Tailor-made fluorinated ionic liquids for protein delivery
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/116222 |
Resumo: | PTDC/QEQEPR/5841/2014 PTDC/QEQ-FTT/3289/2014 IF/00210/2014/CP1244/CT0003 UIDB/50006/2020 |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Tailor-made fluorinated ionic liquids for protein deliveryAggregation behaviorBiological activityDelivery systemsEncapsulation efficiencyFluorinated ionic liquidsReleaseChemical Engineering(all)Materials Science(all)PTDC/QEQEPR/5841/2014 PTDC/QEQ-FTT/3289/2014 IF/00210/2014/CP1244/CT0003 UIDB/50006/2020Nowadays, pharmaceutical companies are facing several challenges with the development and approval of new biological products. The unique properties of several fluorinated ionic liquids (FILs), such as their high surfactant power in aqueous solutions, their chemical and biological stability, and low toxicity, favor their application in the pharmaceutical industry. Furthermore, the numerous combinations between cations and anions, in the FILs design, enlarge the possibilities to construct a successful delivery system. Several FILs also proved to not affect the activity, stability, and secondary structure of the therapeutic protein lysozyme. This work aims to study the aggregation behavior of distinct FILs in the protein suitable medium, in the presence or absence of lysozyme. Besides, different incubation conditions were tested to guarantee the optimal enzymatic activity of the protein at more stable delivery systems. Following the optimization of the incubation conditions, the quantification of the encapsulated lysozyme was performed to evaluate the encapsulation efficiency of each FIL-based system. The release of the protein was tested applying variables such as time, temperature, and ultrasound frequency. The experimental results suggest that the aggregation behavior of FILs is not significantly influenced by the protein and/or protein buffer and supports their application for the design of delivery systems with high encapsulation efficiencies, maintaining the biological activity of either encapsulated and released protein.LAQV@REQUIMTEDQ - Departamento de QuímicaRUNVieira, Nicole Sofia MorgadoCastro, Paulo J.Marques, D. F.Araújo, João M. M.Pereiro, Ana Belén2021-04-26T22:58:42Z2020-08-142020-08-14T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article16application/pdfhttp://hdl.handle.net/10362/116222eng2079-4991PURE: 19798209https://doi.org/10.3390/nano10081594info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:58:41Zoai:run.unl.pt:10362/116222Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:42:59.849840Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Tailor-made fluorinated ionic liquids for protein delivery |
title |
Tailor-made fluorinated ionic liquids for protein delivery |
spellingShingle |
Tailor-made fluorinated ionic liquids for protein delivery Vieira, Nicole Sofia Morgado Aggregation behavior Biological activity Delivery systems Encapsulation efficiency Fluorinated ionic liquids Release Chemical Engineering(all) Materials Science(all) |
title_short |
Tailor-made fluorinated ionic liquids for protein delivery |
title_full |
Tailor-made fluorinated ionic liquids for protein delivery |
title_fullStr |
Tailor-made fluorinated ionic liquids for protein delivery |
title_full_unstemmed |
Tailor-made fluorinated ionic liquids for protein delivery |
title_sort |
Tailor-made fluorinated ionic liquids for protein delivery |
author |
Vieira, Nicole Sofia Morgado |
author_facet |
Vieira, Nicole Sofia Morgado Castro, Paulo J. Marques, D. F. Araújo, João M. M. Pereiro, Ana Belén |
author_role |
author |
author2 |
Castro, Paulo J. Marques, D. F. Araújo, João M. M. Pereiro, Ana Belén |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
LAQV@REQUIMTE DQ - Departamento de Química RUN |
dc.contributor.author.fl_str_mv |
Vieira, Nicole Sofia Morgado Castro, Paulo J. Marques, D. F. Araújo, João M. M. Pereiro, Ana Belén |
dc.subject.por.fl_str_mv |
Aggregation behavior Biological activity Delivery systems Encapsulation efficiency Fluorinated ionic liquids Release Chemical Engineering(all) Materials Science(all) |
topic |
Aggregation behavior Biological activity Delivery systems Encapsulation efficiency Fluorinated ionic liquids Release Chemical Engineering(all) Materials Science(all) |
description |
PTDC/QEQEPR/5841/2014 PTDC/QEQ-FTT/3289/2014 IF/00210/2014/CP1244/CT0003 UIDB/50006/2020 |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-08-14 2020-08-14T00:00:00Z 2021-04-26T22:58:42Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/116222 |
url |
http://hdl.handle.net/10362/116222 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2079-4991 PURE: 19798209 https://doi.org/10.3390/nano10081594 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
16 application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799138040536367104 |