High diversity of chickpea Mesorhizobium species isolated in a Portuguese agricultural region

Detalhes bibliográficos
Autor(a) principal: Laranjo, Marta
Data de Publicação: 2004
Outros Autores: Machado, Jorge, Young, J.Peter W., Oliveira, Solange
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10174/2105
Resumo: Chickpea rhizobia isolated from Portuguese soils were assigned to the genus Mesorhizobium by 16S-rDNA sequencing. High species diversity was found within populations of an agricultural region in the south of Portugal.;Besides the expected Mesorhizobium ciceri and M. mediterraneum, some isolates were close to M. loti or M. tianshanense and some formed a clade that may represent a new species. A new PCR-based approach, named direct amplified polymorphic DNA (DAPD) analysis, supported the 16S-based phylogeny. This suggests that this method could be used as a molecular tool to assess genetic relationships. Evaluation of genetic diversity by 16S-rDNA sequence, DAPD and protein profiles showed different levels of heterogeneity in natural populations
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spelling High diversity of chickpea Mesorhizobium species isolated in a Portuguese agricultural regionmesorhizobiumdiversityChickpea rhizobia isolated from Portuguese soils were assigned to the genus Mesorhizobium by 16S-rDNA sequencing. High species diversity was found within populations of an agricultural region in the south of Portugal.;Besides the expected Mesorhizobium ciceri and M. mediterraneum, some isolates were close to M. loti or M. tianshanense and some formed a clade that may represent a new species. A new PCR-based approach, named direct amplified polymorphic DNA (DAPD) analysis, supported the 16S-based phylogeny. This suggests that this method could be used as a molecular tool to assess genetic relationships. Evaluation of genetic diversity by 16S-rDNA sequence, DAPD and protein profiles showed different levels of heterogeneity in natural populations2010-09-27T14:47:22Z2010-09-272004-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article123648 bytesapplication/pdfhttp://hdl.handle.net/10174/2105http://hdl.handle.net/10174/2105engpag 101-10748FEMS MICROBIOLOGY ECOLOGYlivremlaranjo@uevora.ptndndismo@uevora.pt221Laranjo, MartaMachado, JorgeYoung, J.Peter W.Oliveira, Solangeinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T18:38:18Zoai:dspace.uevora.pt:10174/2105Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:57:53.281684Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv High diversity of chickpea Mesorhizobium species isolated in a Portuguese agricultural region
title High diversity of chickpea Mesorhizobium species isolated in a Portuguese agricultural region
spellingShingle High diversity of chickpea Mesorhizobium species isolated in a Portuguese agricultural region
Laranjo, Marta
mesorhizobium
diversity
title_short High diversity of chickpea Mesorhizobium species isolated in a Portuguese agricultural region
title_full High diversity of chickpea Mesorhizobium species isolated in a Portuguese agricultural region
title_fullStr High diversity of chickpea Mesorhizobium species isolated in a Portuguese agricultural region
title_full_unstemmed High diversity of chickpea Mesorhizobium species isolated in a Portuguese agricultural region
title_sort High diversity of chickpea Mesorhizobium species isolated in a Portuguese agricultural region
author Laranjo, Marta
author_facet Laranjo, Marta
Machado, Jorge
Young, J.Peter W.
Oliveira, Solange
author_role author
author2 Machado, Jorge
Young, J.Peter W.
Oliveira, Solange
author2_role author
author
author
dc.contributor.author.fl_str_mv Laranjo, Marta
Machado, Jorge
Young, J.Peter W.
Oliveira, Solange
dc.subject.por.fl_str_mv mesorhizobium
diversity
topic mesorhizobium
diversity
description Chickpea rhizobia isolated from Portuguese soils were assigned to the genus Mesorhizobium by 16S-rDNA sequencing. High species diversity was found within populations of an agricultural region in the south of Portugal.;Besides the expected Mesorhizobium ciceri and M. mediterraneum, some isolates were close to M. loti or M. tianshanense and some formed a clade that may represent a new species. A new PCR-based approach, named direct amplified polymorphic DNA (DAPD) analysis, supported the 16S-based phylogeny. This suggests that this method could be used as a molecular tool to assess genetic relationships. Evaluation of genetic diversity by 16S-rDNA sequence, DAPD and protein profiles showed different levels of heterogeneity in natural populations
publishDate 2004
dc.date.none.fl_str_mv 2004-01-01T00:00:00Z
2010-09-27T14:47:22Z
2010-09-27
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10174/2105
http://hdl.handle.net/10174/2105
url http://hdl.handle.net/10174/2105
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv pag 101-107
48
FEMS MICROBIOLOGY ECOLOGY
livre
mlaranjo@uevora.pt
nd
nd
ismo@uevora.pt
221
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eu_rights_str_mv openAccess
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instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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