Comparative proteomic analysis of nodulated and non-nodulated casuarina glauca sieb. Ex spreng. grown under salinity conditions using sequential window acquisition of all theoretical mass spectra (SWATH-MS)

Detalhes bibliográficos
Autor(a) principal: Graça, Inês
Data de Publicação: 2020
Outros Autores: Mendes, Vera M., Marques, Isabel, Duro, Nuno, da Costa, Mário, Ramalho, José C., Pawlowski, Katharina, Manadas, Bruno, Pinto Ricardo, Cândido P., Ribeiro-Barros, Ana Isabel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/114689
Resumo: POCI-01-0145-FEDER-031999 UID/AGR/04129/2019 UID/GEO/04035/2019 POCI-01-0145-FEDER-402-022125 (ref.: ROTEIRO/0028/2013
id RCAP_6fe179a2ebd1a77f8e7b4b86d7616f14
oai_identifier_str oai:run.unl.pt:10362/114689
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Comparative proteomic analysis of nodulated and non-nodulated casuarina glauca sieb. Ex spreng. grown under salinity conditions using sequential window acquisition of all theoretical mass spectra (SWATH-MS)Actinorhizal plantsCasuarina glaucaFrankiaProteomicsSalt stressCatalysisMolecular BiologySpectroscopyComputer Science ApplicationsPhysical and Theoretical ChemistryOrganic ChemistryInorganic ChemistryPOCI-01-0145-FEDER-031999 UID/AGR/04129/2019 UID/GEO/04035/2019 POCI-01-0145-FEDER-402-022125 (ref.: ROTEIRO/0028/2013Casuarina glauca displays high levels of salt tolerance, but very little is known about how this tree adapts to saline conditions. To understand the molecular basis of C. glauca response to salt stress, we have analyzed the proteome from branchlets of plants nodulated by nitrogen-fixing Frankia Thr bacteria (NOD+) and non-nodulated plants supplied with KNO3 (KNO3+), exposed to 0, 200, 400, and 600 mM NaCl. Proteins were identified by Short Gel, Long Gradient Liquid Chromatography coupled to Tandem Mass Spectrometry and quantified by Sequential Window Acquisition of All Theoretical Mass Spectra -Mass Spectrometry. 600 proteins were identified and 357 quantified. Differentially Expressed Proteins (DEPs) were multifunctional and mainly involved in Carbohydrate Metabolism, Cellular Processes, and Environmental Information Processing. The number of DEPs increased gradually with stress severity: (i) from 7 (200 mM NaCl) to 40 (600 mM NaCl) in KNO3+; and (ii) from 6 (200 mM NaCl) to 23 (600 mM NaCl) in NOD+. Protein–protein interaction analysis identified different interacting proteins involved in general metabolic pathways as well as in the biosynthesis of secondary metabolites with different response networks related to salt stress. Salt tolerance in C. glauca is related to a moderate impact on the photosynthetic machinery (one of the first and most important stress targets) as well as to an enhancement of the antioxidant status that maintains cellular homeostasis.Instituto de Tecnologia Química e Biológica António Xavier (ITQB)GeoBioTec - Geobiociências, Geoengenharias e GeotecnologiasRUNGraça, InêsMendes, Vera M.Marques, IsabelDuro, Nunoda Costa, MárioRamalho, José C.Pawlowski, KatharinaManadas, BrunoPinto Ricardo, Cândido P.Ribeiro-Barros, Ana Isabel2021-03-29T22:24:35Z2020-01-012020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/114689eng1661-6596PURE: 28003003https://doi.org/10.3390/ijms21010078info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:57:22Zoai:run.unl.pt:10362/114689Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:42:36.448159Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Comparative proteomic analysis of nodulated and non-nodulated casuarina glauca sieb. Ex spreng. grown under salinity conditions using sequential window acquisition of all theoretical mass spectra (SWATH-MS)
title Comparative proteomic analysis of nodulated and non-nodulated casuarina glauca sieb. Ex spreng. grown under salinity conditions using sequential window acquisition of all theoretical mass spectra (SWATH-MS)
spellingShingle Comparative proteomic analysis of nodulated and non-nodulated casuarina glauca sieb. Ex spreng. grown under salinity conditions using sequential window acquisition of all theoretical mass spectra (SWATH-MS)
Graça, Inês
Actinorhizal plants
Casuarina glauca
Frankia
Proteomics
Salt stress
Catalysis
Molecular Biology
Spectroscopy
Computer Science Applications
Physical and Theoretical Chemistry
Organic Chemistry
Inorganic Chemistry
title_short Comparative proteomic analysis of nodulated and non-nodulated casuarina glauca sieb. Ex spreng. grown under salinity conditions using sequential window acquisition of all theoretical mass spectra (SWATH-MS)
title_full Comparative proteomic analysis of nodulated and non-nodulated casuarina glauca sieb. Ex spreng. grown under salinity conditions using sequential window acquisition of all theoretical mass spectra (SWATH-MS)
title_fullStr Comparative proteomic analysis of nodulated and non-nodulated casuarina glauca sieb. Ex spreng. grown under salinity conditions using sequential window acquisition of all theoretical mass spectra (SWATH-MS)
title_full_unstemmed Comparative proteomic analysis of nodulated and non-nodulated casuarina glauca sieb. Ex spreng. grown under salinity conditions using sequential window acquisition of all theoretical mass spectra (SWATH-MS)
title_sort Comparative proteomic analysis of nodulated and non-nodulated casuarina glauca sieb. Ex spreng. grown under salinity conditions using sequential window acquisition of all theoretical mass spectra (SWATH-MS)
author Graça, Inês
author_facet Graça, Inês
Mendes, Vera M.
Marques, Isabel
Duro, Nuno
da Costa, Mário
Ramalho, José C.
Pawlowski, Katharina
Manadas, Bruno
Pinto Ricardo, Cândido P.
Ribeiro-Barros, Ana Isabel
author_role author
author2 Mendes, Vera M.
Marques, Isabel
Duro, Nuno
da Costa, Mário
Ramalho, José C.
Pawlowski, Katharina
Manadas, Bruno
Pinto Ricardo, Cândido P.
Ribeiro-Barros, Ana Isabel
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Tecnologia Química e Biológica António Xavier (ITQB)
GeoBioTec - Geobiociências, Geoengenharias e Geotecnologias
RUN
dc.contributor.author.fl_str_mv Graça, Inês
Mendes, Vera M.
Marques, Isabel
Duro, Nuno
da Costa, Mário
Ramalho, José C.
Pawlowski, Katharina
Manadas, Bruno
Pinto Ricardo, Cândido P.
Ribeiro-Barros, Ana Isabel
dc.subject.por.fl_str_mv Actinorhizal plants
Casuarina glauca
Frankia
Proteomics
Salt stress
Catalysis
Molecular Biology
Spectroscopy
Computer Science Applications
Physical and Theoretical Chemistry
Organic Chemistry
Inorganic Chemistry
topic Actinorhizal plants
Casuarina glauca
Frankia
Proteomics
Salt stress
Catalysis
Molecular Biology
Spectroscopy
Computer Science Applications
Physical and Theoretical Chemistry
Organic Chemistry
Inorganic Chemistry
description POCI-01-0145-FEDER-031999 UID/AGR/04129/2019 UID/GEO/04035/2019 POCI-01-0145-FEDER-402-022125 (ref.: ROTEIRO/0028/2013
publishDate 2020
dc.date.none.fl_str_mv 2020-01-01
2020-01-01T00:00:00Z
2021-03-29T22:24:35Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/114689
url http://hdl.handle.net/10362/114689
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1661-6596
PURE: 28003003
https://doi.org/10.3390/ijms21010078
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799138036987985920

Registros relacionados