Programme Competitiveness and Internationalisation in Axis I-Strengthening research, technological development and innovation (Project POCI-01-0145-FEDER-007491; Project 3386 - "StrokeTherapy") and National Funds by FCT-Foundation for Science and Technology (grant SFRH/BD/90365/2012 and Project UID/Multi/00709/2013), the Swedish Brain Fund, the Crafoord Foundation, the Swedish Research Council, Sveriges Stroke Riksforbundet, the Hans-Christian and Alice Wachtmeister Foundation and the Stiftelsen Sven-Olof Jansons livsverk.There is a high quest for novel therapeutic strategies to enhance recovery after stroke. MicroRNA-124 (miR-124) has been described as neuroprotective and anti-inflammatory molecule. Moreover, miR-124 is a well described enhancer of adult neurogenesis that could offer potentially beneficial effects. Herein, we used miR-124-loaded nanoparticles (miR-124 NPs) to evaluate their therapeutic potential in an in vitro and in vivo model of stroke. For that, neuroprotective and neurogenic responses were assessed in an in vitro model of stroke. Here, we found that miR-124 NPs decreased cell death and improved neuronal differentiation of subventricular zone (SVZ) neural stem cell cultures after oxygen and glucose deprivation. In contrast, intravenous injection of miR-124 NPs immediately after permanent focal ischemia induced by photothrombosis (PT) did not provide a better neurological outcome. In addition, treatment did not affect the number of 5-bromo-2’-deoxyuridine (BrdU)- and doublecortin/BrdU- positive cells in the SVZ at the study endpoint of 14 days after PT. Likewise, the ischemic insult did not affect the numbers of neuronal progenitors in the SVZ. However, in PT mice miR-124 NPs were able to specifically augment interleukin-6 levels at day 2 post-stroke. Furthermore, we also showed that NPs reached the brain parenchyma and were internalized by brain resident cells. Although, promising in vitro data could not be verified in vivo as miR-124 NPs treatment did not improve functional outcome nor presented beneficial actions on neurogenesis or post-stroke inflammation, we showed that our NP formulation can be a safe alternative for drug delivery into the brain.DQ - Departamento de QuímicaRUNSaraiva, CláudiaTalhada, DanielaRai, AkhileshFerreira, RaquelFerreira, LinoBernardino, LilianaRuscher, Karsten2023-01-05T22:11:14Z2018-03-012018-03-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/147021eng1932-6203PURE: 13554498https://doi.org/10.1371/journal.pone.0193609info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:27:59Zoai:run.unl.pt:10362/147021Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:52:44.989832Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv MicroRNA-124-loaded nanoparticles increase survival and neuronal differentiation of neural stem cells in vitro but do not contribute to stroke outcome in vivo
title MicroRNA-124-loaded nanoparticles increase survival and neuronal differentiation of neural stem cells in vitro but do not contribute to stroke outcome in vivo
spellingShingle MicroRNA-124-loaded nanoparticles increase survival and neuronal differentiation of neural stem cells in vitro but do not contribute to stroke outcome in vivo
Saraiva, Cláudia
Biochemistry, Genetics and Molecular Biology(all)
Agricultural and Biological Sciences(all)
SDG 3 - Good Health and Well-being
title_short MicroRNA-124-loaded nanoparticles increase survival and neuronal differentiation of neural stem cells in vitro but do not contribute to stroke outcome in vivo
title_full MicroRNA-124-loaded nanoparticles increase survival and neuronal differentiation of neural stem cells in vitro but do not contribute to stroke outcome in vivo
title_fullStr MicroRNA-124-loaded nanoparticles increase survival and neuronal differentiation of neural stem cells in vitro but do not contribute to stroke outcome in vivo
title_full_unstemmed MicroRNA-124-loaded nanoparticles increase survival and neuronal differentiation of neural stem cells in vitro but do not contribute to stroke outcome in vivo
title_sort MicroRNA-124-loaded nanoparticles increase survival and neuronal differentiation of neural stem cells in vitro but do not contribute to stroke outcome in vivo
author Saraiva, Cláudia
author_facet Saraiva, Cláudia
Talhada, Daniela
Rai, Akhilesh
Ferreira, Raquel
Ferreira, Lino
Bernardino, Liliana
Ruscher, Karsten
author_role author
author2 Talhada, Daniela
Rai, Akhilesh
Ferreira, Raquel
Ferreira, Lino
Bernardino, Liliana
Ruscher, Karsten
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv DQ - Departamento de Química
RUN
dc.contributor.author.fl_str_mv Saraiva, Cláudia
Talhada, Daniela
Rai, Akhilesh
Ferreira, Raquel
Ferreira, Lino
Bernardino, Liliana
Ruscher, Karsten
dc.subject.por.fl_str_mv Biochemistry, Genetics and Molecular Biology(all)
Agricultural and Biological Sciences(all)
SDG 3 - Good Health and Well-being
topic Biochemistry, Genetics and Molecular Biology(all)
Agricultural and Biological Sciences(all)
SDG 3 - Good Health and Well-being
description This work was supported by FEDER funds through the POCI - COMPETE 2020 Operational Programme Competitiveness and Internationalisation in Axis I - Strengthening research, technological development and innovation (Project POCI-01-0145-FEDER-007491; Project 3386 - "StrokeTherapy") and National Funds by FCT - Foundation for Science and Technology (grant SFRH/BD/90365/2012 and Project UID/Multi/00709/2013) to LB, the Swedish Brain Fund, the Crafoord Foundation to KR, the Swedish Research Council, Sveriges Stroke Riksfbrbundet to KR, the Hans-Christian and Alice Wachtmeister Foundation and the Stiftelsen Sven-Olof Jansons livsverk to KR. This work was supported by FEDER funds through the POCI-COMPETE 2020-Operational Programme Competitiveness and Internationalisation in Axis I-Strengthening research, technological development and innovation (Project POCI-01-0145-FEDER-007491; Project 3386 - "StrokeTherapy") and National Funds by FCT-Foundation for Science and Technology (grant SFRH/BD/90365/2012 and Project UID/Multi/00709/2013), the Swedish Brain Fund, the Crafoord Foundation, the Swedish Research Council, Sveriges Stroke Riksforbundet, the Hans-Christian and Alice Wachtmeister Foundation and the Stiftelsen Sven-Olof Jansons livsverk.
publishDate 2018
dc.date.none.fl_str_mv 2018-03-01
2018-03-01T00:00:00Z
2023-01-05T22:11:14Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/147021
url http://hdl.handle.net/10362/147021
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1932-6203
PURE: 13554498
https://doi.org/10.1371/journal.pone.0193609
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799138118406766592

Registros relacionados