Assignment of individual heme EPR signalsof Desulfovibrio baculatus (strain 9974) tetraheme cytochrome c3 A redox equilibria study
Autor(a) principal: | |
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Data de Publicação: | 1988 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://doi.org/10.1111/j.1432-1033.1988.tb14290.x |
Resumo: | NIGMS NIH HHS (GM 32187) |
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Assignment of individual heme EPR signalsof Desulfovibrio baculatus (strain 9974) tetraheme cytochrome c3 A redox equilibria studyBiochemistryNIGMS NIH HHS (GM 32187)An EPR redox titration was performed on the tetraheme cytochrome c3 isolated from Desulfovibrio baculatus (strain 9974), a sulfate‐reducer. Using spectral differences at different poised redox states of the protein, it was possible to individualize the EPR g‐values of each of the four hemes and also to determine the mid‐point redox potentials of each individual heme: heme 4 (−70 mV) at gmax= 2.93, gmed= 2.26 and gmin= 1.51; heme 3 (− 280 mV) at gmax= 3.41; heme 2 (−300 mV) at gmax= 3.05, gmed= 2.24 and gmin= 1.34; and heme 1 (−355 mV) at gmx= 3.18. A previously described multi‐redox equilibria model used for the interpretation of NMR data of D. gigas cytochrome c3 [Santos, H., Moura, J. J. G., Moura, I., LeGall, J. & Xavier, A. V. (1984) Eur. J. Biochem. 141, 283‐296] is discussed in terms of the EPR results.Universidade NOVA de LisboaRUNMoura, IsabelTeixeira, MiguelHuynh, Boi H.LeGall, JeanMoura, José J. G.2019-09-12T22:49:40Z1988-01-011988-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article5application/pdfhttps://doi.org/10.1111/j.1432-1033.1988.tb14290.xeng0014-2956PURE: 14665999http://www.scopus.com/inward/record.url?scp=0024288629&partnerID=8YFLogxKhttps://doi.org/10.1111/j.1432-1033.1988.tb14290.xinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:35:58Zoai:run.unl.pt:10362/81032Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:36:00.378021Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Assignment of individual heme EPR signalsof Desulfovibrio baculatus (strain 9974) tetraheme cytochrome c3 A redox equilibria study |
title |
Assignment of individual heme EPR signalsof Desulfovibrio baculatus (strain 9974) tetraheme cytochrome c3 A redox equilibria study |
spellingShingle |
Assignment of individual heme EPR signalsof Desulfovibrio baculatus (strain 9974) tetraheme cytochrome c3 A redox equilibria study Moura, Isabel Biochemistry |
title_short |
Assignment of individual heme EPR signalsof Desulfovibrio baculatus (strain 9974) tetraheme cytochrome c3 A redox equilibria study |
title_full |
Assignment of individual heme EPR signalsof Desulfovibrio baculatus (strain 9974) tetraheme cytochrome c3 A redox equilibria study |
title_fullStr |
Assignment of individual heme EPR signalsof Desulfovibrio baculatus (strain 9974) tetraheme cytochrome c3 A redox equilibria study |
title_full_unstemmed |
Assignment of individual heme EPR signalsof Desulfovibrio baculatus (strain 9974) tetraheme cytochrome c3 A redox equilibria study |
title_sort |
Assignment of individual heme EPR signalsof Desulfovibrio baculatus (strain 9974) tetraheme cytochrome c3 A redox equilibria study |
author |
Moura, Isabel |
author_facet |
Moura, Isabel Teixeira, Miguel Huynh, Boi H. LeGall, Jean Moura, José J. G. |
author_role |
author |
author2 |
Teixeira, Miguel Huynh, Boi H. LeGall, Jean Moura, José J. G. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade NOVA de Lisboa RUN |
dc.contributor.author.fl_str_mv |
Moura, Isabel Teixeira, Miguel Huynh, Boi H. LeGall, Jean Moura, José J. G. |
dc.subject.por.fl_str_mv |
Biochemistry |
topic |
Biochemistry |
description |
NIGMS NIH HHS (GM 32187) |
publishDate |
1988 |
dc.date.none.fl_str_mv |
1988-01-01 1988-01-01T00:00:00Z 2019-09-12T22:49:40Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.1111/j.1432-1033.1988.tb14290.x |
url |
https://doi.org/10.1111/j.1432-1033.1988.tb14290.x |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0014-2956 PURE: 14665999 http://www.scopus.com/inward/record.url?scp=0024288629&partnerID=8YFLogxK https://doi.org/10.1111/j.1432-1033.1988.tb14290.x |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
5 application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799137980020948992 |