Comparison of Leishmania typing results obtained from 16 European clinical laboratories in 2014

Detalhes bibliográficos
Autor(a) principal: Van der Auwera, Gert
Data de Publicação: 2016
Outros Autores: Bart, Aldert, Chicharro, Carmen, Cortes, Sofia, Davidsson, Leigh, Di Muccio, Trentina, Dujardin, Jean-Claude, Felger, Ingrid, Paglia, Maria Grazia, Grimm, Felix, Harms, Gundel, Jaffe, Charles L., Manser, Monika, Ravel, Christophe, Robert-Gangneux, Florence, Roelfsema, Jeroen, Töz, Seray, Verweij, Jaco J, Chiodini, Peter L
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/36621
Resumo: This article is copyright of The Authors, 2016.
id RCAP_6fda47f7eaf9273c2568fe1749055f27
oai_identifier_str oai:run.unl.pt:10362/36621
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Comparison of Leishmania typing results obtained from 16 European clinical laboratories in 2014LeishmaniasisTypinghsp70rDNA ITS1Mini-exonKinetoplast DNAParasitologyInfectious DiseasesSDG 3 - Good Health and Well-beingThis article is copyright of The Authors, 2016.Leishmaniasis is endemic in southern Europe, and in other European countries cases are diagnosed in travellers who have visited affected areas both within the continent and beyond. Prompt and accurate diagnosis poses a challenge in clinical practice in Europe. Different methods exist for identification of the infecting Leishmania species. Sixteen clinical laboratories in 10 European countries, plus Israel and Turkey, conducted a study to assess their genotyping performance. DNA from 21 promastigote cultures of 13 species was analysed blindly by the routinely used typing method. Five different molecular targets were used, which were analysed with PCR-based methods. Different levels of identification were achieved, and either the Leishmania subgenus, species complex, or actual species were reported. The overall error rate of strains placed in the wrong complex or species was 8.5%. Various reasons for incorrect typing were identified. The study shows there is considerable room for improvement and standardisation of Leishmania typing. The use of well validated standard operating procedures is recommended, covering testing, interpretation, and reporting guidelines. Application of the internal transcribed spacer 1 of the rDNA array should be restricted to Old World samples, while the heat-shock protein 70 gene and the mini-exon can be applied globally.Instituto de Higiene e Medicina Tropical (IHMT)Vector borne diseases and pathogens (VBD)Global Health and Tropical Medicine (GHTM)RUNVan der Auwera, GertBart, AldertChicharro, CarmenCortes, SofiaDavidsson, LeighDi Muccio, TrentinaDujardin, Jean-ClaudeFelger, IngridPaglia, Maria GraziaGrimm, FelixHarms, GundelJaffe, Charles L.Manser, MonikaRavel, ChristopheRobert-Gangneux, FlorenceRoelfsema, JeroenTöz, SerayVerweij, Jaco JChiodini, Peter L2018-05-11T22:05:04Z2016-12-082016-12-08T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article11application/pdfhttp://hdl.handle.net/10362/36621eng1025-496XPURE: 2381766https://doi.org/10.2807/1560-7917.ES.2016.21.49.30418info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:20:06Zoai:run.unl.pt:10362/36621Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:30:35.552598Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Comparison of Leishmania typing results obtained from 16 European clinical laboratories in 2014
title Comparison of Leishmania typing results obtained from 16 European clinical laboratories in 2014
spellingShingle Comparison of Leishmania typing results obtained from 16 European clinical laboratories in 2014
Van der Auwera, Gert
Leishmaniasis
Typing
hsp70
rDNA ITS1
Mini-exon
Kinetoplast DNA
Parasitology
Infectious Diseases
SDG 3 - Good Health and Well-being
title_short Comparison of Leishmania typing results obtained from 16 European clinical laboratories in 2014
title_full Comparison of Leishmania typing results obtained from 16 European clinical laboratories in 2014
title_fullStr Comparison of Leishmania typing results obtained from 16 European clinical laboratories in 2014
title_full_unstemmed Comparison of Leishmania typing results obtained from 16 European clinical laboratories in 2014
title_sort Comparison of Leishmania typing results obtained from 16 European clinical laboratories in 2014
author Van der Auwera, Gert
author_facet Van der Auwera, Gert
Bart, Aldert
Chicharro, Carmen
Cortes, Sofia
Davidsson, Leigh
Di Muccio, Trentina
Dujardin, Jean-Claude
Felger, Ingrid
Paglia, Maria Grazia
Grimm, Felix
Harms, Gundel
Jaffe, Charles L.
Manser, Monika
Ravel, Christophe
Robert-Gangneux, Florence
Roelfsema, Jeroen
Töz, Seray
Verweij, Jaco J
Chiodini, Peter L
author_role author
author2 Bart, Aldert
Chicharro, Carmen
Cortes, Sofia
Davidsson, Leigh
Di Muccio, Trentina
Dujardin, Jean-Claude
Felger, Ingrid
Paglia, Maria Grazia
Grimm, Felix
Harms, Gundel
Jaffe, Charles L.
Manser, Monika
Ravel, Christophe
Robert-Gangneux, Florence
Roelfsema, Jeroen
Töz, Seray
Verweij, Jaco J
Chiodini, Peter L
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Higiene e Medicina Tropical (IHMT)
Vector borne diseases and pathogens (VBD)
Global Health and Tropical Medicine (GHTM)
RUN
dc.contributor.author.fl_str_mv Van der Auwera, Gert
Bart, Aldert
Chicharro, Carmen
Cortes, Sofia
Davidsson, Leigh
Di Muccio, Trentina
Dujardin, Jean-Claude
Felger, Ingrid
Paglia, Maria Grazia
Grimm, Felix
Harms, Gundel
Jaffe, Charles L.
Manser, Monika
Ravel, Christophe
Robert-Gangneux, Florence
Roelfsema, Jeroen
Töz, Seray
Verweij, Jaco J
Chiodini, Peter L
dc.subject.por.fl_str_mv Leishmaniasis
Typing
hsp70
rDNA ITS1
Mini-exon
Kinetoplast DNA
Parasitology
Infectious Diseases
SDG 3 - Good Health and Well-being
topic Leishmaniasis
Typing
hsp70
rDNA ITS1
Mini-exon
Kinetoplast DNA
Parasitology
Infectious Diseases
SDG 3 - Good Health and Well-being
description This article is copyright of The Authors, 2016.
publishDate 2016
dc.date.none.fl_str_mv 2016-12-08
2016-12-08T00:00:00Z
2018-05-11T22:05:04Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/36621
url http://hdl.handle.net/10362/36621
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1025-496X
PURE: 2381766
https://doi.org/10.2807/1560-7917.ES.2016.21.49.30418
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 11
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799137929856024576

Registros relacionados