Assignment of individual heme EPR signalsof Desulfovibrio baculatus (strain 9974) tetraheme cytochrome c3 A redox equilibria study

Detalhes bibliográficos
Autor(a) principal: Moura, Isabel
Data de Publicação: 1988
Outros Autores: Teixeira, Miguel, Huynh, Boi H., LeGall, Jean, Moura, José J. G.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://doi.org/10.1111/j.1432-1033.1988.tb14290.x
Resumo: NIGMS NIH HHS (GM 32187)
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spelling Assignment of individual heme EPR signalsof Desulfovibrio baculatus (strain 9974) tetraheme cytochrome c3 A redox equilibria studyBiochemistryNIGMS NIH HHS (GM 32187)An EPR redox titration was performed on the tetraheme cytochrome c3 isolated from Desulfovibrio baculatus (strain 9974), a sulfate‐reducer. Using spectral differences at different poised redox states of the protein, it was possible to individualize the EPR g‐values of each of the four hemes and also to determine the mid‐point redox potentials of each individual heme: heme 4 (−70 mV) at gmax= 2.93, gmed= 2.26 and gmin= 1.51; heme 3 (− 280 mV) at gmax= 3.41; heme 2 (−300 mV) at gmax= 3.05, gmed= 2.24 and gmin= 1.34; and heme 1 (−355 mV) at gmx= 3.18. A previously described multi‐redox equilibria model used for the interpretation of NMR data of D. gigas cytochrome c3 [Santos, H., Moura, J. J. G., Moura, I., LeGall, J. & Xavier, A. V. (1984) Eur. J. Biochem. 141, 283‐296] is discussed in terms of the EPR results.Universidade NOVA de LisboaRUNMoura, IsabelTeixeira, MiguelHuynh, Boi H.LeGall, JeanMoura, José J. G.2019-09-12T22:49:40Z1988-01-011988-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article5application/pdfhttps://doi.org/10.1111/j.1432-1033.1988.tb14290.xeng0014-2956PURE: 14665999http://www.scopus.com/inward/record.url?scp=0024288629&partnerID=8YFLogxKhttps://doi.org/10.1111/j.1432-1033.1988.tb14290.xinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:35:58Zoai:run.unl.pt:10362/81032Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:36:00.378021Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Assignment of individual heme EPR signalsof Desulfovibrio baculatus (strain 9974) tetraheme cytochrome c3 A redox equilibria study
title Assignment of individual heme EPR signalsof Desulfovibrio baculatus (strain 9974) tetraheme cytochrome c3 A redox equilibria study
spellingShingle Assignment of individual heme EPR signalsof Desulfovibrio baculatus (strain 9974) tetraheme cytochrome c3 A redox equilibria study
Moura, Isabel
Biochemistry
title_short Assignment of individual heme EPR signalsof Desulfovibrio baculatus (strain 9974) tetraheme cytochrome c3 A redox equilibria study
title_full Assignment of individual heme EPR signalsof Desulfovibrio baculatus (strain 9974) tetraheme cytochrome c3 A redox equilibria study
title_fullStr Assignment of individual heme EPR signalsof Desulfovibrio baculatus (strain 9974) tetraheme cytochrome c3 A redox equilibria study
title_full_unstemmed Assignment of individual heme EPR signalsof Desulfovibrio baculatus (strain 9974) tetraheme cytochrome c3 A redox equilibria study
title_sort Assignment of individual heme EPR signalsof Desulfovibrio baculatus (strain 9974) tetraheme cytochrome c3 A redox equilibria study
author Moura, Isabel
author_facet Moura, Isabel
Teixeira, Miguel
Huynh, Boi H.
LeGall, Jean
Moura, José J. G.
author_role author
author2 Teixeira, Miguel
Huynh, Boi H.
LeGall, Jean
Moura, José J. G.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade NOVA de Lisboa
RUN
dc.contributor.author.fl_str_mv Moura, Isabel
Teixeira, Miguel
Huynh, Boi H.
LeGall, Jean
Moura, José J. G.
dc.subject.por.fl_str_mv Biochemistry
topic Biochemistry
description NIGMS NIH HHS (GM 32187)
publishDate 1988
dc.date.none.fl_str_mv 1988-01-01
1988-01-01T00:00:00Z
2019-09-12T22:49:40Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
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dc.identifier.uri.fl_str_mv https://doi.org/10.1111/j.1432-1033.1988.tb14290.x
url https://doi.org/10.1111/j.1432-1033.1988.tb14290.x
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0014-2956
PURE: 14665999
http://www.scopus.com/inward/record.url?scp=0024288629&partnerID=8YFLogxK
https://doi.org/10.1111/j.1432-1033.1988.tb14290.x
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instacron:RCAAP
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