Simultaneous determination of tocopherols and tocotrienols in hazelnuts by a normal phase liquid chromatographic method.

Detalhes bibliográficos
Autor(a) principal: Amaral, Joana S.
Data de Publicação: 2005
Outros Autores: Casal, Susana, Torres, Duarte, Seabra, Rosa M., Oliveira, Beatriz
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10198/627
Resumo: A normal-phase high-performance liquid chromatography (NP-HPLC) method for the determination of tocopherols and tocotrienols in hazelnuts is reported. Three extraction procedures (with and without saponification) were assayed, being the best results obtained with a simple solid-liquid extraction procedure. Chromatographic separation was achieved using an Inertsil 5 SI column using isocratic elution with n-hexane/1,4-dioxane (95.5:4.5, v/v) at a flow rate of 0.7 mL/min. The effluent was monitored by a series arrangement of a diode-array followed by a fluorescence detector. All compounds were separated in a short period of time (17 min). The method proved to be rapid, sensitive, reproducible and accurate, allowing the simultaneous determination of all vitamin E isomers.
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spelling Simultaneous determination of tocopherols and tocotrienols in hazelnuts by a normal phase liquid chromatographic method.A normal-phase high-performance liquid chromatography (NP-HPLC) method for the determination of tocopherols and tocotrienols in hazelnuts is reported. Three extraction procedures (with and without saponification) were assayed, being the best results obtained with a simple solid-liquid extraction procedure. Chromatographic separation was achieved using an Inertsil 5 SI column using isocratic elution with n-hexane/1,4-dioxane (95.5:4.5, v/v) at a flow rate of 0.7 mL/min. The effluent was monitored by a series arrangement of a diode-array followed by a fluorescence detector. All compounds were separated in a short period of time (17 min). The method proved to be rapid, sensitive, reproducible and accurate, allowing the simultaneous determination of all vitamin E isomers.The Japan Society for Analytical ChemistryBiblioteca Digital do IPBAmaral, Joana S.Casal, SusanaTorres, DuarteSeabra, Rosa M.Oliveira, Beatriz2008-05-28T09:43:25Z20052005-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/627engengAmaral, J.S.; Casal, S.; Torres, D.; Seabra, R. M.; Oliveira, M.B.P.P. (2005). Simultaneous determination of tocopherols and tocotrienols in hazelnuts by a normal phase liquid chromatographic method. Analytical Sciences. ISSN 0910-6340. 21:12, p. 1545-15480910-6340Analytical Sciences10.2116/analsci.21.1545info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:03:40Zoai:bibliotecadigital.ipb.pt:10198/627Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:54:22.189748Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Simultaneous determination of tocopherols and tocotrienols in hazelnuts by a normal phase liquid chromatographic method.
title Simultaneous determination of tocopherols and tocotrienols in hazelnuts by a normal phase liquid chromatographic method.
spellingShingle Simultaneous determination of tocopherols and tocotrienols in hazelnuts by a normal phase liquid chromatographic method.
Amaral, Joana S.
title_short Simultaneous determination of tocopherols and tocotrienols in hazelnuts by a normal phase liquid chromatographic method.
title_full Simultaneous determination of tocopherols and tocotrienols in hazelnuts by a normal phase liquid chromatographic method.
title_fullStr Simultaneous determination of tocopherols and tocotrienols in hazelnuts by a normal phase liquid chromatographic method.
title_full_unstemmed Simultaneous determination of tocopherols and tocotrienols in hazelnuts by a normal phase liquid chromatographic method.
title_sort Simultaneous determination of tocopherols and tocotrienols in hazelnuts by a normal phase liquid chromatographic method.
author Amaral, Joana S.
author_facet Amaral, Joana S.
Casal, Susana
Torres, Duarte
Seabra, Rosa M.
Oliveira, Beatriz
author_role author
author2 Casal, Susana
Torres, Duarte
Seabra, Rosa M.
Oliveira, Beatriz
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Biblioteca Digital do IPB
dc.contributor.author.fl_str_mv Amaral, Joana S.
Casal, Susana
Torres, Duarte
Seabra, Rosa M.
Oliveira, Beatriz
description A normal-phase high-performance liquid chromatography (NP-HPLC) method for the determination of tocopherols and tocotrienols in hazelnuts is reported. Three extraction procedures (with and without saponification) were assayed, being the best results obtained with a simple solid-liquid extraction procedure. Chromatographic separation was achieved using an Inertsil 5 SI column using isocratic elution with n-hexane/1,4-dioxane (95.5:4.5, v/v) at a flow rate of 0.7 mL/min. The effluent was monitored by a series arrangement of a diode-array followed by a fluorescence detector. All compounds were separated in a short period of time (17 min). The method proved to be rapid, sensitive, reproducible and accurate, allowing the simultaneous determination of all vitamin E isomers.
publishDate 2005
dc.date.none.fl_str_mv 2005
2005-01-01T00:00:00Z
2008-05-28T09:43:25Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10198/627
url http://hdl.handle.net/10198/627
dc.language.iso.fl_str_mv eng
eng
language eng
dc.relation.none.fl_str_mv Amaral, J.S.; Casal, S.; Torres, D.; Seabra, R. M.; Oliveira, M.B.P.P. (2005). Simultaneous determination of tocopherols and tocotrienols in hazelnuts by a normal phase liquid chromatographic method. Analytical Sciences. ISSN 0910-6340. 21:12, p. 1545-1548
0910-6340
Analytical Sciences
10.2116/analsci.21.1545
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv The Japan Society for Analytical Chemistry
publisher.none.fl_str_mv The Japan Society for Analytical Chemistry
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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