Identification of field pea cultivars (Pisum sativum L.) using micro-satellite molecular markers
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.11/1616 |
Resumo: | Conventionally morphological descriptors are routinely used for establishing the identity of varieties. This kind of descriptors has some disadvantages, namely most of them are quantitative, controlled by several pairs of genes, and their expression is infl uenced by environmental factors. Molecular markers have a potential to facilitate this procedure, increase the reliability of decisions, and substantially save the time and space needed for experiments. In this study we intended to identify 20 cultivars of fi eld pea (Pisum sativum L.), registered in the Community Catalog of Varieties, by microsatellites molecular markers. After DNA extraction, seven different loci were analyzed. PCR amplifi cations were conducted and the resulting fragments were separated on a 3.5% MS-8 agarose gel in TBE buffer. The gels were analyzed for the presence/absence of bands and a table with binary code was made. For each locus PIC value was calculated. The data were processed with the statistical software NTSYS-pc, using the SIMQUAL module and Jaccard similarity coeffi cient, followed by UPGMA cluster analysis. With the analysis of six polymorphic loci was possible to distinguish almost all of cultivars. The most informative loci were AD61 and AB53. The UPGMA dendrogram showed two main groups. The results showed a high potential and resolving power of SSR markers in distinct assessment. SSR markers might also be useful in germplasm management and genetic diversity studies. In the present research work, we have successfully employed high resolution agarose gel electrophoresis for genotyping with microsatellite markers in pea. |
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Identification of field pea cultivars (Pisum sativum L.) using micro-satellite molecular markersCultivar identificationDNA fingerprintingHigh resolution agarose gelConventionally morphological descriptors are routinely used for establishing the identity of varieties. This kind of descriptors has some disadvantages, namely most of them are quantitative, controlled by several pairs of genes, and their expression is infl uenced by environmental factors. Molecular markers have a potential to facilitate this procedure, increase the reliability of decisions, and substantially save the time and space needed for experiments. In this study we intended to identify 20 cultivars of fi eld pea (Pisum sativum L.), registered in the Community Catalog of Varieties, by microsatellites molecular markers. After DNA extraction, seven different loci were analyzed. PCR amplifi cations were conducted and the resulting fragments were separated on a 3.5% MS-8 agarose gel in TBE buffer. The gels were analyzed for the presence/absence of bands and a table with binary code was made. For each locus PIC value was calculated. The data were processed with the statistical software NTSYS-pc, using the SIMQUAL module and Jaccard similarity coeffi cient, followed by UPGMA cluster analysis. With the analysis of six polymorphic loci was possible to distinguish almost all of cultivars. The most informative loci were AD61 and AB53. The UPGMA dendrogram showed two main groups. The results showed a high potential and resolving power of SSR markers in distinct assessment. SSR markers might also be useful in germplasm management and genetic diversity studies. In the present research work, we have successfully employed high resolution agarose gel electrophoresis for genotyping with microsatellite markers in pea.FEDERInstitute of Plant Physiology and Genetics – Bulgarian Academy of SciencesRepositório Científico do Instituto Politécnico de Castelo BrancoReis, C.M.G.Diogo, M.G.2012-12-21T16:09:07Z20122012-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.11/1616engREIS, C.M.G. ; DIOGO, M.G. (2012) - Identification of field pea cultivars (Pisum sativum L.) using micro-satellite molecular markers. In Genetics and Plant Physiology. ISSN ISSN 1314-6394. Vol. 2, n.º 1-2. p. 57-63.ISSN 1314-6394info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-01-16T11:38:58Zoai:repositorio.ipcb.pt:10400.11/1616Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:33:04.784392Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Identification of field pea cultivars (Pisum sativum L.) using micro-satellite molecular markers |
title |
Identification of field pea cultivars (Pisum sativum L.) using micro-satellite molecular markers |
spellingShingle |
Identification of field pea cultivars (Pisum sativum L.) using micro-satellite molecular markers Reis, C.M.G. Cultivar identification DNA fingerprinting High resolution agarose gel |
title_short |
Identification of field pea cultivars (Pisum sativum L.) using micro-satellite molecular markers |
title_full |
Identification of field pea cultivars (Pisum sativum L.) using micro-satellite molecular markers |
title_fullStr |
Identification of field pea cultivars (Pisum sativum L.) using micro-satellite molecular markers |
title_full_unstemmed |
Identification of field pea cultivars (Pisum sativum L.) using micro-satellite molecular markers |
title_sort |
Identification of field pea cultivars (Pisum sativum L.) using micro-satellite molecular markers |
author |
Reis, C.M.G. |
author_facet |
Reis, C.M.G. Diogo, M.G. |
author_role |
author |
author2 |
Diogo, M.G. |
author2_role |
author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Politécnico de Castelo Branco |
dc.contributor.author.fl_str_mv |
Reis, C.M.G. Diogo, M.G. |
dc.subject.por.fl_str_mv |
Cultivar identification DNA fingerprinting High resolution agarose gel |
topic |
Cultivar identification DNA fingerprinting High resolution agarose gel |
description |
Conventionally morphological descriptors are routinely used for establishing the identity of varieties. This kind of descriptors has some disadvantages, namely most of them are quantitative, controlled by several pairs of genes, and their expression is infl uenced by environmental factors. Molecular markers have a potential to facilitate this procedure, increase the reliability of decisions, and substantially save the time and space needed for experiments. In this study we intended to identify 20 cultivars of fi eld pea (Pisum sativum L.), registered in the Community Catalog of Varieties, by microsatellites molecular markers. After DNA extraction, seven different loci were analyzed. PCR amplifi cations were conducted and the resulting fragments were separated on a 3.5% MS-8 agarose gel in TBE buffer. The gels were analyzed for the presence/absence of bands and a table with binary code was made. For each locus PIC value was calculated. The data were processed with the statistical software NTSYS-pc, using the SIMQUAL module and Jaccard similarity coeffi cient, followed by UPGMA cluster analysis. With the analysis of six polymorphic loci was possible to distinguish almost all of cultivars. The most informative loci were AD61 and AB53. The UPGMA dendrogram showed two main groups. The results showed a high potential and resolving power of SSR markers in distinct assessment. SSR markers might also be useful in germplasm management and genetic diversity studies. In the present research work, we have successfully employed high resolution agarose gel electrophoresis for genotyping with microsatellite markers in pea. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-12-21T16:09:07Z 2012 2012-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.11/1616 |
url |
http://hdl.handle.net/10400.11/1616 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
REIS, C.M.G. ; DIOGO, M.G. (2012) - Identification of field pea cultivars (Pisum sativum L.) using micro-satellite molecular markers. In Genetics and Plant Physiology. ISSN ISSN 1314-6394. Vol. 2, n.º 1-2. p. 57-63. ISSN 1314-6394 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Institute of Plant Physiology and Genetics – Bulgarian Academy of Sciences |
publisher.none.fl_str_mv |
Institute of Plant Physiology and Genetics – Bulgarian Academy of Sciences |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799130788622499840 |